Fingerprint Spectroscopic SRS Imaging of Single Living Cells and Whole Brain by Ultrafast Tuning and Spatial-Spectral Learning

Label-free vibrational imaging by stimulated Raman scattering (SRS) provides unprecedented insight into real-time chemical distributions in living systems. Specifically, SRS in the fingerprint region can resolve multiple chemicals in a complex bio-environment using specific and well-separated Raman signatures. Yet, fingerprint SRS imaging with microsecond spectral acquisition has not been achieved due to the small fingerprint Raman...