An assessment of transcriptional changes in porcine skin exposed to bromine vapor.

Published on Jul 1, 2011in Journal of Biochemical and Molecular Toxicology3.606
· DOI :10.1002/JBT.20383
James V. Rogers16
Estimated H-index: 16
(Battelle Memorial Institute),
Jennifer A. Price8
Estimated H-index: 8
(Battelle Memorial Institute)
+ 4 AuthorsJohn S. Graham18
Estimated H-index: 18
Sources
Abstract
Bromine is an industrial chemical that can cause severe cutaneous burns. This study was a preliminary investigation into the effect of cutaneous exposure to bromine vapor using a weanling swine burn model and microarray analysis. Ventral abdominal sites were exposed to a mean calculated bromine vapor concentration of 0.69 g L−1 for 10 or 20 min. At 48 h postexposure, total RNA from skin samples was isolated, processed, and hybridized to Affymetrix GeneChip Porcine Genome Arrays. Expression analysis revealed that bromine vapor exposure for 10 or 20 min promoted similar transcriptional changes in the number of significantly modulated probe sets. A minimum of 83% of the probe sets was similar for both exposure times. Ingenuity pathways analysis revealed eight common biological functions among the top 10 functions of each experimental group, in which 30 genes were commonly shared among 19 significantly altered signaling pathways. Transcripts encoding heme oxygenase 1, interleukin-1β, interleukin 2 receptor gamma chain, and plasminogen activator inhibitor-1 were identified as common potential therapeutic targets for Phase II/III clinical trial or FDA-approved drugs. The present study is an initial assessment of the transcriptional responses to cutaneous bromine vapor exposure identifying molecular networks and genes that could serve as targets for developing therapeutics for bromine-induced skin injury. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:252–262, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20383
📖 Papers frequently viewed together
10 Citations
14 Citations
39 Citations
References60
Newest
#1Ian P. Dick (ICI: Imperial Chemical Industries)H-Index: 2
#2Robert C. Scott (ICI: Imperial Chemical Industries)H-Index: 11
Abstract— Pig skin has been shown to have similar histological and physiological properties to human skin and has been suggested as a good model for human skin permeability. In this series of experiments, the in-vitro permeability of pig ear skin was compared with human (abdominal) skin and rat (dorsal) skin using both hydrophilic (water, mannitol, paraquat) and lipophilic (aldrin, carbaryl, fluazifop-butyl) penetrants. Pig skin was found to have a closer permeability character than rat skin to ...
284 CitationsSource
#1Charles A. Dinarello (University of Colorado Denver)H-Index: 213
More than any other cytokine family, the interleukin (IL)-1 family is closely linked to the innate immune response. This linkage became evident upon the discovery that the cytoplasmic domain of the IL-1 receptor type I is highly homologous to the cytoplasmic domains of all Toll-like receptors (TLRs). Thus, fundamental inflammatory responses such as the induction of cyclooxygenase type 2, increased expression of adhesion molecules, or synthesis of nitric oxide are indistinguishable responses of b...
2,406 CitationsSource
#1Donald R. Gerecke (RU: Rutgers University)H-Index: 18
#2Minjun Chen (RU: Rutgers University)H-Index: 30
Last. Panos G. Georgopoulos (RU: Rutgers University)H-Index: 29
view all 8 authors...
Sulfur mustard (HD, SM), is a chemical warfare agent that within hours causes extensive blistering at the dermal–epidermal junction of skin. To better understand the progression of SM-induced blistering, gene expression profiling for mouse skin was performed after a single high dose of SM exposure. Punch biopsies of mouse ears were collected at both early and late time periods following SM exposure (previous studies only considered early time periods). The biopsies were examined for pathological...
39 CitationsSource
#1Leigh D Church (University of Birmingham)H-Index: 17
#2Michael F. McDermott (University of Leeds)H-Index: 65
Rilonacept in cryopyrin-associated periodic syndromes: the beginning of longer-acting interleukin-1 antagonism
20 CitationsSource
#4Marc G. Jeschke (UTMB: University of Texas Medical Branch)
Abstract The persistent inflammatory response induced by a severe burn increases patient susceptibility to infections and sepsis, potentially leading to multi-organ failure and death. In order to use murine models to develop interventions that modulate the post-burn inflammatory response, the response in mice and the similarities to the human response must first be determined. Here, we present the temporal serum cytokine expression profiles in burned mice in comparison to sham mice and human bur...
96 CitationsSource
#1James V. Rogers (Battelle Memorial Institute)H-Index: 16
#2James N. McDougal (Wright State University)H-Index: 13
Last. John S. GrahamH-Index: 18
view all 5 authors...
