Recombinant Expression of a Plant-Derived Dimeric Antifungal Peptide (DiSkh-AMP1) Joined by a Flexible Linker in Escherichia coli and Evaluation of Its Biological Activity In Vitro

DiSkh-AMP1, a novel dimeric antifungal peptide contained 65 amino acid residues was recombinant produced by a flexible linker to improve the antifungal activity of native Skh-AMP1 isolated from Satureja khuzistanica leaves. To discover a new system for expressing DiSkh-AMP1 in Escherichia coli BL21, the DiSkh-AMP1 gene was synthesized and inserted into pET28a vector in which fusion peptide (3.5 kDa) was used as a fusion partner and an N-terminal...