Gene signature

Cell

Gene expression

Mutation

Significance analysis of microarrays

Gene

Temozolomide

Bioinformatics

Biology

Internal medicine

Oncology

Glioma

Biomarker

Genetics

Medicine

IDH1

Gene expression profiling

CD44

Cancer research

EBioMedicine

BackgroundGlioblastoma multiforme (GBM) is a fatal disease without effective therapy. Identification of new biomarkers for prognosis would enable more rational selections of strategies to cure patients with GBM and prevent disease relapse.MethodsSeven datasets derived from GBM patients using microarray or next generation sequencing in R2 online database (http://r2.amc.nl) were extracted and then analyzed using JMP software. The survival distribution was calculated according to the Kaplan-Meier method and the significance was determined using log-rank statistics. The sensitivity of a panel of GBM cell lines in response to temozolomide (TMZ), salinomycin, celastrol, and triptolide treatments was evaluated using MTS and tumor-sphere formation assay.FindingsWe identified that CD44, ATP binding cassette subfamily C member 3 (ABCC3), and tumor necrosis factor receptor subfamily member 1A (TNFRSF1A) as highly expressed genes in GBMs are associated with patients' poor outcomes and therapy resistance. Furthermore, the...

Identification of a panel of genes as a prognostic biomarker for glioblastoma