Comparative Transcriptomics of Shiga Toxin-Producing and Commensal Escherichia coli and Cytokine Responses in Colonic Epithelial Cell Culture Infections.

Published on Oct 26, 2020in Frontiers in Cellular and Infection Microbiology5.293
· DOI :10.3389/FCIMB.2020.575630
Lisa M. Harrison4
Estimated H-index: 4
(CFSAN: Center for Food Safety and Applied Nutrition),
David W. Lacher25
Estimated H-index: 25
(CFSAN: Center for Food Safety and Applied Nutrition)
+ 1 AuthorsSusan R. Leonard7
Estimated H-index: 7
(CFSAN: Center for Food Safety and Applied Nutrition)
Ingestion of Shiga toxin-producing Escherichia coli (STEC) can result in a range of illness severity from asymptomatic to hemorrhagic colitis and death; thus risk assessment of STEC strains for human pathogenicity is important in the area of food safety. Illness severity depends in part on the combination of virulence genes carried in the genome, which can vary between strains even of identical serotype. To better understand how core genes are regulated differently among strains and to identify possible novel STEC virulence gene candidates that could be added to the risk assessment repertoire, we used comparative transcriptomics to investigate global gene expression differences between two STEC strains associated with severe illness and a commensal E. coli strain during in vitro intestinal epithelial cell (IEC) infections. Additionally, we compared a wide array of concomitant cytokine levels produced by the IECs. The cytokine expression levels were examined for a pattern representing STEC pathogenicity; however, while one STEC strain appeared to elicit a proinflammatory response, infection by the other strain produced a pattern comparable to the commensal E. coli. This result may be explained by the significant differences in gene content and expression observed between the STEC strains. RNA-Seq analysis revealed considerable disparity in expression of genes in the arginine and tryptophan biosynthesis/import pathways between the STEC strains and the commensal E. coli strain, highlighting the important role some amino acids play in STEC colonization and survival. Contrasting differential expression patterns were observed for genes involved in respiration among the three strains suggesting that metabolic diversity is a strategy utilized to compete with resident microflora for successful colonization. Similar temporal expression results for known and putative virulence genes were observed in the STEC strains, revealing strategies used for survival prior to and after initial adherence to IECs. Additionally, three genes encoding hypothetical proteins located in mobile genetic elements were, after interrogation of a large set of E. coli genomes, determined to likely represent novel STEC virulence factors.
#1Lin Chen (NUS: National University of Singapore)H-Index: 21
#2Xue Zhao (NUS: National University of Singapore)H-Index: 16
Last. Hongshun Yang (NUS: National University of Singapore)H-Index: 50
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Abstract The metabolic diversity of Escherichia coli strains (non-pathogenic E. coli ATCC 25922, and pathogenic E. coli O157:H7, O26:H11, O45:H2, O103:H11, O111, O121:H19, and O145) was tested using nuclear magnetic resonance. Based on two representative two-dimensional 1H-13C spectra, 38 metabolites were identified in E. coli intracellular samples. Principal component analysis indicated that metabolites including lysine, arginine, α-ketoglutaric acid, adenosine, and fumaric acid were responsibl...
#1Hiroaki Baba (Tohoku University)H-Index: 3
#2Hajime Kanamori (Tohoku University)H-Index: 23
Last. Mitsuo Kaku (Tohoku University)H-Index: 34
view all 12 authors...
Shiga toxin-producing Escherichia coli (STEC) can cause severe gastrointestinal disease and colonization among food handlers. In Japan, STEC infection is a notifiable disease, and food handlers are required to undergo routine stool examination for STEC. However, the molecular epidemiology of STEC is not entirely known. We investigated the genomic characteristics of STEC from patients and asymptomatic food handlers in Miyagi Prefecture, Japan. Whole-genome sequencing (WGS) was performed on 65 STE...
#1Daniel Charlier (Vrije Universiteit Brussel)H-Index: 31
#2Indra Bervoets (Vrije Universiteit Brussel)H-Index: 7
Already very early, the study of microbial arginine biosynthesis and its regulation contributed significantly to the development of new ideas and concepts. Hence, the term “repression” was proposed by Vogel (The chemical basis of heredity, The John Hopkins Press, Baltimore, 1957) (in opposition to induction) to describe the relative decrease in acetylornithinase production in Escherichia coli cells upon arginine supplementation, whereas the term “regulon” was coined by Maas and Clark (J Mol Biol...
