Cyasterone accelerates fracture healing by promoting MSCs migration and osteogenesis.

Published on Feb 19, 2021in Journal of orthopaedic translation3.986
· DOI :10.1016/J.JOT.2020.11.004
Jun-Lang Zhu3
Estimated H-index: 3
(Guangzhou University of Chinese Medicine),
Yamei Liu9
Estimated H-index: 9
(Guangzhou University of Chinese Medicine)
+ 11 AuthorsWang Bin4
Estimated H-index: 4
(Guangzhou University of Chinese Medicine)
Source
Abstract
Abstract Background Mesenchymal Stem Cells (MSCs) therapy has become a new coming focus of clinical research in regenerative medicine. However, only a small number of implanted MSCs could successfully reach the injured areas. The previous studies have shown that fracture healing time is inversely proportional to concentration of MSCs in injured tissue. Methods The migration and osteogenesis of MSCs were assessed by transwell assay and Alizarin Red S staining. Levels of gene and protein expression were checked by qPCR and Western Blot. On the other hand, the enhanced migration ability of MSCs induced by Cyasterone was retarded by CXCR4 siRNA. In addition, the rat model of femoral fracture was established to evaluate the effect of Cyasterone on fracture healing. What's more, we also checked the effect of Cyasterone on mobilisation of MSCs in vivo. Results The results showed that Cyasteron increased the number of MSCs in peripheral blood. The concentrations of SDF-1α in serum at different time points were determined by ELISA assay. Micro-CT and histological analysis were used to evaluate the fractured femurs.Our results showed that Cyasterone could promote the migration and osteogenesis capacities of MSCs. The fractured femurs healed faster with treatment of Cyasterone. Meanwhile, Cyasterone could significantly increase the level of SDF-1α in rats with femur fracture. Conclusion Cyasterone could promote migration and osteogenesis of MSCs, and most importantly, it could accelerate bone fracture healing. Translational Potential statement: These findings provide evidence that Cyasterone could be used as a therapeutic reagent for MSCs mobilisation and osteogenesis. What's more, it could acclerate fracture healing.
References34
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#2Liangliang Xu (Guangzhou University of Chinese Medicine)H-Index: 22
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Bone defects caused by bone fractures, malformations, post-operation on tumor and even periodontitis have became serious clinical problems. Although the exact origin of osteoblast precursors is still obscure, mesenchymal stem cells (MSCs) that originate from local bone marrow, periosteum, endosteum, mineralized bone or systemic circulation play key roles in osteoblastic differentiation and secretion of multiple factors during spontaneous healing of bone trauma or defect. Substantial evidences ha...
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Healing and regeneration of bone injuries, particularly those that are associated with large bone defects, are a complicated process. There is growing interest in the application of osteoinductive and osteogenic growth factors and mesenchymal stem cells (MSCs) in order to significantly improve bone repair and regeneration. MSCs are multipotent stromal stem cells that can be harvested from many different sources and differentiated into a variety of cell types, such as preosteogenic chondroblasts ...
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