A fiber optoacoustic emitter with controlled ultrasound frequency for cell membrane sonoporation at submillimeter spatial resolution.

Published on Sep 30, 2020in Photoacoustics8.484
· DOI :10.1016/J.PACS.2020.100208
Linli Shi1
Estimated H-index: 1
(BU: Boston University),
Ying Jiang4
Estimated H-index: 4
(BU: Boston University)
+ 4 AuthorsChen Yang74
Estimated H-index: 74
(BU: Boston University)
Sources
Abstract
Abstract Focused ultrasound has attracted great attention in minimally invasive therapeutic and mechanism studies. Frequency below 1 MHz is identified preferable for high-efficiency bio-modulation. However, the poor spatial confinement of several millimeters and large device diameter of ∼25 mm of typical sub-MHz ultrasound technology suffered from the diffraction limit, severely hindering its further applications. To address it, a fiber-based optoacoustic emitter (FOE) is developed, serving as a miniaturized ultrasound point source, with sub-millimeter confinement, composed of an optical diffusion layer and an expansion layer on an optical fiber. By modifying acoustic damping and light absorption performance, controllable frequencies in the range of 0.083 MHz to 5.500 MHz are achieved and further induce cell membrane sonoporation with frequency dependent efficiency. By solving the problem of compromise between sub-MHz frequency and sub-millimeter precision via breaking the diffraction limit, the FOE shows a great potential in region-specific drug delivery, gene transfection and neurostimulation.
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#1Ying Jiang (BU: Boston University)H-Index: 4
#2Hyeon Jeong LeeH-Index: 11
Last. Ji-Xin ChengH-Index: 84
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Low-intensity ultrasound is an emerging modality for neuromodulation. Yet, transcranial neuromodulation using low-frequency piezo-based transducers offers poor spatial confinement of excitation volume, often bigger than a few millimeters in diameter. In addition, the bulky size limits their implementation in a wearable setting and prevents integration with other experimental modalities. Here, we report spatially confined optoacoustic neural stimulation through a miniaturized Fiber-Optoacoustic C...
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#1Reine-Marie Guillermic (UM: University of Manitoba)H-Index: 11
#2Maxime Lanoy (UM: University of Manitoba)H-Index: 6
Last. John H. Page (UM: University of Manitoba)H-Index: 37
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Abstract Having a material that is matched in acoustic impedance with the surrounding medium is a considerable asset for many underwater acoustic applications. In this work, impedance matching is achieved by dispersing small, deeply subwavelength sized particles in a soft matrix, and the appropriate concentration is determined with the help of Coherent Potential Approximation and Waterman & Truell models. We show experimentally the validity of the models using mixtures of Polydimethylsiloxane (P...
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#1Davide Folloni (University of Oxford)H-Index: 12
#2Lennart Verhagen (University of Oxford)H-Index: 30
Last. Jerome Sallet (University of Oxford)H-Index: 35
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Summary The causal role of an area within a neural network can be determined by interfering with its activity and measuring the impact. Many current reversible manipulation techniques have limitations preventing their application, particularly in deep areas of the primate brain. Here, we demonstrate that a focused transcranial ultrasound stimulation (TUS) protocol impacts activity even in deep brain areas: a subcortical brain structure, the amygdala (experiment 1), and a deep cortical region, th...
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#1Taehwa Lee (UM: University of Michigan)H-Index: 17
#2Hyoung Won Baac (SKKU: Sungkyunkwan University)H-Index: 17
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#1Jerzy O. Szablowski (California Institute of Technology)H-Index: 12
#2Audrey Lee-Gosselin (California Institute of Technology)H-Index: 13
Last. Mikhail G. Shapiro (California Institute of Technology)H-Index: 26
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Neurological and psychiatric disorders are often characterized by dysfunctional neural circuits in specific regions of the brain. Existing treatment strategies, including the use of drugs and implantable brain stimulators, aim to modulate the activity of these circuits. However, they are not cell-type-specific, lack spatial targeting or require invasive procedures. Here, we report a cell-type-specific and non-invasive approach based on acoustically targeted chemogenetics that enables the modulat...
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#1William J. Tyler (ASU: Arizona State University)H-Index: 28
#2Shane Lani (APL: Johns Hopkins University Applied Physics Laboratory)H-Index: 7
Last. Grace M. Hwang (APL: Johns Hopkins University Applied Physics Laboratory)H-Index: 13
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Ultrasound (US) is recognized for its use in medical imaging as a diagnostic tool. As an acoustic energy source, US has become increasingly appreciated over the past decade for its ability to non-invasively modulate cellular activity including neuronal activity. Data obtained from a host of experimental models has shown that low-intensity US can reversibly modulate the physiological activity of neurons in peripheral nerves, spinal cord, and intact brain circuits. Experimental evidence indicates ...
