Wang Bin
Guangzhou University of Chinese Medicine
Regenerative medicinePhosphorylationCXCR4Response elementmicroRNACellular differentiationCellMolecular biologyAlkaline phosphataseReporter geneTranscriptomeAndrologyCalcitriol receptorGene expression profilingChemistryApoptosisProtein kinase CMesenchymal stem cellIn vitroIn vivoSmall interfering RNASuperoxide dismutasePropidium iodideOxidative stressRUNX2Western blotOsteoblastROCK2Homing (hematopoietic)VDREBone marrowFemoral fractureWNT5AWound healingCartilageMTT assayOsteopontinStem cellPopulationTransplantationFemur fractureChondrogenesisReactive oxygen speciesCa2+/calmodulin-dependent protein kinaseCancer researchViability assayTransfectionSignal transductionMedicineMyosin light-chain kinaseBone healingTranscription factorBiologyHedgehog signaling pathwayAnnexinCell biology
Publications 9
#1Jun-Lang Zhu (Guangzhou University of Chinese Medicine)H-Index: 3
#2Yamei Liu (Guangzhou University of Chinese Medicine)H-Index: 9
Last. Wang Bin (Guangzhou University of Chinese Medicine)H-Index: 4
view all 14 authors...
Abstract Background Mesenchymal Stem Cells (MSCs) therapy has become a new coming focus of clinical research in regenerative medicine. However, only a small number of implanted MSCs could successfully reach the injured areas. The previous studies have shown that fracture healing time is inversely proportional to concentration of MSCs in injured tissue. Methods The migration and osteogenesis of MSCs were assessed by transwell assay and Alizarin Red S staining. Levels of gene and protein expressio...
#1Yamei Liu (Guangzhou University of Chinese Medicine)H-Index: 9
#2Liangliang Xu (Guangzhou University of Chinese Medicine)H-Index: 22
Last. Gang Li (CUHK: The Chinese University of Hong Kong)H-Index: 84
view all 12 authors...
Abstract Mesenchymal stem cells (MSCs) can be easily expanded without losing the ability of multilineage differentiation, including oesteogenic, chondrogenic and adipogenic differentiation. These characters make MSCs a promising cell resource for cartilage defect repair. MSCs could be recruited by inflammatory stimulation, then home to the injury tissues. However, its capacity of homing is extremely limited. Thus, it has become extremely necessary to develop an agent or a method, which can be us...
1 CitationsSource
#1Yamei Liu (Guangzhou University of Chinese Medicine)H-Index: 9
#2Wang-Yang Li (Hunan University)H-Index: 1
Last. Wang Bin (GU: Guangzhou University)H-Index: 4
view all 14 authors...
ABSTRACT MSC transplantation has been explored as a new clinical approach to stem cell-based therapies for bone diseases in regenerative medicine due to their osteogenic capability. However, only a small population of implanted MSC could successfully reach the injured areas. Therefore, enhancing MSC migration could be a beneficial strategy to improve the therapeutic potential of cell transplantation. Catharmus tinctorius volatile oil (CTVO) was found to facilitate MSC migration. Further explorat...
2 CitationsSource
#1Liuchao Hu (Guangzhou University of Chinese Medicine)H-Index: 3
#1Liuchao Hu (Guangzhou University of Chinese Medicine)H-Index: 1
Last. Yiwen Luo (Guangzhou University of Chinese Medicine)H-Index: 3
view all 13 authors...
: Bone fractures are very common, and above 5% of the fractures are impaired, leading to nonunions and severe disablilities. The traditional Chinese medicine Bushen Huoxue decoction (BHD) has been used to treat fracture in China. Our previous report has found that BHD promotes migration of rat mesenchymal stem cells (rMSCs) by activating Wnt5a signaling pathway. However, whether and how miRNAs are involved in modulating rMSCs migration induced by BHD has not been explored. In the present study, ...
2 CitationsSource
#1Qiuke Hou (Guangzhou University of Chinese Medicine)H-Index: 7
#2Yongquan Huang (Guangzhou University of Chinese Medicine)H-Index: 6
Last. Dongfeng Chen (Guangzhou University of Chinese Medicine)H-Index: 22
view all 9 authors...
Our previous study showed that (+)-cholesten-3-one (CN) has the potential to induce the osteoblastic differentiation of mesenchymal stem cells (MSCs). However, the roles of CN in targeting miRNA-mRNA-lncRNA interactions to regulate osteoblast differentiation remain poorly understood. A total of 77 miRNAs (36 upregulated and 41 downregulated) and 295 lncRNAs (281 upregulated and 14 downregulated) were significantly differentially expressed during CN-induced MSC osteogenic differentiation. Bioinfo...
9 CitationsSource
#1Wei Shen (GU: Guangzhou University)H-Index: 2
#2Hui Luo (GU: Guangzhou University)H-Index: 1
Last. Yiwen Luo (GU: Guangzhou University)H-Index: 5
view all 10 authors...
Background Bushen Huoxue decoction (BHD) has a significant effect on fracture rehabilitation, yet its underlying mechanism is still unknown. The purpose of this study was to explore whether BHD promotes bone marrow mesenchymal stem cell (BMSC) migration through the Wnt5a signalling pathway.
5 CitationsSource
#1Qiuke Hou (GU: Guangzhou University)H-Index: 3
#2Yong-Quan Huang (GU: Guangzhou University)H-Index: 2
Last. Dong-Feng Chen (GU: Guangzhou University)H-Index: 6
view all 9 authors...
Our previous reports indicated that (+)-cholesten-3-one induces osteogenic differentiation of bone marrow mesenchymal stem cells (MSCs) by activating vitamin D receptor (VDR). However, whether and how miRNAs modulate osteogenic differentiation induced by (+)-cholesten-3-one have not been explored. In this study, miRNA array profiling and further validation by quantitative real-time PCR revealed that miR-351 was downregulated during (+)-cholesten-3-one-induced osteogenic differentiation of MSCs. ...
5 Citations
#1Qiu‑Ke Hou (GU: Guangzhou University)H-Index: 1
#2Yong‑Quan Huang (GU: Guangzhou University)H-Index: 1
Last. Dong-Feng Chen (GU: Guangzhou University)H-Index: 6
view all 10 authors...
: In our previous reports, it was revealed that steroids in traditional Chinese medicine (TCM) have the therapeutic potential to treat bone disease. In the present study, an in vitro model of a vitamin D receptor response element (VDRE) reporter gene assay in mesenchymal stem cells (MSCs) was used to identify steroids that enhanced osteogenic differentiation of MSCs. (+)-cholesten-3-one (CN), which possesses a ketone group that is modified in cholesterol and cholesterol myristate, effectively pr...
2 CitationsSource
#1Wangyang Li (GU: Guangzhou University)H-Index: 1
#2Yamei Liu (GU: Guangzhou University)H-Index: 9
Last. Yunpu Xiong (GU: Guangzhou University)H-Index: 2
view all 8 authors...
: Bone marrow-derived mesenchymal stem cells (BMSCs) have the potential to be used for the treatment of delayed union, nonunion or persistent bone defects in MSC-based cell therapy. However, implantation of BMSCs into the fracture site is confronted with apoptosis on account of harsh conditions and oxidative stress. In the present study, the anti-apoptotic effects of berberine (BBR) on BMSCs subjected to hydrogen peroxide (H2O2) are investigated, and the potential underlying mechanisms are explo...
13 CitationsSource