Discrimination and characterization of Panax polysaccharides by 2D COS-IR spectroscopy with chemometrics.

Published on Apr 24, 2021in International Journal of Biological Macromolecules5.162
· DOI :10.1016/J.IJBIOMAC.2021.04.124
Yan-Li Lü (Heilongjiang University of Chinese Medicine), Jun Liang12
Estimated H-index: 12
(Heilongjiang University of Chinese Medicine)
+ 2 AuthorsYong-Gang Xia20
Estimated H-index: 20
(Heilongjiang University of Chinese Medicine)
Abstract In this study, a novel two-dimensional correlation infrared spectroscopy (2DCOS-IR) is presented to rapidly characterize and discriminate polysaccharides in Panax ginseng (PGP), P. notoginseng (PNP), and P. quinquefolius (PQP) using attenuated total reflection Fourier transform infrared spectroscopy–based on single-characteristic temperature as the external disturbance (2D-sATR-FTIR). Compared with two existing 2DCOS-IR methods based on gradient heating pathways using KBr pellet (100 min; 2D-KBr-FTIR) and attenuated total reflection (30 min; 2D-gATR-FTIR), the new procedure took an average of just 2 min to finish a sample measurement, which resolved previously tedious and time-consuming dilemmas. It offered advantages in the quality evaluation of natural polysaccharides and featured nondestructive, high-throughput, and high-efficiency characteristics. An intuitive analysis of the 2D-sATR-FTIR demonstrated that PNP was first identified because it had fewer auto-peaks. Posteriorly, PGP and PQP were distinguished according to the ratio of the auto-peaks 6 and 9, with the former greater than 1 and the latter less than 1. Furthermore, characteristic auto-peaks 1, 5, and 6 were unambiguously determined as Quality-markers using PCA and PLS-DA for visualized identifications. LDA was successfully used to establish a predictive model of the PGP, PNP, and PQP based on the positions and intensity of these three characteristic auto-peaks.
#1Fengxiang Zhang (WMU: Weifang Medical University)H-Index: 3
#2Shaojian Tang (WMU: Weifang Medical University)H-Index: 2
Last. Peng Xue (WMU: Weifang Medical University)H-Index: 4
view all 7 authors...
Abstract Background Ginsenosides, which have strong biological activities, can be divided into polar or less polar ginsenosides. Methods This study evaluated the phytochemical diversity of the saponins in Panax ginseng (PG) root, American ginseng (AG) root, and Panax notoginseng (NG) root; the stem-leaves from Panax ginseng (SPG) root, American ginseng (SAG) root, and Panax notoginseng (SNG) root as well as the saponins obtained following heating and acidification (TPG, TAG, TNG, TSPG, TSAG, and...
9 CitationsSource
#1Mi Kaythi Chan (Northeast Normal University)H-Index: 1
#2Yang Yu (Northeast Normal University)H-Index: 2
Last. Lin Sun (Northeast Normal University)H-Index: 14
view all 7 authors...
Abstract Panax notoginseng is a widely used traditional Chinese medicine and has extensive pharmacological effects. In this work, water-soluble polysaccharides from Panax notoginseng were isolated and fractionated. One starch-like polysaccharide (PNPN) and six pectin fractions (PNPA-1A, PNPA-1B, PNPA-2A, PNPA-2B, PNPA-3A and PNPA-3B) were obtained. Monosaccharide composition, enzymatic hydrolysis, nuclear magnetic resonance and methylation analysis were combined to characterize their structures....
4 CitationsSource
Abstract In order to identify real and fake Panax notoginseng samples, the high performance liquid chromatography (HPLC) was used to analyze P. notoginseng samples of non-volatile characteristic components in P. notoginseng powder samples with 10%, 30%, 50% ratio, combined with principal component analysis - mahalanobis distance (PCA-MD). The results showed that: (1) The PCA analysis showed that mahalanobis with different stem and leaf powder were divided into four categories, and the gravity ce...
2 CitationsSource
#1Xiaodong Yang (SWU: Southwest University)H-Index: 4
#2Jie Song (SWU: Southwest University)H-Index: 4
Last. Guanglin Li (SWU: Southwest University)H-Index: 6
view all 7 authors...