In military and civilian environments, serious cutaneous damage can result from thermal burns or exposure to the blistering agent sulfur mustard [bis (2-chloroethyl) sulfide; HD]. Similar therapies have historically been used to treat cutaneous thermal and HD injuries; however, the underlying molecular mechanisms of tissue damage and wound healing may differ between the types of burns. Using microarray analysis, this study assessed the transcriptional responses to cutaneous HD and thermal injury...
14 CitationsSource
#1Jennifer A. Price (Battelle Memorial Institute)H-Index: 8
#2James V. Rogers (Battelle Memorial Institute)H-Index: 16
Last. John S. GrahamH-Index: 18
view all 7 authors...
Abstract Bromine is an industrial chemical that is irritating to the skin and causes cutaneous burns. An important factor in selecting or developing an effective treatment is to understand the underlying molecular mechanisms of tissue damage and wound healing. This study used a weanling swine burn model and microarray analysis to evaluate the effect of exposure length and sampling times on the transcriptional changes in response to cutaneous bromine injury. Ventral abdominal sites ( N = 4/treatm...
10 CitationsSource
#1Xinliang Mao (OICR: Ontario Institute for Cancer Research)H-Index: 29
#2Aaron D. Schimmer (OICR: Ontario Institute for Cancer Research)H-Index: 73
Abstract 5-Chloro-7-iodo-quinolin-8-ol (Clioquinol) is a halogenated 8-hydroxyquinoline that was used in 1950–1970s as an oral anti-parasitic agent for the treatment and prevention of intestinal amebiasis. However in the 1970s oral Clioquinol was withdrawn from the market due to reports of neurotoxicity in Japanese patients. Recently, reports have demonstrated that Clioquinol has activities beyond its use as an antimicrobial. For example, Clioquinol inhibits the function of the proteasome and di...
87 CitationsSource
#2Sharukh D. ShroffH-Index: 1
Last. Daniel L. KastnerH-Index: 102
view all 13 authors...
Objective Familial cold autoinflammatory syndrome (FCAS) is caused by mutations in the CIAS1 gene, leading to excessive secretion of interleukin-1β (IL-1β), which is associated with cold-induced fevers, joint pain, and systemic inflammation. This pilot study was conducted to assess the safety and efficacy of rilonacept (IL-1 Trap), a long-acting IL-1 receptor fusion protein, in patients with FCAS. Methods Five patients with FCAS were studied in an open-label trial. All patients received an initi...
186 CitationsSource
#1Kiran PatilH-Index: 11
#2Lars BellnerH-Index: 20
Last. Michal L. Schwartzman (NYMC: New York Medical College)H-Index: 62
view all 6 authors...
PURPOSE. Heme oxygenase (HO) is considered a fundamental endogenous immunomodulatory, cytoprotective, and anti-inflammatory system. This protective function is primarily ascribed to the inducible HO-1. The authors examined the effect of HO-1 induction on corneal inflammation and wound healing in mice undergoing epithelial injury. METHODS. C57BL6 mice were treated with SnCl2 the day before epithelial injury and once daily thereafter. The corneal epithelium was removed with the use of a corneal ru...
66 CitationsSource
Cited By13
Newest
Source
#1Anderson R. Aguillón (UFRJ: Federal University of Rio de Janeiro)H-Index: 2
#2Raquel A. C. Leão (UFRJ: Federal University of Rio de Janeiro)H-Index: 10
Last. Rodrigo O. M. A. de Souza (UFRJ: Federal University of Rio de Janeiro)H-Index: 23
view all 4 authors...
The current state of evidence and recommendations for cannabidiol (CBD) and its health effects change the legal landscape and aim to destigmatize its phytotherapeutic research. Recently, some countries have included CBD as an antiepileptic product for compassionate use in children with refractory epilepsy. The growing demand for CBD has led to the need for high-purity cannabinoids on the emerging market. The discovery and development of approaches toward CBD synthesis have arisen from the succes...
Source
#1Anjaly MathewH-Index: 1
#2Beena Mathew (Mahatma Gandhi University)H-Index: 20
Last. Ebey P. Koshy (Saint Joseph's College)H-Index: 7
view all 3 authors...
A novel, microwave safe and recyclable polymeric reagent was prepared by anchoring bromoderivative of 2-pyrrolidone in the polymer matrix of 3% tetraethyleneglycol diacrylate cross-linked polystyrene. The synthetic utility of the prepared polymeric reagent was demonstrated by the efficient bromination of unsaturated compounds, activated aromatic compounds and also for the α-bromination of ketones under microwave activation. Operational simplicity, moderate to excellent yield of the products, sho...
