Non-genetic photoacoustic stimulation of single neurons by a tapered fiber optoacoustic emitter.

Published on Jul 14, 2021in Light-Science & Applications17.782
· DOI :10.1038/S41377-021-00580-Z
Linli Shi1
Estimated H-index: 1
(BU: Boston University),
Ying Jiang4
Estimated H-index: 4
(BU: Boston University)
+ 6 AuthorsChen Yang74
Estimated H-index: 74
(BU: Boston University)
Sources
Abstract
Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical treatments. Here, we developed a tapered fiber optoacoustic emitter (TFOE) generating an ultrasound field with a high spatial precision of 39.6 µm, enabling optoacoustic activation of single neurons or subcellular structures, such as axons and dendrites. Temporally, a single acoustic pulse of sub-microsecond converted by the TFOE from a single laser pulse of 3 ns is shown as the shortest acoustic stimuli so far for successful neuron activation. The precise ultrasound generated by the TFOE enabled the integration of the optoacoustic stimulation with highly stable patch-clamp recording on single neurons. Direct measurements of the electrical response of single neurons to acoustic stimulation, which is difficult for conventional ultrasound stimulation, have been demonstrated. By coupling TFOE with ex vivo brain slice electrophysiology, we unveil cell-type-specific responses of excitatory and inhibitory neurons to acoustic stimulation. These results demonstrate that TFOE is a non-genetic single-cell and sub-cellular modulation technology, which could shed new insights into the mechanism of ultrasound neurostimulation. A tapered fiber optoacoustic emitter enables non-genetic single neuron and sub-cellular stimulation. It also offers high compatibility with stable patch clamp recording for further study of mechanism.
References58
Newest
#1Linli Shi (BU: Boston University)H-Index: 1
#2Ying Jiang (BU: Boston University)H-Index: 4
Last. Chen Yang (BU: Boston University)H-Index: 74
view all 7 authors...
Abstract Focused ultrasound has attracted great attention in minimally invasive therapeutic and mechanism studies. Frequency below 1 MHz is identified preferable for high-efficiency bio-modulation. However, the poor spatial confinement of several millimeters and large device diameter of ∼25 mm of typical sub-MHz ultrasound technology suffered from the diffraction limit, severely hindering its further applications. To address it, a fiber-based optoacoustic emitter (FOE) is developed, serving as a...
Source
#1Ying Jiang (BU: Boston University)H-Index: 4
#2Hyeon Jeong LeeH-Index: 11
Last. Ji-Xin ChengH-Index: 84
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Low-intensity ultrasound is an emerging modality for neuromodulation. Yet, transcranial neuromodulation using low-frequency piezo-based transducers offers poor spatial confinement of excitation volume, often bigger than a few millimeters in diameter. In addition, the bulky size limits their implementation in a wearable setting and prevents integration with other experimental modalities. Here, we report spatially confined optoacoustic neural stimulation through a miniaturized Fiber-Optoacoustic C...
Source
Neuronal activity can be modulated by mechanical stimuli. To study this phenomenon quantitatively, we mechanically stimulated rat cortical neurons by shear stress and local indentation. Neurons show 2 distinct responses, classified as transient and sustained. Transient responses display fast kinetics, similar to spontaneous neuronal activity, whereas sustained responses last several minutes before returning to baseline. Local soma stimulations with micrometer-sized beads evoke transient response...
Source
#1Hod Dana (Cleveland Clinic)H-Index: 17
#2Yi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 15
Last. Douglas S. Kim (HHMI: Howard Hughes Medical Institute)H-Index: 24
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Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, ...
Source
#1Y Chen (University of Tübingen)H-Index: 4
#2Patricia Pais-Roldán (University of Tübingen)H-Index: 6
Last. Xin Yu (MPG: Max Planck Society)H-Index: 63
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Optical fiber-mediated optogenetic activation and neuronal Ca2+ recording in combination with fMRI provide a multi-modal fMRI platform. Here, we developed an MRI-guided robotic arm (MgRA) as a flexible positioning system with high precision to real-time assist optical fiber brain intervention for multi-modal animal fMRI. Besides the ex vivo precision evaluation, we present the highly reliable brain activity patterns in the projected basal forebrain regions upon MgRA-driven optogenetic stimulatio...
Source
#1Joseph Blackmore (University of Oxford)H-Index: 3
#2Shamit Shrivastava (University of Oxford)H-Index: 12
Last. Robin O. Cleveland (University of Oxford)H-Index: 35
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Abstract Ultrasonic neuromodulation is a rapidly growing field, in which low-intensity ultrasound (US) is delivered to nervous system tissue, resulting in transient modulation of neural activity. This review summarizes the findings in the central and peripheral nervous systems from mechanistic studies in cell culture to cognitive behavioral studies in humans. The mechanisms by which US mechanically interacts with neurons and could affect firing are presented. An in-depth safety assessment of cur...
Source
#1Augix Guohua Xu (ZJU: Zhejiang University)H-Index: 4
#2Meizhen Qian (ZJU: Zhejiang University)H-Index: 3
Last. Anna W. RoeH-Index: 41
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We have developed a way to map brain-wide networks using focal pulsed infrared neural stimulation in ultrahigh-field magnetic resonance imaging (MRI). The patterns of connections revealed are similar to those of connections previously mapped with anatomical tract tracing methods. These include connections between cortex and subcortical locations and long-range cortico-cortical connections. Studies of local cortical connections reveal columnar-sized laminar activation, consistent with feed-forwar...
Source
#1Cosimo Prestigio (IIT: Istituto Italiano di Tecnologia)H-Index: 5
#2Daniele Ferrante (UniGe: University of Genoa)H-Index: 3
Last. Pietro Baldelli (UniGe: University of Genoa)H-Index: 40
view all 8 authors...
Cultured hippocampal neurons represent the most widely used experimental substrate for in vitro electrophysiological studies. Nevertheless, in most cases, the nature of neuron under study is not identified as excitatory or inhibitory, or even worse, recorded neurons are considered as excitatory because of the paucity of GABAergic interneurons. Thus, the definition of reliable criteria able to guarantee an unequivocal identification of excitatory and inhibitory cultured hippocampal neurons is an ...
Source
#1Christian Ineichen (UZH: University of Zurich)H-Index: 9
Last. Oǧuzkan SürücüH-Index: 1
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Deep brain stimulation (DBS) has been proven to be an effective treatment modality for various late-stage neurological and psychiatric disorders. However, knowledge on the electrical field distribution in the brain tissue is still scarce. Uptodate,Most recent attempts to e perspective of understand ding electric field spread was were primarily focused on the effect of different electrodes on rather simple tissue models. The influence of microanatomic, biophysical tissue properties in particular ...
Source
#1Hod Dana (HHMI: Howard Hughes Medical Institute)H-Index: 17
#2Yi Sun (HHMI: Howard Hughes Medical Institute)H-Index: 15
Last. Douglas S. Kim (HHMI: Howard Hughes Medical Institute)H-Index: 24
view all 17 authors...
Calcium imaging with genetically encoded calcium indicators (GECIs) is routinely used to measure neural activity in intact nervous systems. GECIs are frequently used in one of two different modes: to track activity in large populations of neuronal cell bodies, or to follow dynamics in subcellular compartments such as axons, dendrites and individual synaptic compartments. Despite major advances, calcium imaging is still limited by the biophysical properties of existing GECIs, including affinity, ...
Source
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