Heungsoo Shin
Hanyang University
Self-healing hydrogelsSurface modificationBiophysicsBiomedical engineeringElectrospinningCellNanotechnologyChemistryMaterials scienceMesenchymal stem cellBiomaterialStem cellTransplantationBone regenerationChemical engineeringTissue engineeringAdhesionCell adhesionNanofiberCell biology
140Publications
29H-index
2,948Citations
Publications 142
Newest
#1Jin-Kyu Lee (Hanyang University)H-Index: 21
#2Sangmin Lee (Hanyang University)H-Index: 6
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 4 authors...
BACKGROUND Fabrication of three-dimensional stem cell spheroids have been studied to improve stem cell function, but the hypoxic core and limited penetration of nutrients and signaling cues to the interior of the spheroid were challenges. The incorporation of polymers such as silica and gelatin in spheroids resulted in relatively relaxed assembly of composite spheroids, and enhancing transport of nutrient and biological gas. However, because of the low surface area between cells and since the po...
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#1Se jeong Kim (Hanyang University)H-Index: 5
#2Sangmin Lee (Hanyang University)H-Index: 6
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 4 authors...
Abstract Techniques for harvest and delivery of cell sheets have been improving for decades. However, cell sheets with complicated patterns closely related to natural tissue architecture were hardly achieved. Here, we developed an efficient method to culture and harvest cell sheets with complex shape (noted as microtissues) using temperature-responsive hydrogel consisting of expandable polyethylene oxide polymer at low temperature. Firstly, a temperature-responsive hydrogel surface with honeycom...
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#1Sangmin Lee (Hanyang University)H-Index: 6
#2Jin-Kyu Lee (Hanyang University)H-Index: 21
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
Abstract In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes of diminishing therapeutic effects. In this study, we prepared biomaterial surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, and evaluated their anti-oxidative and ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity and anti-oxidative ...
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#1Ha Yeon Byun (Hanyang University)
#2Gyu Nam Jang (Hanyang University)
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 6 authors...
Stem cell spheroids have been widely investigated to accelerate bone tissue regeneartion. However, the directed differentiation of stem cells into osteoblastic lineage and the prevention of cells from damage by reactive oxygen species (ROS) remain challenge. Here, we developed osteoinductive and ROS scavenging extracellular matrix (ECM)-mimicking synthetic fibers based on epigallocatechin gallate (EGCG) coating. They were then utilized to fabricate engineered spheroids with human adipose-derived...
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#2John Fisher (UMD: University of Maryland, College Park)H-Index: 82
Last. Xiumei Wang (THU: Tsinghua University)H-Index: 23
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#1Sangmin Lee (Hanyang University)H-Index: 6
#2Jin-Kyu Lee (Hanyang University)H-Index: 21
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes of diminishing therapeutic effects. In this study, we prepared biomaterial surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, and evaluated their anti-oxidative and ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity and anti-oxidative propertie...
Source
#1Jennifer J. Lee (SNU: Seoul National University)H-Index: 2
#2Se jeong Kim (Hanyang University)H-Index: 5
Last. Seong Keun Kwon (Seoul National University Hospital)H-Index: 16
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Abstract The goal of this study was to investigate the effect of transplantation of an adipose-derived stem cell (ASC) spheroid sheet on the regeneration of ulcerated oral mucosa. The ASC spheroid sheet was constructed by seeding three-dimensional (3D) spheroidal ASCs on a temperature-responsive hydrogel, where cell adhesion and spreading were improved by incorporating fibronectin. The 3D culture of ASCs enhanced their inherent paracrine properties for up to 1 week after transferring to two-dime...
1 CitationsSource
#1Jin-Kyu Lee (Hanyang University)H-Index: 21
#2Ji Min SeokH-Index: 6
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
Three-dimensional (3D)-printed scaffolds have proved to be effective tools for delivering growth factors and cells in bone-tissue engineering. However, delivering spheroids that enhance cellular function remains challenging because the spheroids tend to suffer from low viability, which limits bone regenerationin vivo. Here, we describe a 3D-printed polycaprolactone micro-chamber that can deliver human adipose-derived stem cell spheroids. Anin vitroculture of cells from spheroids in the micro-cha...
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#1Jin-Kyu Lee (Hanyang University)H-Index: 21
#2Sangmin Lee (Hanyang University)H-Index: 6
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
Abstract Stem cells with mineralized materials have been used for bone regeneration; however, engineering the complex vascularized structure of the natural bone remains a challenge. Here, we developed platelet-derived growth factor (PDGF) and bio-mineral coated fibers which were then assembled with human adipose-derived stem cells (hADSCs) to form spheroids as building blocks for vascularized bone regeneration. The PDGF incorporated within the spheroid increased the proliferation of hADSCs, whic...
4 CitationsSource
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