Transferon™, a peptide mixture with immunomodulatory properties is not immunogenic when administered with various adjuvants

Published on Jul 14, 2017in Journal of Immunotoxicology2.974
· DOI :10.1080/1547691X.2017.1346009
Sandra Avila2
Estimated H-index: 2
(IPN: Instituto Politécnico Nacional),
Leslie Muñoz-García3
Estimated H-index: 3
(IPN: Instituto Politécnico Nacional)
+ 8 AuthorsSonia Mayra Pérez-Tapia11
Estimated H-index: 11
(IPN: Instituto Politécnico Nacional)
Sources
Abstract
AbstractTransferon, a human dialyzable leukocyte extract (hDLE), is a biotherapeutic that comprises a complex mixture of low-molecular-weight peptides (< 10 kDa) and is used to treat diseases with an inflammatory component. Some biotherapeutics, including those composed of peptides, can induce anti-drug antibodies (ADA) that block or diminish their therapeutic effect. Nevertheless, few studies have evaluated peptide-derived drug immunogenicity. In this study, the immunogenicity of Transferon was examined in a murine model during an immunization scheme using the following adjuvants: Al(OH)3, incomplete Freund's adjuvant (IFA), or Titermax Gold. The inoculation scheme entailed three routes of administration (intraperitoneal, Day 1; subcutaneous, Day 7; and intramuscular, Day 14) using 200 μg Transferon/inoculation. Serum samples were collected on Day 21. Total IgG levels were quantitated by affinity chromatography, and specific antibodies against components of Transferon were analyzed by dot-blot and ELISA....
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