Ji-Xin Cheng
Boston University
Analytical chemistryOpticsBiophysicsInternal medicineEndocrinologyBiomedical engineeringMicroscopyRaman scatteringNanotechnologyPhotothermal therapyChemistryMaterials scienceLipid dropletRaman spectroscopyOptoelectronicsLaserChemical imagingMicroscopeMedicineBiology
459Publications
83H-index
18.5kCitations
Publications 429
Newest
#2Haonan ZongH-Index: 2
Last. M. Selim ÜnlüH-Index: 3
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Interferometric scattering microscopy has been a very promising technology for highly sensitive label-free imaging of a broad spectrum of biological nanoparticles from proteins to viruses in a high-throughput manner. Although it can reveal the specimen's size and shape information, the chemical composition is inaccessible in interferometric measurements. Infrared spectroscopic imaging provides chemical specificity based on inherent chemical bond vibrations of specimens but lacks the ability to i...
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#1Hongli NiH-Index: 1
Last. Ji-Xin ChengH-Index: 83
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Spectroscopic stimulated Raman scattering (SRS) imaging has become a useful tool finding a broad range of applications. Yet, wider adoption is hindered by the bulky and environmentally-sensitive solid-state optical parametric oscillator (OPO) in current SRS microscope. Moreover, chemically-informative multi-window SRS imaging across C-H, C-D and fingerprint Raman regions is challenging due to the slow wavelength tuning speed of the solid-state OPO. In this work, we present a multi-window SRS ima...
#1Nathan Tague (BU: Boston University)H-Index: 4
#2Haonan Lin (BU: Boston University)H-Index: 6
Last. Mary J. Dunlop (BU: Boston University)H-Index: 19
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Engineered microbes can produce valuable chemicals, however production strains often require extensive optimization before they can be used at industrial scales. To quantify production, metabolic engineers typically employ mass spectrometry, which offers excellent chemical specificity. However, with this approach samples are derived from bulk cultures, obscuring potential cell-to-cell differences in production, and precluding selection methods that rely on measurements of a single cell9s phenoty...
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#1Xiaowei Ge (BU: Boston University)
#2Fátima C. Pereira (University of Vienna)H-Index: 11
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
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One of the biggest challenges in microbiome research in environmental and medical samples is to better understand functional properties of microbial community members at a single cell level. Single cell isotope probing has become a key tool for this purpose, but the currently applied detection methods for measuring isotope incorporation into single cells do not allow high-throughput analyses. Here, we report on the development of an imaging-based approach termed stimulated Raman scattering - two...
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#1Yi Zhang (BU: Boston University)
#1Yi Zhang (BU: Boston University)H-Index: 4
Last. Ji-Xin ChengH-Index: 83
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Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at submicrometer spatial resolution and enables infrared spectroscopy and imaging of living cells and organisms. Yet, current mid-infrared photothermal imaging sensitivity suffers from a weak dependence of scattering on the temperature, and the image quality is vulnerable to...
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#1Linli Shi (BU: Boston University)H-Index: 1
#2Ying Jiang (BU: Boston University)H-Index: 4
Last. Chen Yang (BU: Boston University)H-Index: 69
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Neuromodulation at high spatial resolution poses great significance in advancing fundamental knowledge in the field of neuroscience and offering novel clinical treatments. Here, we developed a tapered fiber optoacoustic emitter (TFOE) generating an ultrasound field with a high spatial precision of 39.6 µm, enabling optoacoustic activation of single neurons or subcellular structures, such as axons and dendrites. Temporally, a single acoustic pulse of sub-microsecond converted by the TFOE from a s...
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#1Pu-Ting Dong (BU: Boston University)H-Index: 6
#2Sebastian Jusuf (BU: Boston University)H-Index: 2
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
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Bacteria have evolved to cope with the detrimental effects of reactive oxygen species (ROS) using their essential molecular components. Catalase, a heme-containing tetramer protein expressed universally in most of the aerobic bacteria, plays an indispensable role in scavenging excess hydrogen peroxide (H2O2). Here, through utilization of wild-type and catalase-deficient mutants, we identified catalase as an endogenous therapeutic target of 400-420 nm blue light. null Catalase residing in bacteri...
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#1Haonan Lin (BU: Boston University)H-Index: 6
#2Hyeon Jeong Lee (ZJU: Zhejiang University)H-Index: 10
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
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Label-free vibrational imaging by stimulated Raman scattering (SRS) provides unprecedented insight into real-time chemical distributions. Specifically, SRS in the fingerprint region (400–1800 cm−1) can resolve multiple chemicals in a complex bio-environment. However, due to the intrinsic weak Raman cross-sections and the lack of ultrafast spectral acquisition schemes with high spectral fidelity, SRS in the fingerprint region is not viable for studying living cells or large-scale tissue samples. ...
3 CitationsSource
#1Juanyong Li (BU: Boston University)H-Index: 2
#1Jing Li (NU: Northwestern University)H-Index: 12
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
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Increased aerobic glycolysis is widely considered as a hallmark of cancer. Yet, cancer cell metabolic reprograming during development of therapeutic resistance is under-studied. Here, through high-throughput stimulated Raman scattering imaging and single cell analysis, we found that cisplatin-resistant cells exhibit increased uptake of exogenous fatty acids, accompanied with decreased glucose uptake and de novo lipogenesis, indicating a reprogramming from glucose and glycolysis dependent to fatt...
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#1Yeran Bai (BU: Boston University)H-Index: 5
#2Jiaze Yin (BU: Boston University)
Last. Ji-Xin Cheng (BU: Boston University)H-Index: 83
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Mid-infrared (IR) spectroscopic imaging using inherent vibrational contrast has been broadly used as a powerful analytical tool for sample identification and characterization. However, the low spatial resolution and large water absorption associated with the long IR wavelengths hinder its applications to study subcellular features in living systems. Recently developed mid-infrared photothermal (MIP) microscopy overcomes these limitations by probing the IR absorption-induced photothermal effect u...
1 CitationsSource