Citrulline Recycling Supports Nitric Oxide Biosynthesis in Mouse PAM212 Keratinocytes

Nitric oxide synthases generate nitric oxide and citrulline from arginine using NADPH and molecular oxygen as co-substrates. In most mammalian cells, arginine is an essential amino acid and limiting extracellular supplies of this amino acid can reduce nitric oxide biosynthesis. This can occur during inflammation and injury where a variety of factors can reduce available arginine including high levels of tissue arginase. In cells and tissue...