Digestion & fermentation characteristics of sulfated polysaccharides from Gracilaria chouae using two extraction methods in vitro and in vivo

Published on Jul 1, 2021in Food Research International4.972
· DOI :10.1016/J.FOODRES.2021.110406
Xiong Li3
Estimated H-index: 3
(SCUT: South China University of Technology),
Qingtong Xie (SCUT: South China University of Technology)+ 3 AuthorsSandra Pedisić12
Estimated H-index: 12
(University of Zagreb)
Source
Abstract
Abstract The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo. The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73×103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa. During stimulated gastrointestinal digestion in vitro, WGCP and CGCP were lightly degraded. However, the glycemic index (GI) of WGCP and CGCP were 17.7 and 36.12, respectively. After 24 h of fermentation in vitro, the pH values of CGLP in the fecal culture decreased from 6.89 to 4.82, similar to the inulin but significantly (p
📖 Papers frequently viewed together
4 Authors (Fulin Shi, ..., Yang Liu)
14 Citations
1 Citations
13 Citations
References55
Newest
Polysaccharides that contain many sugar monomers include starch and non-starch polysaccharides (NSPs) together with resistant starch (RS). Dietary polysaccharides are well known to have a wide range of biological benefits for bowel health. Gut microbiota and their fermentative products, short chain fatty acids (SCFA), which have recently been highlighted as metabolic regulators, are thought to mediate the function of dietary complex carbohydrates and bowel health. We discuss the influence of var...
6 CitationsSource
#1Xiong Li (SCUT: South China University of Technology)H-Index: 3
#2Huang Shiming (SCUT: South China University of Technology)H-Index: 2
Last. Jin-hua Piao (SCUT: South China University of Technology)H-Index: 14
view all 9 authors...
A sulfated polysaccharide extracted from Gracilaria lemaneiformis (GLP) with a prominent effect in regulating lipid metabolism was isolated. The molecular weight was 31.5 kDa and it was composed mainly of galactose, glucose and xylose. Fourier-transform infrared (FT-IR) spectrum and nuclear magnetic resonance (NMR) analysis suggested that GLP was composed of the following repeating unit: [3-β-Gal-4(OSO3)-1→4-α-3,6-anhydrogal-2(OSO3)-1→]. GLP could significantly decrease serum total cholesterol, ...
1 CitationsSource
#1Xiaoyong Chen (SCUT: South China University of Technology)H-Index: 2
#2Ruifen ZhangH-Index: 7
Last. Kseniya Hileuskaya (NASB: National Academy of Sciences of Belarus)H-Index: 5
view all 7 authors...
Abstract The depolymerization effect of UV/H2O2 on the polysaccharides from Sargassum fusiforme (PSF), a brown algae, were studied. The structural changes of PSF before and after UV/H2O2 treatment were analyzed, and molecular weight changes during in vitro digestion were determined. Results indicated that the molecular weight of PSF was reduced from ∼289 to ∼12.6 kDa within 2 h with UV/150 mmol/L H2O2, and the depolymerization effect of UV/H2O2 was significantly higher than that of UV or H2O2 al...
13 CitationsSource
#1Qing-Mei Liu (JMU: Jimei University)H-Index: 12
#2Ya-Fen Zhang (JMU: Jimei University)H-Index: 4
Last. Guang-Ming Liu (JMU: Jimei University)H-Index: 25
view all 10 authors...
Abstract Sulfated oligosaccharide of Gracilaria lemaneiformis (GLSO) was prepared from sulfated polysaccharides which possessed antiallergic activity by degradation with high temperature and pressure combined with vitamin C treatment. The present study demonstrated that GLSO could attenuate food anaphylaxis, and inhibit the production of immunoglobulin E, histamine, and related cytokines in both prevention and therapy ovalbumin-induced mice model. Additionally, the gut microbiota analysis reveal...
7 CitationsSource
#1Yuxin Xu (Dalian Polytechnic University)H-Index: 2
#2Chunqing Ai (Dalian Polytechnic University)H-Index: 11
Last. Shuang Song (Dalian Polytechnic University)H-Index: 17
view all 7 authors...
