An Enhanced Lateral Flow Assay Based on Aptamer-Magnetic Separation and Multifold AuNPs for Ultrasensitive Detection of Salmonella Typhimurium in Milk.

Published on Jul 11, 2021in Foods4.35
· DOI :10.3390/FOODS10071605
Pingping Gao1
Estimated H-index: 1
Lihan Wang2
Estimated H-index: 2
+ 7 AuthorsYujun Jiang8
Estimated H-index: 8
In this paper, a novel and ultrasensitive lateral flow assay (LFA) based on aptamer–magnetic separation, and multifold Au nanoparticles (AuNPs) was developed for visual detecting Salmonella enterica ser. Typhimurium (S. Typhimurium). The method realized magnetic enrichment and signal transduction via magnetic separation and achieved signal amplification through hybridizing AuNPs–capture probes and AuNPs–amplification probes to form multifold AuNPs. Two different thiolated single-strand DNA (ssDNA) on the AuNPs–capture probe played different roles. One was combined with the AuNPs–amplification probe on the conjugate pad to achieve enhanced signals. The other was connected to transduction ssDNA1 released by aptamer–magnetic capture of S. Typhimurium, and captured by the T-line, forming a positive signal. This method had an excellent linear relationship ranging from 8.6 × 102 CFU/mL to 8.6 × 107 CFU/mL with the limit of detection (LOD) as low as 8.6 × 100 CFU/mL in pure culture. In actual samples, the visual LOD was 4.1 × 102 CFU/mL, which did not carry out nucleic acid amplification and pre-enrichment, increasing three orders of magnitudes than unenhanced assays with single–dose AuNPs and no magnetic separation. Furthermore, the system showed high specificity, having no reaction with other nontarget strains. This visual signal amplificated system would be a potential platform for ultrasensitive monitoring S. Typhimurium in milk samples.
#1Ahmed Esmael (Banha University)H-Index: 6
#2Ehab Azab (Taif University)H-Index: 10
Last. A.M. Abdelatty (Cairo University)H-Index: 6
view all 8 authors...
Foodborne salmonellosis is a global threat to public health. In the current study, we describe the isolation and characterization of two broad-spectrum, lytic Salmonella phages: SPHG1 and SPHG3 infecting a multidrug-resistant Salmonella Typhimurium EG.SmT3. Electron microscopy and whole genome analysis identified SPHG1 as a Myovirus, while SPHG3 as a new member of the genus "Kuttervirus" within the family Ackermannviridae. SPHG1 and SPHG3 had a lysis time of 60 min. with burst sizes of 104 and 1...
#1Lijun Wang (Xihua University)H-Index: 3
#2Tianyu Liao (Xihua University)H-Index: 1
Last. Xianggui Chen (Xihua University)H-Index: 7
view all 7 authors...
Abstract Development of a rapid and sensitive method for Salmonella spp. detection is of great importance for ensuring food product safety due to its low infective dose. In this study, a colorimetric method based on the peroxidase-like activity of Cu(II)-modified reduced graphene oxide nanoparticles (Cu2+-rGO NPs) and PCR was successfully developed to detect Salmonella spp. in milk. Under optimal conditions, the developed colorimetric method exhibited high sensitivity and strong specificity for ...
#1Rui LiuH-Index: 2
#2Zhiying WangH-Index: 4
Last. Shuming Yang (CAAS: Civil Aviation Authority of Singapore)H-Index: 1
view all 5 authors...
ABSTRACT Caused by Salmonella pullorum (S. pullorum), pullorosis is a bacterial disease threatening the poultry industry and has been listed as the bacterial disease to be eliminated by the government. However, antibiotic treatment of pullorosis has become increasingly difficult, resulting in severe influences on the sustainable development of poultry. Abuse of antibiotics may cause global drug-resistant problems. Hence, early diagnosis of young chickens and accurate treatment of sick chickens a...
#1Zhen Huang (NCU: Nanchang University)H-Index: 11
#2Yihui Liu (CAS: Chinese Academy of Sciences)H-Index: 1
Last. Weihua Lai (NCU: Nanchang University)H-Index: 35
view all 7 authors...
