Hepatoprotective Effect of Active Constituents Isolated from Mung Beans (Phaseolus radiatus L.) in an Alcohol‐Induced Liver Injury Mouse Model

Published on Oct 1, 2014in Journal of Food Biochemistry1.662
· DOI :10.1111/JFBC.12083
Ting Liu6
Estimated H-index: 6
(SPU: Shenyang Pharmaceutical University),
Xiao Han Yu1
Estimated H-index: 1
(SPU: Shenyang Pharmaceutical University)
+ 6 AuthorsZhi Guo Yu (SPU: Shenyang Pharmaceutical University)
The aim of the present study was to investigate the potential hepatoprotective effects of flavonoids from mung beans in an alcohol-induced liver injury mouse model. Mung bean extract (ME) was obtained by extraction with 75% ethanol, followed by concentration and lyophilization. Two major flavonoids (TMF) were isolated from mung beans and authenticated as vitexin and isovitexin. The hepatoprotective effect of ME and TMF against liver damage was investigated by measuring the serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) as well as the hepatic level of malondialdehyde (MDA) and superoxide dismutase (SOD). The results obtained demonstrated that alcohol-induced hepatic pathological changes, elevations in ALT, AST and MDA, and a decrease in SOD were significantly inhibited in animals given ME and TMF. In conclusion, mung beans had a significant protective effect against alcohol-induced liver injury and its hepatoprotective effect was mainly produced by TMF. Practical Applications Numerous studies have shown that the consumption of mung beans promotes health. This is because it contains several important bioactive compounds such as protein, carbohydrates, minerals, flavonoids and vitamins that have been isolated from mung beans in earlier investigations. Mung beans are effective against heatstroke, fever, diabetes and diseases related to the liver. It is also effective for detoxication of minerals, toxic herbs and pesticides. The findings of this study demonstrate the hepatoprotective effect of mung bean extract and its active ingredients. It suggests that mung bean could be used as a nutraceutical and functional food for the treatment of alcoholic liver disease.
📖 Papers frequently viewed together
96 Citations
37 Citations
#1He Li (CAU: China Agricultural University)H-Index: 3
#2Dongdong Cao (CAU: China Agricultural University)H-Index: 5
Last. Weibo Jiang (CAU: China Agricultural University)H-Index: 28
view all 5 authors...
Abstract Mung bean soup (MBS) has been traditionally taken as a kind of health food in China. To learn the mechanisms underlying its health benefits, antioxidant capacities of the soup prepared with three cultivars of mung bean were measured. The highest DPPH radical scavenging or ferric reducing activity was observed in soup of mung bean cv. Huang . The MBS of cv. Huang and Mao exhibited higher ABTS + reducing activities than MBS of cv. Ming . The two major flavonoids in the MBS were purified a...
45 CitationsSource
#1Mingchun Wang (NAU: Nanjing Agricultural University)H-Index: 15
#2Peilei ZhuH-Index: 1
Last. Xiaoxiong Zeng (NAU: Nanjing Agricultural University)H-Index: 62
view all 6 authors...
Abstract The fresh fleshy peduncles of Hovenia dulcis have been used as a food supplement and traditional herbal medicine for the treatment of liver diseases and alcoholic poisoning for more than a millennium. The objectives of the present study, therefore, were to determine the antioxidant activity of polysaccharides from the peduncles of H. dulcis (HDPS) and to evaluate its hepatoprotective effect on acute alcohol-induced liver injury in mice. HDPS, prepared by hot water extraction, ethanol pr...
109 CitationsSource
#1Dongdong Cao (CAU: China Agricultural University)H-Index: 5
#2He Li (CAU: China Agricultural University)H-Index: 3
Last. Weibo Jiang (CAU: China Agricultural University)H-Index: 28
view all 9 authors...
Background It is a widespread belief in Asian countries that mung bean soup (MBS) may afford a protective effect against heat stress. Lack of evidence supports MBS conferring a benefit in addition to water. Results Here we show that vitexin and isovitexin are the major antioxidant components in mungbean (more than 96% of them existing in the bean seed coat), and both of them could be absorbed via gavage into rat plasma. In the plasma of rats fed with mungbean coat extract before or after exposur...
70 CitationsSource
#1Viviana R. Lucero López (National University of San Luis)H-Index: 3
#2Gabriela Silvina Razzeto (National University of San Luis)H-Index: 3
Last. Nora Lilian Escudero (National University of San Luis)H-Index: 9
view all 4 authors...
Amaranth constitutes a valuable pseudocereal, due to its nutritional quality and its nutraceutical properties, which contribute to improve human health. This work evaluated the effect of a diet based on Amaranthus hypochondriacus (Ah) seed on oxidative stress and antioxidant status in the liver of rats sub-chronically exposed to ethanol. The seed extract was investigated for antioxidant capacity in vitro, showing an adequate content of total phenols and antioxidant activity elevated. For in vivo...
