Engineering Multi‐Cellular Spheroids for Tissue Engineering and Regenerative Medicine

Multi‐cellular spheroids are formed as a 3D structure with dense cell–cell/cell–extracellular matrix interactions, and thus, have been widely utilized as implantable therapeutics and various ex vivo tissue models in tissue engineering. In principle, spheroid culture methods maximize cell–cell cohesion and induce spontaneous cellular assembly while minimizing cellular interactions with substrates by using physical forces such as gravitational or...