Colorimetric immunoassay via smartphone based on Mn2+-Mediated aggregation of AuNPs for convenient detection of fumonisin B1

Fumonisin B1 (FB1) is one of the most toxic and widely distributed subtypes of the 20 fumonisins, which has attracted much attention in food and feed safety. In this study, a convenient colorimetric immunoassay was proposed for the detection of FB1 based on Mn2+-mediated aggregation of gold nanoparticles (AuNPs). After hydrolyzing of ascorbic acid 2-phosphate (AAP) by alkaline phosphatase (ALP), the generated ascorbic acid (AA) decomposes brown...