John S. Graham
United States Department of the Army
SurgeryPathologyGene expressionMolecular biologyChemistryMicroarrayIn vitroIn vivoHuman skinWeanlingErythemaHairlessWound healingSulfur mustardLesionChromatographyHistopathologyMedicineMicroarray analysis techniquesBiologyTransepidermal water lossPharmacology
52Publications
18H-index
993Citations
Publications 52
Newest
#1Christopher H. Dalton (University of Birmingham)H-Index: 10
#2Charlotte A. Hall (HPA: Health Protection Agency)H-Index: 6
Last. Robert P. Chilcott (University of Hertfordshire)H-Index: 23
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AbstractPurpose: The aim of this study was to evaluate a candidate haemostat (WoundStat™), down-selected from previous in vitro studies, for efficacy as a potential skin decontaminant against the chemical warfare agent pinacoyl methylfluorophosphonate (Soman, GD) using an in vivo pig model.Materials and methods: An area of approximately 3 cm2 was dermatomed from the dorsal ear skin to a nominal depth of 100 µm. A discrete droplet of 14C-GD (300 µg kg−1) was applied directly onto the surface of t...
2 CitationsSource
#1Helen L. Lydon (University of Birmingham)H-Index: 4
#2Charlotte A. Hall (University of Birmingham)H-Index: 6
Last. Robert P. Chilcott (University of Hertfordshire)H-Index: 23
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This study used a damaged skin, porcine model to evaluate the in vivo efficacy of WoundStat™ for the decontamination of superficial, nerve agent-contaminated wounds. Anaesthetized animals were randomly assigned to either control (n = 7), no decontamination (n = 12) or WoundStat™ (n = 12) treatment groups. Pigs were exposed to a 5× LD50 dose of neat, radiolabelled S-[2-(diisopropylamino)ethyl]-O-ethyl methyl-phosphonothioate (VX; or equivalent volume of sterile saline for the control group) via a...
4 CitationsSource
#1Helen L. Lydon (University of Birmingham)H-Index: 4
#2Charlotte A. Hall (University of Birmingham)H-Index: 6
Last. Robert P. Chilcott (University of Hertfordshire)H-Index: 23
view all 6 authors...
6 CitationsSource
#1Charlotte A. Hall (University of Birmingham)H-Index: 6
#2Helen L. Lydon (University of Birmingham)H-Index: 4
Last. Robert P. Chilcott (University of Hertfordshire)H-Index: 23
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This is the peer reviewed version of the following article: Charlotte A. Hall, et al, 'The percutaneous toxicokinetics of Sulphur mustard in a damaged skin porcine model and the evaluation of WoundStat™ as a topical decontaminant', Journal of Applied Toxicology, July 2017, which has been published in final form at DOI: 10.1002/jat.3453. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. Copyright © 2017 John Wiley & Sons, Ltd.
4 CitationsSource
#1Jennifer L. Plahovinsak (Battelle Memorial Institute)H-Index: 3
#2Matthew Buccellato (Battelle Memorial Institute)H-Index: 3
Last. John S. Graham (United States Army Medical Research Institute of Chemical Defense)H-Index: 18
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AbstractThe inflammatory process plays an important role in sulfur mustard (HD) injury and HD pathogenesis, suggesting that anti-inflammatory treatments applied as soon as possible following HD injury may reduce tissue damage and accelerate healing. This study used the HD dermal weanling swine model to investigate the efficacy of two non-steroidal anti-inflammatory drugs, capsaicin and diclofenac, when applied in combination with the steroid, clobetasol. The therapeutic regimen was also investig...
2 CitationsSource
#2Charlotte A. Hall (University of Birmingham)H-Index: 6
#4James K. Chipman (University of Birmingham)H-Index: 46
Last. John Jenner (SU: Salisbury University)H-Index: 9
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This document is the Accepted Manuscript version of the following article: Lydon, H. L., Hall, C. A., Dalton, C. H., Chipman, J. K., Graham, J. S., and Chilcott, R. P., ‘Development of haemostatic decontaminants for treatment of wounds contaminated with chemical warfare agends. 3: Evaluation of in vitro topical decontamination efficacy using damaged skin’, Journal of Applied Toxicology, Vol. 37 (8): 976-984, February 2017. The Version of Record is available online at doi: doi: 10.1002/jat.3446. ...
10 CitationsSource
#1Charlotte A. Hall (University of Birmingham)H-Index: 6
#2Helen L. Lydon (University of Birmingham)H-Index: 4
Last. Robert P. Chilcott (University of Hertfordshire)H-Index: 23
view all 6 authors...
Charlotte A. Hall, et al, 'Development of haemostatic decontaminants for the treatment of wounds contaminated with chemical warfare agents. 1: Evaluation of in vitro clotting efficacy in teh presence of certain contaminants', vol. 35 (5): 536-542, May 2015, doi: 10.1002/jat.3019. Copyright © 2014 John Wiley & Sons, Ltd.
6 CitationsSource
#1Shlomit Dachir (Israel Institute for Biological Research)H-Index: 19
#2Maayan Cohen (Israel Institute for Biological Research)H-Index: 10
Last. Tamar Kadar (Israel Institute for Biological Research)H-Index: 26
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AbstractObjective: Macrophages are known to have key functions in almost every stage of wound healing and there is evidence for their beneficial effects in treating decubital ulcers and deep sternal wound infections in human. This study aimed to investigate the efficacy of a treatment with activated macrophages on ameliorating acute and long-term sulfur mustard (SM) induced skin injuries in the hairless guinea pig (HGP) model.Methods: HGP were exposed to SM vapor and treated with either a single...
8 CitationsSource
#1Thomas H. Snider (Battelle Memorial Institute)H-Index: 6
#2Mark R. Perry (Battelle Memorial Institute)H-Index: 4
Last. John S. GrahamH-Index: 18
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AbstractContext: Assessing the hazards of accidental exposure to toxic industrial chemical (TIC) vapors and evaluating therapeutic compounds or treatment regimens require the development of appropriate animal models.Objective: The objective of this project was to develop an exposure system for delivering controlled vapor concentrations of TICs to the skin of anesthetized weanling pigs. Injury levels targeted for study were superficial dermal (SD) and deep dermal (DD) skin lesions as defined hist...
3 CitationsSource
#1John S. Graham (DA: United States Department of the Army)H-Index: 18
#2Bruce A. Schoneboom (DA: United States Department of the Army)H-Index: 3
Abstract Sulfur mustard (2,2′-dichlorodiethyl sulfide; SM) is a potent vesicating chemical warfare agent that poses a continuing threat to both military and civilian populations. Significant SM injuries can take several months to heal, necessitate lengthy hospitalizations, and result in long-term complications affecting the skin, eyes, and lungs. This report summarizes initial and ongoing (chronic) clinical findings from SM casualties from the Iran–Iraq War (1980–1988), with an emphasis on cutan...
60 CitationsSource