miRNA-205 Nanoformulation Sensitizes Prostate Cancer Cells to Chemotherapy

Published on Aug 25, 2018in Cancers6.126
· DOI :10.3390/CANCERS10090289
Prashanth K.B. Nagesh10
Estimated H-index: 10
(UTHSC: University of Tennessee Health Science Center),
Pallabita Chowdhury12
Estimated H-index: 12
(UTHSC: University of Tennessee Health Science Center)
+ 7 AuthorsMurali M. Yallapu34
Estimated H-index: 34
(UTHSC: University of Tennessee Health Science Center)
Sources
Abstract
: The therapeutic application of microRNA(s) in the field of cancer has generated significant attention in research. Previous studies have shown that miR-205 negatively regulates prostate cancer cell proliferation, metastasis, and drug resistance. However, the delivery of miR-205 is an unmet clinical need. Thus, the development of a viable nanoparticle platform to deliver miR-205 is highly sought. A novel magnetic nanoparticle (MNP)-based nanoplatform composed of an iron oxide core with poly(ethyleneimine)-poly(ethylene glycol) layer(s) was developed. An optimized nanoplatform composition was confirmed by examining the binding profiles of MNPs with miR-205 using agarose gel and fluorescence methods. The novel formulation was applied to prostate cancer cells for evaluating cellular uptake, miR-205 delivery, and anticancer, antimetastasis, and chemosensitization potentials against docetaxel treatment. The improved uptake and efficacy of formulations were studied with confocal imaging, flow cytometry, proliferation, clonogenicity, Western blot, q-RT-PCR, and chemosensitization assays. Our findings demonstrated that the miR-205 nanoplatform induces significant apoptosis and enhancing chemotherapeutic effects in prostate cancer cells. Overall, these study results provide a strong proof-of-concept for a novel nonviral-based nanoparticle protocol for effective microRNA delivery to prostate cancer cells.
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