Pingping Gao
Northeast Agricultural University
Colloidal goldDetection limitTransduction (genetics)AptamerMolecular biologyEnzymeChemistryConjugateNucleic acidRaw milkBrevundimonasAcinetobacterPathogenic bacteriaChryseobacteriumSalmonella entericaJanthinobacteriumMagnetic separationPsychrotrophic bacteriaEnterococcusLactococcusExonuclease IIIFood scienceChromatographyBacteriaEscherichia coliDNABiology
Publications 3
#1Yuwei Ren (NEAU: Northeast Agricultural University)H-Index: 1
#2Pingping Gao (NEAU: Northeast Agricultural University)H-Index: 1
Last. Yujun Jiang (NEAU: Northeast Agricultural University)H-Index: 8
view all 11 authors...
ABSTRACT Escherichia coli O157:H7 (E. coli O157:H7), one of the most widespread foodborne pathogens, can cause a series of diseases and even lead to death. In this study, a highly sensitive method was developed by combining aptamer-exonuclease III (Exo III)–assisted amplification with lateral flow assay (LFA) based on gold nanoparticles (AuNP). The compound of single-stranded (ss) DNA-anti-E. coli O157:H7 aptamer (ssDNA-aptamer) was formed by hybridization between designed target ssDNA and aptam...
#1Pingping GaoH-Index: 1
#2Lihan WangH-Index: 2
Last. Yujun JiangH-Index: 8
view all 10 authors...
In this paper, a novel and ultrasensitive lateral flow assay (LFA) based on aptamer–magnetic separation, and multifold Au nanoparticles (AuNPs) was developed for visual detecting Salmonella enterica ser. Typhimurium (S. Typhimurium). The method realized magnetic enrichment and signal transduction via magnetic separation and achieved signal amplification through hybridizing AuNPs–capture probes and AuNPs–amplification probes to form multifold AuNPs. Two different thiolated single-strand DNA (ssDN...
#1Xinyan Yang (NEAU: Northeast Agricultural University)H-Index: 5
#2Xiaojie Guo (NEAU: Northeast Agricultural University)H-Index: 4
Last. Chaoxin Man (NEAU: Northeast Agricultural University)H-Index: 8
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Abstract Psychrotrophic bacteria are the dominant organisms in refrigerated raw milk, which may have potentially adverse effects on the quality and shelf-life of dairy products. In this study, we investigated the concentration and microbial composition of psychrotrophic bacteria in raw milk from Heilongjiang Province (China) in winter and summer using traditional cultivation and single molecule real-time (SMRT) sequencing methods. The results showed that the mean counts of psychrotrophic bacteri...
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