28 CitationsSource
Alcohol damages every organ and system in the body. The most important effects from a clinical point of view relate to diseases of the circulatory, nervous and hepato-gastrointestinal systems. In the digestive tract the effects range from increased intestinal transit time and gastrophaties, leading to classical early morning nausea and diarrhea, through to significant malabsorption and chronic pancreatitis. In this review the mechanisms of alcoholic damage have been evaluated with particular ref...
47 Citations
#1Farooq Anwar (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 72
#2Sajid Latif (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 18
Last. Muhammad Ashraf (H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 114
view all 5 authors...
: Seeds of 4 cultivars (M-1, M-6, NM-92, and NM-98) of mungbean [Vigna radiata (L.) Wilczek] were investigated for proximate composition, antioxidant potential, fatty acids, tocopherols, and minerals profiles. Hexane-extracted seed oil content of the investigated cultivars of mungbeans ranged from 1.20% to 1.56%. Mungbean seeds were found to be a rich source of protein (20.97% to 31.32%). The contents of Fe, Cu, Mg, Na, K, Ca, and Zn were found to be 105.8 to 190.9, 4.8 to 6.3, 48.6 to 51.7, 382...
87 CitationsSource
#1Reena Randhir (UMass: University of Massachusetts Amherst)H-Index: 15
#2Kalidas Shetty (UMass: University of Massachusetts Amherst)H-Index: 74
Abstract Mung bean substrate was enriched with phenolic antioxidants and levo-dihydroxy phenylalanine ( l -DOPA) through solid-state bioconversion (SSB) by Rhizopus oligosporus , with the goal to enhance health-linked functionality. The alpha-amylase inhibition linked to diabetes management and Helicobacter pylori inhibition linked to peptic ulcer management were investigated in bioprocessed extracts. The protein content and β-glucosidase activity of the substrate which are indicators of effecti...
109 CitationsSource
#1Christopher P. Day (Newcastle University)H-Index: 110
Abstract: While the vast majority of heavy drinkers and individuals with obesity, insulin resistance, and the metabolic syndrome will have steatosis, only a minority will ever develop steatohepatitis, fibrosis, and cirrhosis. Genetic and environmental risk factors for advanced alcoholic liver disease (ALD) and non-alcoholic fatty liver disease (NAFLD) seem likely to include factors that influence the severity of steatosis and oxidative stress, the cytokine milieu, the magnitude of the immune res...
138 CitationsSource
#1Annagiulia Gramenzi (UNIBO: University of Bologna)H-Index: 31
#2Fabio Caputo (UNIBO: University of Bologna)H-Index: 28
Last. Mauro Bernardi (UNIBO: University of Bologna)H-Index: 89
view all 7 authors...
Summary Background Alcoholic liver disease has a known aetiology but a complex and incompletely known pathogenesis. It is an extremely common disease with significant morbidity and mortality, but the reason why only a relatively small proportion of heavy drinkers progress to advanced disease remains elusive. Aim To recognize the factors responsible for the development and progression of alcoholic liver disease, in the light of current knowledge on this matter. Methods We performed a structured l...
156 CitationsSource
#1Guan-Hong Li (JXAU: Jiangxi Agricultural University)H-Index: 2
#2Ju-Zhen Wan (JXAU: Jiangxi Agricultural University)H-Index: 1
Last. Yonghui Shi (Yangtze University)H-Index: 31
view all 4 authors...
Mung bean protein isolates were hydrolyzed for 2 h by Alcalase. The generated hydrolysate showed angiotensin I-converting enzyme (ACE) inhibitory activity with the IC50 value of 0.64 mg protein/ml. Three kinds of novel ACE inhibitory peptides were isolated from the hydrolysate by Sephadex G-15 and reverse-phase high performance liquid chromatography (RP-HPLC). These peptides were identified by amino acid composition analysis and matrix assisted-laser desorption/ionization time-of-flight tandem m...
93 CitationsSource
Cited By11
#1Liang ZhaoH-Index: 16
#2Arshad MehmoodH-Index: 9
Last. Chengtao WangH-Index: 20
view all 7 authors...
Alcoholic liver disease (ALD) is one type of liver disease, causing a global healthcare problem and mortality. The liver undergoes tissue damage by chronic alcohol consumption because it is the main site for metabolism of ethanol. Chronic alcohol exposure progresses from alcoholic fatty liver (AFL) to alcoholic steatohepatitis (ASH), which further lead to fibrosis, cirrhosis, and even hepatocellular cancer. Therapeutic interventions to combat ALD are very limited such as use of corticosteroids. ...
1 CitationsSource
#1Dianzhi Hou (CAU: China Agricultural University)H-Index: 5
#2Qingyu Zhao (CAU: China Agricultural University)H-Index: 4
Last. Qun Shen (CAU: China Agricultural University)H-Index: 16
view all 5 authors...
Purpose Obesity, a strong risk factor for metabolic disorder, has become a major impediment for public health globally. The objective of this study was to assess the anti-obesity effect of mung bean, and the relationship between the gut microbiota modulatory effects of mung bean and the prevention of obesity.
