Transcriptional responses associated with sulfur mustard and thermal burns in porcine skin.

Published on Nov 19, 2008in Cutaneous and Ocular Toxicology1.82
· DOI :10.1080/15569520802092054
James V. Rogers16
Estimated H-index: 16
(Battelle Memorial Institute),
James N. McDougal14
Estimated H-index: 14
(Wright State University)
+ 2 AuthorsJohn S. Graham18
Estimated H-index: 18
Sources
Abstract
In military and civilian environments, serious cutaneous damage can result from thermal burns or exposure to the blistering agent sulfur mustard [bis (2-chloroethyl) sulfide; HD]. Similar therapies have historically been used to treat cutaneous thermal and HD injuries; however, the underlying molecular mechanisms of tissue damage and wound healing may differ between the types of burns. Using microarray analysis, this study assessed the transcriptional responses to cutaneous HD and thermal injury at 48 hours post-exposure to identify molecular networks and genes associated with each type of skin injury. Ventral abdominal sites on each of 4 weanling swine were exposed to 400 μl of undiluted HD or a heated brass rod (70°C) for 8 minutes and 45–60 seconds, respectively. At 48 hours post-exposure, total RNA was isolated from excised skin samples and hybridized to Affymetrix GeneChip Porcine Genome Arrays (containing 20,201 genes). Both HD and thermal exposure promoted significant transcriptional changes where ...
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