Se jeong Kim
Hanyang University
Self-healing hydrogelsSurface modificationBiophysicsBiomedical engineering3D cell cultureNanotechnologySpheroidChemistryMaterials scienceMesenchymal stem cellEpigallocatechin gallateBiomaterialStem cellTransplantationReactive oxygen speciesTissue engineeringMicrocontact printingCell cultureNanofiberCell biology
13Publications
5H-index
126Citations
Publications 13
Newest
#1Se jeong Kim (Hanyang University)H-Index: 5
#2Sangmin Lee (Hanyang University)H-Index: 6
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 4 authors...
Abstract Techniques for harvest and delivery of cell sheets have been improving for decades. However, cell sheets with complicated patterns closely related to natural tissue architecture were hardly achieved. Here, we developed an efficient method to culture and harvest cell sheets with complex shape (noted as microtissues) using temperature-responsive hydrogel consisting of expandable polyethylene oxide polymer at low temperature. Firstly, a temperature-responsive hydrogel surface with honeycom...
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#1Sangmin Lee (Hanyang University)H-Index: 6
#2Jin-Kyu Lee (Hanyang University)H-Index: 21
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
Abstract In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes of diminishing therapeutic effects. In this study, we prepared biomaterial surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, and evaluated their anti-oxidative and ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity and anti-oxidative ...
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#1Sangmin Lee (Hanyang University)H-Index: 6
#2Jin-Kyu Lee (Hanyang University)H-Index: 21
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 7 authors...
In tissue engineering, excessively generated reactive oxygen species (ROS) during biomaterial implantation or cell transplantation is a one of major causes of diminishing therapeutic effects. In this study, we prepared biomaterial surfaces coated with antioxidant epigallocatechin gallate (EGCG) and metal ions, and evaluated their anti-oxidative and ROS scavenging properties. We revealed that EGCG-coating on polycaprolactone (PCL) film surface increased hydrophilicity and anti-oxidative propertie...
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#1Jennifer J. Lee (SNU: Seoul National University)H-Index: 2
#2Se jeong Kim (Hanyang University)H-Index: 5
Last. Seong Keun Kwon (Seoul National University Hospital)H-Index: 16
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Abstract The goal of this study was to investigate the effect of transplantation of an adipose-derived stem cell (ASC) spheroid sheet on the regeneration of ulcerated oral mucosa. The ASC spheroid sheet was constructed by seeding three-dimensional (3D) spheroidal ASCs on a temperature-responsive hydrogel, where cell adhesion and spreading were improved by incorporating fibronectin. The 3D culture of ASCs enhanced their inherent paracrine properties for up to 1 week after transferring to two-dime...
1 CitationsSource
#1Se jeong Kim (Hanyang University)H-Index: 5
#2Eun Mi Kim (Hanyang University)H-Index: 12
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 5 authors...
Multi-cellular spheroids are formed as a 3D structure with dense cell-cell/cell-extracellular matrix interactions, and thus, have been widely utilized as implantable therapeutics and various ex vivo tissue models in tissue engineering. In principle, spheroid culture methods maximize cell-cell cohesion and induce spontaneous cellular assembly while minimizing cellular interactions with substrates by using physical forces such as gravitational or centrifugal forces, protein-repellant biomaterials,...
5 CitationsSource
#1Hawon Lee (Hanyang University)H-Index: 2
#2Se jeong Kim (Hanyang University)H-Index: 5
Last. Young-Pil Kim (Hanyang University)H-Index: 24
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Despite the diverse roles of cell-secreted proteases in the extracellular matrix (ECM), classical methods to analyze protease activity have not been explored at the cell culture site. Here we report a stable, matrix-sticky, and protease-sensitive extracellular reporter that comprises a collagen-binding protein and a Forster resonance energy transfer (FRET) coupler of enhanced green fluorescent protein and small dye molecule. The extracellular FRET reporter via split intein-mediated protein trans...
2 CitationsSource
#1Eun Mi Kim (Hanyang University)H-Index: 12
#2Yu Bin Lee (Hanyang University)H-Index: 15
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 8 authors...
Abstract Cell spheroids as building blocks for engineering micro-tissue should be able to mimic the complex structure of natural tissue. However, control of the distribution of multiple cell populations within cell spheroids is difficult to achieve with current spheroid-harvest methods such as hanging-drop and with the use of microwell plates. In this study, we report the fabrication of core-shell spheroids with the ultimate goal to form 3D complex micro-tissue. We used endothelial cells and two...
9 CitationsSource
#1Se jeong Kim (Hanyang University)H-Index: 5
#2Jaesung Park (Hanyang University)H-Index: 3
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 9 authors...
Abstract 3D culture of stem cells can improve therapeutic effects. However, there is limited research on how to deliver cultured stem cell spheroids to the desired target. Here, we developed lotus seedpod-inspired hydrogel (LoSH) containing microwells for culture and delivery of stem cell spheroids. Human adipose-derived stem cells (hADSCs) inside the square microwells (200 or 400 μm in width with various depths) spontaneously formed spheroids with high viability (94.08 ± 1.56%), and fibronectin...
5 CitationsSource
#1Sorour Nemati (Hanyang University)H-Index: 5
#2Se jeong Kim (Hanyang University)H-Index: 5
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 4 authors...
Tissue engineering uses a combination of cell biology, chemistry, and biomaterials to fabricate three dimensional (3D) tissues that mimic the architecture of extracellular matrix (ECM) comprising diverse interwoven nanofibrous structure. Among several methods for producing nanofibrous scaffolds, electrospinning has gained intense interest because it can make nanofibers with a porous structure and high specific surface area. The processing and solution parameters of electrospinning can considerab...
34 CitationsSource
#1Se jeong Kim (Hanyang University)H-Index: 5
#2Jaesung Park (Hanyang University)H-Index: 3
Last. Heungsoo Shin (Hanyang University)H-Index: 29
view all 8 authors...
Abstract Stem cell spheroids have been studied extensively in organoid culture and therapeutic transplantation. Herein, hydrogels with an embossed surface (HES) were developed as an all-in-one platform that can enable the rapid formation and culture of a large quantity of size-controllable stem cell spheroids. The embossed structure on the hydrogel was adjustable according to the grit designation of the sandpaper. Human adipose-derived stem cells (hADSCs) were rapidly assembled into spheroids on...
18 CitationsSource