12 CitationsSource
#1Dianzhi Hou (CAU: China Agricultural University)H-Index: 5
#2Qingyu Zhao (CAU: China Agricultural University)H-Index: 4
Last. Qun Shen (CAU: China Agricultural University)H-Index: 16
view all 6 authors...
Abstract Mung bean is shown having several health benefits, but a little bit of knowledge is known about its effects on high-fat diet (HFD)-induced obesity or its relationship with gut microbiota composition changes. Here, it was observed that consumption of HFD supplemented with cooked mung bean (30%, w/w) for 12 weeks effectively alleviated body weight gain and lipid metabolic disorders, which was accompanied by a decrease in hepatic steatosis and adipocyte size. Furthermore, high-throughput s...
22 CitationsSource
#1Dianzhi Hou (CAU: China Agricultural University)H-Index: 5
#2Laraib Yousaf (CAU: China Agricultural University)H-Index: 5
Last. Qun Shen (CAU: China Agricultural University)H-Index: 16
view all 8 authors...
Mung bean (Vigna radiata L.) is an important pulse consumed all over the world, especially in Asian countries, and has a long history of usage as traditional medicine. It has been known to be an excellent source of protein, dietary fiber, minerals, vitamins, and significant amounts of bioactive compounds, including polyphenols, polysaccharides, and peptides, therefore, becoming a popular functional food in promoting good health. The mung bean has been documented to ameliorate hyperglycemia, hype...
68 CitationsSource
#1Kumar Ganesan (Hong Kong Baptist University)H-Index: 15
#2Baojun Xu (Hong Kong Baptist University)H-Index: 54
Abstract The seeds and sprouts of mung bean are very common cruise in Asia. Evidence showed that bioactive compounds in mung bean have emerged as an increasing scientific interest due to their role in the prevention of degenerative diseases. All data of in vitro, in vivo and clinical studies of mung bean and its impact on human health were collected from a library database and electronic search. Botanical, phytochemical and pharmacological information was gathered and orchestrated. Remarkable st...
62 CitationsSource
#1Yan Bai (SPU: Shenyang Pharmaceutical University)H-Index: 4
#2Qili Zhang (SPU: Shenyang Pharmaceutical University)H-Index: 7
Last. Zhiguo Yu (SPU: Shenyang Pharmaceutical University)H-Index: 16
view all 8 authors...
The main polyphenols in mung bean (Vigna radiata L.) seed (MBS), an edible legume with various biological activities, are C-glycosyl flavones (vitexin, isovitexin, isovitexin-6″-O-α-l-glucoside, and dulcinoside). In our study, a validated ultrahigh-performance liquid chromatography–tandem mass spectrometry (UHPLC–MS/MS) method was developed to quantitate the concentrations of four C-glycosyl flavones from MBS extracts in the plasma and various tissues of rats and successfully applied to study th...
10 CitationsSource
Folium Microcos (FM), the leaves of Microcos paniculata L., shows various biological functions including antioxidant activity and α-glucosidase inhibitory effect. However, its therapeutic potential in acute liver injury is still unknown. This study investigated the hepatoprotective effect and underlying mechanisms of the polyphenol-enriched fraction (FMF) from Folium Microcos. FMF exhibited strong free radical scavenging activities and prevented HepG2/Hepa1–6 cells from hydrogen peroxide- (H2O2-...
35 CitationsSource
#1Jiaoyan Ren (SCUT: South China University of Technology)H-Index: 35
#2Yamei Zheng (SCUT: South China University of Technology)H-Index: 2
Last. Wenzhen Liao (U of S: University of Saskatchewan)H-Index: 19
view all 5 authors...
The flavonoids (POFs) from the leaves of Platycladus orientalis (L.) Franco were purified using six different macroporous adsorption resins including polar resins NKA-9 and ADS-F8, semi-polar resins ADS-17 and AB-8, and non-polar resins D101 and ADS-5. Among semi-polar resins, AB-8 demonstrated the best adsorption and desorption capacities with an adsorption ratio of 86% and a desorption ratio of 52%. According to the Simultaneous Thermogravimetry-Differential Scanning Calorimetry (STA/TG-DSC) a...
32 CitationsSource
#1Yan Bai (SPU: Shenyang Pharmaceutical University)H-Index: 1
#2Jiawei Chang (SPU: Shenyang Pharmaceutical University)H-Index: 2
Last. Zhiguo Yu (SPU: Shenyang Pharmaceutical University)H-Index: 1
view all 7 authors...
Mung bean (Vigna radiata L.) seeds (MBS) contain abundant nutrients with biological activities. This study was aimed to isolate key bioactive components from MBS with antioxidant and myocardial preservation activities. A new flavonoid C-glycoside, isovitexin-6″-O-α-l-glucoside, and 14 known compounds were obtained. Their structures were identified by extensive 1D and 2D NMR and FT–ICR–MS spectroscopic analyses. The antioxidant activities of these compounds were evaluated. Compounds 1–5 and 7–10 ...
25 CitationsSource