#1Aman Kumar (UTSW: University of Texas Southwestern Medical Center)H-Index: 7
#2Vanessa Sperandio (UTSW: University of Texas Southwestern Medical Center)H-Index: 63
ABSTRACT Microbial establishment within the gastrointestinal (GI) tract requires surveillance of the gut biogeography. The gut microbiota coordinates behaviors by sensing host- or microbiota-derived signals. Here we show for the first time that microbiota-derived indole is highly prevalent in the lumen compared to the intestinal tissue. This difference in indole concentration plays a key role in modulating virulence gene expression of the enteric pathogens enterohemorrhagic Escherichia coli (EHE...
#1Petya Berger (WWU: University of Münster)H-Index: 8
#2Ivan U. Kouzel (WWU: University of Münster)H-Index: 10
Last. Alexander Mellmann (WWU: University of Münster)H-Index: 63
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Background Enterohemorrhagic Escherichia coli (E. coli) are intestinal pathogenic bacteria that cause life-threatening disease in humans. Their cardinal virulence factor is Shiga toxin (Stx), which is encoded on lambdoid phages integrated in the chromosome. Stx phages can infect and lysogenize susceptible bacteria, thus either increasing the virulence of already pathogenic bacterial hosts or transforming commensal strains into potential pathogens. There is increasing evidence that Stx phage-enco...
#1Narjol Gonzalez-Escalona (CFSAN: Center for Food Safety and Applied Nutrition)H-Index: 28
#2Julie A. Kase (CFSAN: Center for Food Safety and Applied Nutrition)H-Index: 13
Illnesses caused by Shiga toxin-producing Escherichia coli (STECs) can be life threatening, such as hemolytic uremic syndrome (HUS). The STECs most frequently identified by USDA’s Microbiological Data Program (MDP) carried toxin gene subtypes stx1a and/or stx2a. Here we described the genome sequences of 331 STECs isolated from foods regulated by the FDA 2010–2017, and determined their genomic identity, serotype, sequence type, virulence potential, and prevalence of antimicrobial resistance. Isol...
#1Melissa D Barnes (Case Western Reserve University)H-Index: 10
#2Vijay Kumar (Case Western Reserve University)H-Index: 8
Last. Robert A. BonomoH-Index: 85
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ABSTRACT Multidrug-resistant (MDR) Acinetobacter spp. poses a significant therapeutic challenge in part due to the presence of chromosomally encoded β-lactamases, including class C Acinetobacter-derived cephalosporinases (ADC) and class D oxacillinases (OXA), as well as plasmid-mediated class A β-lactamases. Importantly, OXA-like β-lactamases represent a gap in the spectrum of inhibition by recently approved β-lactamase inhibitors such as avibactam and vaborbactam. ETX2514 is a novel, rationally...
#1Jiale Ma (NAU: Nanjing Agricultural University)H-Index: 1
#2Chunxia An (NAU: Nanjing Agricultural University)H-Index: 2
Last. Ganwu Li (Harbin Veterinary Research Institute)H-Index: 24
view all 7 authors...
: Extraintestinal pathogenic Escherichia coli (ExPEC) is one of the leading causes of bloodstream infections. Characteristically, these organisms exhibit strong resistance to the bactericidal action of host serum. Although numerous serum resistance factors in ExPEC have been identified, their regulatory mechanisms during in vivo infection remain largely unknown. Here, RNA sequencing analyses together with quantitative reverse-transcription PCR revealed that ExPEC genes involved in the biosynthes...
#1Kazuei Igarashi (Chiba University)H-Index: 82
#2Keiko Kashiwagi (Chiba Institute of Science)H-Index: 58
: The polyamines (PA) putrescine, spermidine, and spermine have numerous roles in the growth of both prokaryotic and eukaryotic cells. For example, it is well known that putrescine and spermidine are strongly involved in proliferation and viability of Escherichia coli cells. Studies of polyamine functions and distributions in E. coli cells have revealed that polyamines mainly exist as an RNA-polyamine complex. Polyamines stimulate the assembly of 30S ribosomal subunits and thereby increase gener...
Cited By1
#2Yu-Jin Jeong (Korea Research Institute of Bioscience and Biotechnology)H-Index: 4
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