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#1Lu Lan (BU: Boston University)H-Index: 14
#2Yan XiaH-Index: 2
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 84
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Lumpectomy, also called breast-conserving surgery, has become the standard surgical treatment for early-stage breast cancer. However, accurately locating the tumor during a lumpectomy, especially when the lesion is small and nonpalpable, is a challenge. Such difficulty can lead to either incomplete tumor removal or prolonged surgical time, which result in high re-operation rates (~25%) and increased surgical costs. Here, we report a fiber optoacoustic guide (FOG) with augmented reality (AR) for ...
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#2Richard J. Colchester (UCL: University College London)H-Index: 10
Last. Adrien E. Desjardins (UCL: University College London)H-Index: 31
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Polydimethylsiloxane (PDMS) is widely used in biomedical science and can form composites that have broad applicability. One promising application where PDMS composites offer several advantages is optical ultrasound generation via the photoacoustic effect. Here, methods to create these PDMS composites are reviewed and classified. It is highlighted how the composites can be applied to a range of substrates, from micrometer‐scale, temperature‐sensitive optical fibers to centimeter‐scale curved and ...
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Abstract Cavitation-facilitated microbubble-mediated focused ultrasound therapy is a promising method of drug delivery across the blood–brain barrier (BBB) for treating many neurological disorders. Unlike ultrasound thermal therapies, during which magnetic resonance thermometry can serve as a reliable treatment control modality, real-time control of modulated BBB disruption with undetectable vascular damage remains a challenge. Here a closed-loop cavitation controlling paradigm that sustains sta...
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#1Tam Thanh Nguyen (Nagoya University)H-Index: 4
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Last. Keiji Yasuda (Nagoya University)H-Index: 17
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Abstract Cavitation, chemical effect, and mechanical effect thresholds were investigated in wide frequency ranges from 22 to 4880 kHz. Each threshold was measured in terms of sound pressure at fundamental frequency. Broadband noise emitted from acoustic cavitation bubbles was detected by a hydrophone to determine the cavitation threshold. Potassium iodide oxidation caused by acoustic cavitation was used to quantify the chemical effect threshold. The ultrasonic erosion of aluminum foil was conduc...
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#1Haobo Zhu (HUST: Huazhong University of Science and Technology)H-Index: 2
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Abstract null null Aiming to provide dynamically adjustable acoustic field for in vivo ultrasound application scenarios, a miniaturized optical fiber photoacoustic (PA) transmitter is presented. Different from the existing laser generated focused ultrasound probes with fixed acoustic focal length, its focus can be continuously adjusted from infinity to 2.166 mm by deforming the PA conversion film using pneumatic actuation method. The PA film is designed into a circular suspending elastic membran...
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#1Linli Shi (BU: Boston University)H-Index: 1
#2Ying Jiang (BU: Boston University)H-Index: 4
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Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical treatments. Here, we developed a tapered fiber optoacoustic emitter (TFOE) generating an ultrasound field with a high spatial precision of 39.6 µm, enabling optoacoustic activation of single neurons or subcellular structures, such as axons and dendrites. Temporally, a single acoustic pulse of sub-microsecond converted by the TFOE from a s...
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#1Guang YangH-Index: 5
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We demonstrate a novel fiber endface photoacoustic (PA) generator using infrared (IR) 144 laser dye dispersed within an ultraviolet adhesive. The generator provides a wide acoustic bandwidth in the transducer frequency range of 2–7 MHz, high thermal conversion efficiency ({\gt}90\%, good PA signal controllability (well-controlled IR 144 concentration), and high feasibility (simple procedures). Through a series of experimental validations, we show that this fiber-based endface PA generator can...
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#1Ying Jiang (BU: Boston University)H-Index: 4
#2Yimin Huang (BU: Boston University)H-Index: 10
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Summary Neuromodulation is an invaluable approach for the study of neural circuits and clinical treatment of neurological diseases. Here, we report semiconducting polymer nanoparticles based photoacoustic nanotransducers (PANs) for neural stimulation in vitro and in vivo. Our PANs strongly absorb the nanosecond pulsed laser in the near-infrared second window (NIR-II) and generate localized acoustic waves. PANs are shown to be surface modified and selectively bind onto neurons. PAN-mediated activ...
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#1Linli Shi (BU: Boston University)H-Index: 1
#2Ying Jiang (BU: Boston University)H-Index: 4
Last. Chen Yang (BU: Boston University)H-Index: 74
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As an emerging technology, transcranial focused ultrasound has been demonstrated to successfully evoke motor responses in mice, rabbits, and sensory/motor responses in humans. Yet, the spatial resolution of ultrasound does not allow for high-precision stimulation. Here, we developed a tapered fiber optoacoustic emitter (TFOE) for optoacoustic stimulation of neurons with an unprecedented spatial resolution of 20 microns, enabling selective activation of single neurons or subcellular structures, s...
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