Source
#1Ahmed Lazrak (UAB: University of Alabama at Birmingham)H-Index: 26
#2Weifeng Song (UAB: University of Alabama at Birmingham)H-Index: 13
Last. Sadis Matalon (UAB: University of Alabama at Birmingham)H-Index: 76
view all 7 authors...
Chlorine (Cl2 ) and bromine (Br2 ) are produced in large quantities throughout the world and used in the industry and the sanitation of water. These halogens can pose a significant threat to public health when released into the atmosphere during transportation and industrial accidents, or as acts of terrorism. In this review, we discuss the evidence showing that the activity of Cl2 and Br2 , and of products formed by their interaction with biomolecules, fragment high-molecular-weight hyaluronan ...
2 CitationsSource
#1Anjaly MathewH-Index: 1
#2Beena MathewH-Index: 20
Last. Ebey P. KoshyH-Index: 7
view all 3 authors...
We present herein the synthesis and structural characterization of a novel, recyclable and microwave stable polymeric reagent prepared by anchoring bromoderivative of oxazolidone into the 3D matrix of divinylbenzene crosslinked polystyrene and its application for the bromination of alkenes, activated aromatic compounds and for the α-halogenation of ketones under microwave irradiation. The advantages of this protocol include a simple workup procedure, high product yield, shorter reaction time as ...
Source
#1Anjaly Mathew (Saint Joseph's College)
#2Beena Mathew (Mahatma Gandhi University)H-Index: 20
Last. Ebey P. Koshy (Saint Joseph's College)H-Index: 7
view all 3 authors...
Here a novel polymeric reagent was synthesised by anchoring bromoderivatives of pyrrolidone into the 3D matrix of divinylbenzene cross linked polystyrene and successfully employed for the decarboxylative bromination of trans-cinnamic acid to trans-β-bromostyrene under microwave exposure. 100% conversion and selectivity in a concise period of time under solvent-free condition and simple workup procedure are the foremost attraction of this method. The prepared polymeric reagent exhibited good cycl...
Source
#1Shreen Deeb Nusair (JUST: Jordan University of Science and Technology)H-Index: 4
#2Mohammad Jamil Almasaleekh (JUST: Jordan University of Science and Technology)H-Index: 1
Last. Mohammad J. Alkhatatbeh (JUST: Jordan University of Science and Technology)H-Index: 9
view all 4 authors...
Abstract Bromide (Br − ) is a bromine atom with a negative charge which is released mainly in the production of pesticides and flame retardants. It is also found naturally in seawater. Br¯ has been associated with many detrimental effects such as respiratory problems, gastric hemorrhages, and dermal burns. The aim of the study was to monitor serum bromide in humans and to correlate its level with genotoxicity and apoptosis in human. The study utilized comet assay, to measure DNA damage in periph...
3 CitationsSource
#1James A. LambertH-Index: 3
Last. Sadis MatalonH-Index: 76
view all 13 authors...
Inhalation of oxidant gases has been implicated in adverse outcomes in pregnancy, but animal models to address mechanisms and studies to identify potential pregnancy-specific therapies are lacking. Herein, we show that inhalation of bromine at 600 parts per million for 30 minutes by pregnant mice on the 15th day of embryonic development results in significantly lower survival after 96 hours than an identical level of exposure in nonpregnant mice. On the 19th embryonic day, bromine-exposed pregna...
19 CitationsSource
#1Adam Lam (UAB: University of Alabama at Birmingham)H-Index: 7
#2Nilam Vetal (UAB: University of Alabama at Birmingham)H-Index: 3
Last. Saurabh Aggarwal (UAB: University of Alabama at Birmingham)H-Index: 21
view all 4 authors...
Bromine (Br2 ) gas inhalation poses an environmental and occupational hazard resulting in high morbidity and mortality. In this review, we underline the acute lung pathology (within 24 h of exposure) and potential therapeutic interventions that may be utilized to mitigate Br2 -induced human toxicity. We discuss our latest published data, which suggest that an increase in heme-dependent tissue injury underlies the pathogenesis of Br2 toxicity. Our study was based on previous findings that demonst...
19 CitationsSource
#2Arun Jyoti BorahH-Index: 8
Last. Prodeep PhukanH-Index: 23
view all 3 authors...
Bromination is one of the most important transformations in organic synthesis and can be carried out using bromine and many other bromo compounds. Use of molecular bromine in organic synthesis is well-known. However, due to the hazardous nature of bromine, enormous growth has been witnessed in the past several decades for the development of solid bromine carriers. This review outlines the use of bromine and different bromo-organic compounds in organic synthesis. The applications of bromine, a to...
197 CitationsSource