Analytical validation of an ultraviolet–visible procedure for determining lutein concentration and application to lutein-loaded nanoparticles

Published on Sep 1, 2017in Food Chemistry6.306
· DOI :10.1016/J.FOODCHEM.2017.03.059
Jéssica Thaís do Prado Silva4
Estimated H-index: 4
(UTFPR: Federal University of Technology - Paraná),
Anderson Clayton da Silva3
Estimated H-index: 3
(UTFPR: Federal University of Technology - Paraná)
+ 7 AuthorsOdinei Hess Gonçalves16
Estimated H-index: 16
(UTFPR: Federal University of Technology - Paraná)
Abstract Lutein is a carotenoid presenting known anti-inflammatory and antioxidant properties. Lutein-rich diets have been associated with neurological improvement as well as reduction of the risk of vision loss due to Age-Related Macular Degeneration (AMD). Micro and nanoencapsulation have demonstrated to be effective techniques in protecting lutein against degradation and also in improving its bioavailability. However, actual lutein concentration inside the capsules and encapsulation efficiency are key parameters that must be precisely known when designing in vitro and in vivo tests. In this work an analytical procedure was validated for the determination of the actual lutein content in zein nanoparticles using ultraviolet–visible spectroscopy. Method validation followed the International Conference on Harmonisation (ICH) guidelines which evaluate linearity, detection limit, quantification limit, accuracy and precision. The validated methodology was applied to characterize lutein-loaded nanoparticles.
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