Polysaccharides from Gracilaria lemaneiformis (GLPs) was shown to reduce high fat diet (HFD)-induced metabolic syndrome (MS), but its high molecular weight (MW) causes lots of barriers to its application in foods as a functional ingredient. In this study, oligosaccharides derived from GLPs (D-GLPs) were prepared, and its effect on HFD-induced MS and inflammation was evaluated in mice. It revealed that D-GLPs reduced fat accumulation, lipid disorders and inflammation in HFD-fed mice accompanied w...
2 CitationsSource
#1Rui Han (SCUT: South China University of Technology)H-Index: 2
#2Daorui PangH-Index: 5
Last. Viktoryia Kulikouskaya (NASB: National Academy of Sciences of Belarus)H-Index: 7
view all 6 authors...
Abstract The study explored the changes of a sulfated polysaccharide (SP) from Gracilaria Lemaneiformis during simulated human digestive system. The results showed SP was scarcely degraded without significant difference. Moreover, the results of fermentation in vitro indicated 53.7% of SP were utilized by gut microbiota with the molecular weight decreasing by 59.41% at 48 h. Meanwhile, 50.07 ± 2.82 mM of short chain fatty acids were produced in culture medium. Besides, in contrast with the regul...
16 CitationsSource
#1Ying Rui (NAU: Nanjing Agricultural University)H-Index: 3
#2Peng Wan (NAU: Nanjing Agricultural University)H-Index: 14
Last. Zhonghua Liu (HAU: Hunan Agricultural University)H-Index: 19
view all 7 authors...
Abstract Fuzhuan brick tea is characterized in unique “golden flower” of Aspergillus cristatus. However, little information is available for intra- and extra-cellular polysaccharides from A. cristatus (IPS and EPS, respectively). Therefore, simulated digestion and fermentation in vitro were carried out to investigate whether IPS and EPS could be broken down in digestive system. Furthermore, their interactions with gut microbiota were investigated. It was found that IPS and EPS were utilized by g...
8 CitationsSource
#1Xiaomei Wang (NBU: Ningbo University)H-Index: 2
#1Xiaomei Wang (NBU: Ningbo University)H-Index: 5
Last. Nianjun Xu (NBU: Ningbo University)H-Index: 9
view all 6 authors...
Abstract The anti-aging activity of marine macroalgae Gracilaria lemaneiformis polysaccharide (GP) on Caenorhabditis elegans was evaluated by observing the lifespan, reproduction, pharyngeal pumping and stress response of worms. Moreover, quantitative fluorescence of polyglutamic acid and nuclear localization of DAF-16 were observed. The results showed that GP treatment enhanced the mean lifespan by over 16.47% and significantly increased the reproduction duration of worm in the high dose group ...
9 CitationsSource
#1Huang Lixin (NCU: Nanchang University)
#1Lixin Huang (NCU: Nanchang University)H-Index: 7
Last. Jianhua Xie (NCU: Nanchang University)H-Index: 35
view all 4 authors...
Abstract Background Polysaccharides are natural macromolecular polymers that are widely distributed in various food resources and have attracted much attention due to their significant bioactivities. Sulfated polysaccharides refer to polysaccharides containing sulfate groups on sugar units. A large number of studies have characterized and evaluated the biological relevance of sulfated polysaccharides, which shows great potential in terms of immunological activity. Scope and approach Through a cr...
32 CitationsSource
#1Qingying Fang (NCU: Nanchang University)H-Index: 3
#2Jielun Hu (NCU: Nanchang University)H-Index: 23
Last. Shaoping Nie (NCU: Nanchang University)H-Index: 65
view all 4 authors...
Abstract Background As an important role of host homeostasis, the glycometabolism was closely related to the process and metabolic pathways of carbohydrates such as glycolysis, hexosamine biosynthetic pathway, and pentose phosphate in the host. Disorders of glycometabolism result in a series of metabolic diseases, such as diabetes, obesity, hypoglycemia, and etc. Scope and approach Polysaccharides, which were widely existing in daily diet and cannot be directly digested by gastrointestinal enzym...
20 CitationsSource
Cited By0
Newest