Abstract Panax notoginseng (P. notoginseng) is frequently targeted for adulteration with lower-grade P. notoginseng because of its high price. This paper presents a novel method to improve the identification ability of adulterants using spectroscopic techniques and chemometrics. P. notoginseng powder of different grades were blended at different percentages (0–100%), in which the minimum blend ratio was 0.5%. NIR (12,497–4000 cm−1) and FT-MIR (4000–400 cm−1) spectra of samples were acquired. The...
4 CitationsSource
#1Jing Li (KTH: Royal Institute of Technology)H-Index: 4
#2Damao Wang (KTH: Royal Institute of Technology)H-Index: 9
Last. Yves S.Y. Hsieh (KTH: Royal Institute of Technology)H-Index: 13
view all 8 authors...
Abstract Ginseng marc is a major by-product of the ginseng industry currently used as animal feed or fertilizer. This fibrous, insoluble waste stream is rich in cell wall polysaccharides and therefore a potential source of ingredients for functional food with health-promoting properties. However, the extraction of these polysaccharides has proved problematic and their exact composition remains unknown. Here we have analysed the composition, structure and biological activity of polysaccharides fr...
8 CitationsSource
#1Chandra P. Sharma (Wildlife Institute of India)H-Index: 56
#2Sweety Sharma (Punjabi University)H-Index: 7
Last. Rajinder Singh (Punjabi University)H-Index: 8
view all 4 authors...
Abstract Differentiation and identification of Royal Bengal Tiger (Panthera tigris tigris) and Indian Leopard (Panthera pardus fusca) claws is a challenging task in wildlife forensics, due to similarity in their morphology, anatomy and chemical compositions as both the species are closely related to each other genetically. ATR-FTIR spectroscopy, which offers a non-destructive and safe alternative technique to other conventional methods, has been employed in the present work to differentiate claw...
6 CitationsSource
#1Bin Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 2
#2Chongning Lv (SPU: Shenyang Pharmaceutical University)H-Index: 9
Last. Jincai Lu (SPU: Shenyang Pharmaceutical University)H-Index: 14
view all 3 authors...
Abstract Ginseng Radix et Rhizome, which originated from Panax ginseng C. A. Meyer, has been used as traditional medicine in Asian for >2000 years. It is famous for its healthcare and therapeutic effects. Phytochemistry studies revealed that the major components of P. ginseng are: ginsenosides, polysaccharides, small peptides and essential oil. Modern research has proved that polysaccharide is one of the most important active ingredients in P. ginseng, which also takes up the largest slice of th...
27 CitationsSource
#1Liang ShenH-Index: 4
#2Xi-Wen LiH-Index: 2
Last. Shilin ChenH-Index: 64
view all 9 authors...
ABSTRACT American ginseng (Panax quinquefolius L.) is a well-known Asian traditional herbal medicine with a large market demand. The plant is native to eastern North America, and its main producing areas worldwide are decreasing due to continuous cropping obstacles and environmental changes. Therefore, the identification of maximum similarities of new ecological distribution of P. quinquefolius, and prediction of its response to climate change in the future are necessary for plant introduction a...
1 CitationsSource
#1Nur Cebi (YTU: Yıldız Technical University)H-Index: 7
#2Canan Ekinci Dogan (Scientific and Technological Research Council of Turkey)H-Index: 6
Last. Osman Sagdic (YTU: Yıldız Technical University)H-Index: 43
view all 6 authors...
Abstract Gelatin is widely used in gummy candies because of its unique functional properties. Generally, porcine and bovine gelatins are used in the food industry. FTIR-ATR combined with chemometrics analysis such as hierarchical cluster analysis (HCA) (OPUS Version 7.2 software), principal component analysis (PCA) (OPUS Version 7.2 software) and partial least squares-discriminant analysis (PLS-DA) (Matlab R2017b) were used for classification and discrimination of gelatin gummy candies related t...
18 CitationsSource
#1Lan-fen YangH-Index: 1
#2Fang Ma (THU: Tsinghua University)H-Index: 7
Last. Su-Qin Sun (THU: Tsinghua University)H-Index: 25
view all 4 authors...
Abstract Wild and cultivated Paridis Rhizoma (PR) were analyzed and characterized systematically by integral analysis method and two dimensional correlation infrared spectroscopy, both of which have been successfully utilized in the analysis of complex mixtures. According to their infrared spectra, peaks clusters at around 1642 and 1022 cm−1 could be considered as the key factors to discriminate them. Besides, significant differences emerged in their second derivative spectra, particularly the p...
15 CitationsSource
Cited By0