Abstract Upconversion nanoparticles (UCNPs) have been used as probes in lateral flow immunoassays (LFIAs), because the probe provides an excellent signal-to-noise ratio by eliminating background-fluorescence from biological samples. However, the low photoluminescence efficiency, unfavorable hydrophilic modification and poor antibody conjugation are insurmountable challenges for the application of UCNPs to LFIAs. In this work, we have made a breakthrough by integrating friendly ligand-exchange an...
#1Sihan Chen (NEAU: Northeast Agricultural University)H-Index: 5
#2Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 7 authors...
Abstract A sensitive and accurate strategy was presented based on gold nanoparticles (AuNPs) for visual detecting viable Salmonella typhimurium (S. typhimurium). In this paper, dual aptamers were used to capture S. typhimurium and PCR amplification, respectively. The highly stable network structure formed by the hybridization between amplicons and AuNPs-probes protects AuNPs from self-aggregation and color change in the presence of salt. Under optimized conditions, the ratio of the absorbance in...
#1Yu Li (NCU: Nanchang University)H-Index: 7
#2Xirui Chen (NCU: Nanchang University)H-Index: 7
Last. Yonghua Xiong (NCU: Nanchang University)H-Index: 47
view all 7 authors...
Escherichia coli O157:H7 is a common harmful foodborne pathogen that can cause severe diseases at low infectious doses. Traditional lateral flow immunoassay (LFIA) for the rapid screening of E. coli O157:H7 in food suffers from low sensitivity due to its dependence on 20- to 40-nm gold nanoparticles (AuNP) with insufficient brightness as labels. To address this issue, we reported for the first time the successful synthesis of gold superparticles (GSP) by encapsulating numerous small AuNP into a ...
#1Wanida WonsawatH-Index: 7
Last. Wijitar Dungchai (King Mongkut's University of Technology Thonburi)H-Index: 25
view all 7 authors...
We first reported highly sensitive and rapid Salmonella Typhimurium (S. Typhimurium) quantitative method using a conductive immunosensor on a paper-based device (PAD). S. Typhimurium monoclonal antibodies (MA) were firstly immobilized on a paper-based device and then captured by S. Typhimurium. After an immunoreaction occurred on the device, the polyclonal antibody-colloidal gold conjugated (PA-AuNPs) was dropped to bind with S. Typhimurium. After a completed sandwich reaction occurred, a dark r...
#1Bo Zhang (JLU: Jilin University)H-Index: 53
Herein, we synthesized high-performance SiO2–core quantum dot (QD)–shell nanocomposites (SiO2@PEI-QDs) using the polyethyleneimine (PEI)-mediated adsorption method. Cationic PEI was used to form a positively charged interlayer on the SiO2 core, which achieved a dense adsorption of carboxylated QDs to form a shell of QDs and maintained a good dispersibility of the nanocomposite. The SiO2@PEI-QDs showed excellent stability and high luminescence, and served as high-performance fluorescent labels fo...
#1Tong Bu (NWAFU: Northwest A&F University)H-Index: 17
#2Xiaolin Yao (NWAFU: Northwest A&F University)H-Index: 10
Last. Daohong Zhang (NWAFU: Northwest A&F University)H-Index: 24
view all 9 authors...
Abstract As a rapid and facile means for foodborne bacteria detection in situ, lateral flow immunoassay (LFA) still has intrinsic limitations in the construction of the existing sandwich LFA format, e.g. screening difficulties of paired antibodies (Abs), poor stability of Ab probe, etc. Here, combined the strong affinity of antibiotic with the superior specificity of antibody molecules, a novel and robust LFA based on a dual recognition strategy and magnetic separation was designed to achieve sp...
#1Zilei Zhang (SJTU: Shanghai Jiao Tong University)H-Index: 6
#2Danlei Liu (SJTU: Shanghai Jiao Tong University)H-Index: 6
Last. Xianming Shi (SJTU: Shanghai Jiao Tong University)H-Index: 24
view all 6 authors...
Abstract DNA aptamers are promising candidates for bio-recognition assays due to their accumulation in bacterial cells. This feature can be used to identify specific pathogens in food samples. However, the conventional process of aptamer screening by randomly picking bacterial plate colonies for Sanger sequencing risks the elimination of superior sequences. In this study, we screened and evaluated all aptamers that we targeted to Salmonella Enteritidis ( S. Enteritidis). After 13 rounds of selec...
Cited By0
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.