Icariin, a flavonoid from the herb Epimedium enhances the osteogenic differentiation of rat primary bone marrow stromal cells.

Published on Dec 1, 2005in Die Pharmazie1.198
Chen Km3
Estimated H-index: 3
Ge Bf3
Estimated H-index: 3
+ 3 AuthorsWang Y1
Estimated H-index: 1
The herb Epimedium has long been used in Traditional Chinese Medicine to treat bone fracture and prevent osteoporosis. Researchers believe that the flavonoids contained in the herb are the effective component for this activity. However, no single flavonoid has been studied for its effect on bone-related cells. In the present study, icariin, one of the major flavonoids of the herb, supplemented the primary culture medium of rat bone marrow stromal cells (rMSCs) at 0.1 μM, 1 μM and 10 μM respectively. It was found that icariin stimulated the proliferation of rMSCs and increased the number of CFU-F stained positive for alkaline phosphatase in a dose-dependent manner. Icariin also dose-dependently increased the alkaline phosphatase activity, osteoalcin secretion and calcium deposition level of rMSCs during osteogenic induction. The addition of 10 μM icariin caused four times more mineralized bone nodules to be formed by rMSCs than in the control. The results demonstrated that icariin should be an effective component for bone-strengthening activity, and one of the mechanisms is to stimulate the proliferation and enhance the osteogenic differentiation of MSCs.
📖 Papers frequently viewed together
247 Citations
4 Authors (Hua Nian, ..., L.-L. Xu)
204 Citations
5 Authors (Tsai Pei Hsieh, ..., Man Hai Liu)
159 Citations
Cited By106
#1Dageng Huang (Xi'an Jiaotong University)H-Index: 8
#2Yangyang Wang (NPU: Northwestern Polytechnical University)H-Index: 1
Last. Dingjun Hao (Xi'an Jiaotong University)H-Index: 18
view all 9 authors...
Postmenopausal osteoporosis (PMOP) is a major global public health concern and older women are more susceptible to experiencing fragility fractures. Our study investigated the associations between circulating proteins with bone mineral density (BMD) in postmenopausal women with or without low BMD (osteoporosis and osteopenia) using a tandem mass tag (TMT) labeling proteomic experiment and parallel reaction monitoring testing. Across all plasma samples, we quantitatively measured 1,092 proteins, ...
#1Kyeongseok Kim (Konkuk University)H-Index: 11
#2Ahmed Abdal Dayem (Konkuk University)H-Index: 17
Last. Ssang-Goo Cho (Konkuk University)H-Index: 37
view all 11 authors...
Efficient maintenance of the undifferentiated status of human pluripotent stem cells (hiPSCs) is crucial for producing cells with improved proliferation, survival and differentiation, which can be successfully used for stem cell research and therapy. Here, we generated iPSCs from healthy donor peripheral blood mononuclear cells (PBMCs) and analyzed the proliferation and differentiation capacities of the generated iPSCs using single cell NGS-based 24-chromosome aneuploidy screening and RNA sequen...
4 CitationsSource
#1Bin Zhao (SDU: Shandong University)H-Index: 4
#2Yixuan Xiong (SDU: Shandong University)H-Index: 3
Last. Xin Xu (SDU: Shandong University)H-Index: 9
view all 6 authors...
Rutin is one of the flavonoids found in fruits and vegetables. Recent reports have revealed that rutin is a major player in proliferation and bone development. However, data on how rutin regulates ...
4 CitationsSource
Icariin (ICA) is obtained from Epimedium brevicornu maxim and exploited to remedy miscellaneous cancers. But the role of ICA in medulloblastoma remains hazy. The research delved into the antitumor activity of ICA in medulloblastoma DAOY cells. ICA with diverse concentrations was utilized to stimulate DAOY cells, and the biological functions of ICA in medulloblastoma DAOY cells were examined. Then, the relative SPARC expression was determined in ICA-managed DAOY cells, and the pc-SPARC vector was...
2 CitationsSource
#1Yuexin Xu (Nanjing Medical University)H-Index: 3
#2Lingyun Li (Nanjing Medical University)H-Index: 3
Last. Changyan Ma (Nanjing Medical University)H-Index: 8
view all 6 authors...
Abstract Osteoporosis is a bone disease characterized by microarchitectural deterioration, low bone mass, and increased risk of fractures. Icariin (ICA), an active flavonoid glucoside isolated from Herba epimedii (HEF), is a potent stimulator of osteogenic differentiation and has potential applications for preventing bone loss in postmenopausal women. However, the molecular mechanism underlying the osteogenic effect of ICA has not yet been fully elucidated. In this study, we report that ICA trea...
4 CitationsSource
#1Sara Zadegan (UT: University of Tehran)H-Index: 1
#2Jhamak Nourmohammadi (UT: University of Tehran)H-Index: 13
Last. Nooshin Haghighipour (Pasteur Institute of Iran)H-Index: 14
view all 4 authors...
Abstract The purpose of this study was to investigate physical, mechanical, and osteogenic properties of silk fibroin (SF) nanofibers containing Urtica dioica L. (nettle) extract at different concentrations. In this respect, the successful incorporation of nettle in SF nanofibers was analyzed and then confirmed through Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC). The mean fiber diameter, water uptake, breaking strain, cellular attachment, and prolif...
8 CitationsSource
#1Shuang Gu (ZJSU: Zhejiang Gongshang University)H-Index: 1
#2Rong Zhou (ZJSU: Zhejiang Gongshang University)H-Index: 1
Last. Xiangyang Wang (ZJSU: Zhejiang Gongshang University)H-Index: 1
view all 3 authors...
: To compare the effect of Epimedium brevicornum Maxim., Cynomorium songaricum Rupr. and Morindae officinalis F.C. How on enhancing sexual function effect, mice were administered gavage by the extracts from three materials, serum testosterone and nitric oxide(NO) levels in male mice were detected and the changes of sexual organs were measured. Results showed that testosterone level, NO level and sexual organs weights of Epimedium brevicornum Maxim. treatment were significantly (p < 0.01) higher ...
2 CitationsSource
#1Min Gong (Huada: Beijing University of Chemical Technology)H-Index: 9
#2Chi Cheng (Huada: Beijing University of Chemical Technology)H-Index: 3
Last. Liqun Zhang (Huada: Beijing University of Chemical Technology)H-Index: 72
view all 8 authors...
Abstract Due to the significant role of the periosteum in bone regeneration, we hypothesised that using a specially engineered artificial periosteum could lead to an enhancement in osteogenesis in bone grafts. Herein, we describe our work aimed at fabricating an electrospun fibrous membrane as an artificial periosteum that exhibits flexibility, permeability and osteoinduction. This membrane was designed to cover the complex surface of bone grafts to facilitate and accelerate bone regeneration. T...
17 CitationsSource
#1Yuan Luo (Tongji University)H-Index: 1
#2Yiwen Zhang (Tongji University)H-Index: 1
Last. Yuanliang Huang (Tongji University)H-Index: 1
view all 3 authors...
: BACKGROUND The aim of this study was to determine the role of icariin, a Chinese traditional herbal medicine extracted from Epimedium, in osteoarthritis (OA), using the murine anterior cruciate ligament transection (ACLT)-induced model of OA and micromass culture of murine chondrocytes. MATERIAL AND METHODS Twenty-four three-month-old C57/6J mice were randomly divided into three groups: the sham group (no surgery and joint injection with normal saline) (N=8); the ACLT + ICA group (ACLT surgery...
6 CitationsSource