Jun Liang
Heilongjiang University of Chinese Medicine
PolysaccharideAnalytical chemistryMonosaccharideRepeatabilityMass spectrometryTriple quadrupole mass spectrometerHigh-performance liquid chromatographyChemistryElectrosprayGlycosideGas chromatography–mass spectrometryArabinoseForsythiaGlucuronic acidDerivatizationForsythia suspensaPhillyrinTriterpeneEphedra sinicaAralia elataPhenylethanoidElectrospray ionizationChromatographyExtraction (chemistry)Selected reaction monitoringStereochemistryQuantitative analysis (chemistry)
43Publications
12H-index
423Citations
Publications 42
Newest
#1Yan-Li Lü (Heilongjiang University of Chinese Medicine)
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
view all 5 authors...
Abstract In this study, a novel two-dimensional correlation infrared spectroscopy (2DCOS-IR) is presented to rapidly characterize and discriminate polysaccharides in Panax ginseng (PGP), P. notoginseng (PNP), and P. quinquefolius (PQP) using attenuated total reflection Fourier transform infrared spectroscopy–based on single-characteristic temperature as the external disturbance (2D-sATR-FTIR). Compared with two existing 2DCOS-IR methods based on gradient heating pathways using KBr pellet (100 mi...
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#1Li-Shi Yu (Heilongjiang University of Chinese Medicine)H-Index: 2
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
view all 5 authors...
Abstract In this study, an uncommon enzymatic-fingerprinting workflow, was proposed for characterization and discrimination of mushroom polysaccharides (MPs) by hydrophilic interaction liquid chromatography-negative electrospray mass spectrometry (HILIC-ESI–MS). Firstly, the HILIC-ESI–MS was used to screen and identify the enzymatic digestion products of MPs using HILIC-Orbitrap based on full scan and MS/MS modes. Secondly, a targeted structural-fingerprinting of polysaccharides (SFP) was built ...
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#1Yu Shen (Heilongjiang University of Chinese Medicine)H-Index: 1
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
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Two novel arabinose- and galactose-rich pectic polysaccharides, AELP-B5 (Mw, 4.25 × 104 g/mol) and B6 (Mw, 1.56 × 104 g/mol), were rapidly obtained from the leaves of Aralia elata (Miq.) Seem. with anion resin and sequenced ultrafiltration membrane columns. The structural backbone and branched chains of AELP-B5 and B6 were preliminarily elucidated by mild acid hydrolysis with HILIC-ESI--MS/MS. The planar structures and spatial configurations were further identified using UPLC-QDa and GC-MS for c...
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#1Chen-Xue Li (Heilongjiang University of Chinese Medicine)H-Index: 1
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
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Abstract Plant saponins are important natural product with biologically active. However, the metabolism of these compounds has rarely been studied due to their low bioavailability and the complexity of their metabolite structures. In this study, ultra-performance liquid chromatography/Fusion Lumos Orbitrap mass spectrometry was used to analyze the metabolites of hederasaponin B in vivo, and its possible metabolic pathways were proposed. After oral administration of the parent drug, 47 metabolite...
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#1Ye Li (Heilongjiang University of Chinese Medicine)H-Index: 2
#2Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
Last. Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
view all 5 authors...
Abstract A novel ultra-high-performance liquid chromatography-electrospray mass spectrometry (UHPLC-ESI-MS) method was proposed for simultaneous determination of neutral and acidic monosaccharaides by employing acetylation derivatization that is specialized for gas chromatography-mass spectrometry (GC-MS) analyses. The UHPLC-ESI-MS was carried out in a positive ionization mode with selective ion monitoring (SIM) of sodium adducts at m/z 385 (Ara, Xyl and Rib), 399 (Rha and Fuc), 443 (Glc, Gal an...
1 CitationsSource
#1Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
#2Rong-Jian Zhu (Heilongjiang University of Chinese Medicine)H-Index: 1
Last. Hai-Xue Kuang (Heilongjiang University of Chinese Medicine)H-Index: 26
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Abstract In this study, a pectin polysaccharide, AER-A3 (Mw 1.12 × 105 g/mol), was isolated and purified from the root bark of Aralia elata by ion exchange and gel-filtration chromatography. Its planar structure was investigated in combination with UPLC-ESI-MS, FT-IR, HILIC-ESI−-MS/MS, GC–MS and NMR techniques. The main chain of AER-A3 was unambiguously determined to be smooth region and hairy region with a chain length ratio of 1:1 characterized by occurrence of (1 → 2)-α-Rhap, (1 → 2,3)-α-Rhap...
7 CitationsSource
#1Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
#2Xin Li (Heilongjiang University of Chinese Medicine)H-Index: 1
Last. Hai-Xue Kuang (Heilongjiang University of Chinese Medicine)H-Index: 26
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Abstract In this paper, a sequential gas-liquid chromatography and mass spectrometry route was proposed for characterization of polysaccharides in Panax ginseng (PG), P. notoginseng (PN), and P. quinquefolius (PQ). Due to the reflection of stepped structure parameters, the resulting integrative profiles were tentatively defined as structural-fingerprinting of polysaccharides (SFP) with monosaccharide compositional fingerprinting (MCF), Smith degradation and non-degradation fingerprinting (SDF an...
5 CitationsSource
#1Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
#2Yong-Xin Huang (Heilongjiang University of Chinese Medicine)H-Index: 1
Last. Hai-Xue Kuang (Heilongjiang University of Chinese Medicine)H-Index: 26
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Abstract Two pectin-polysaccharides, ASP-B2 (Mw, 5.32 kDa) and B3 (Mw, 30.51 kDa), were obtained from Acanthopanax senticosus leaves. An HILIC-UPLC-ESI−-HCD-MS/MS technique was introduced for structural elucidations of skeletal structure of pectin-polysaccharides in addition to empolying SEC-MALLS, GC–MS, UPLC-ESI+-MS, methylation analysis, FT-IR and NMR methods. Skeletal structure of ASP-B2 was characterized by combining HG smooth region and RG hairy region with a 2:1 chain length ratio, wheres...
7 CitationsSource
#1Yong-Gang Xia (Heilongjiang University of Chinese Medicine)H-Index: 20
#2Si-Miao Yu (Heilongjiang University of Chinese Medicine)H-Index: 2
Last. Hai-Xue Kuang (Heilongjiang University of Chinese Medicine)H-Index: 26
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Abstract A generic strategy based on chemical fingerprinting is proposed for the differentiation of polysaccharides from Astragalus membranaceus (AEP) and A. mongholicus (AOP), using multiple chromatographic and mass spectrometric techniques. Complete and mild acid hydrolysis and Smith degradation were employed for the depolymerization of polysaccharides. The corresponding digested products were efficiently separated and detected using GC–MS, HILIC-ELSD and HR-ESI−-MS. The resulting bottom-up fi...
4 CitationsSource
#1Jun Liang (Heilongjiang University of Chinese Medicine)H-Index: 12
#2Xin-Dong Guo (Heilongjiang University of Chinese Medicine)H-Index: 4
Last. Fang Han (Heilongjiang University of Chinese Medicine)H-Index: 1
view all 3 authors...
Nowadays, the analysis of the multi-components in Chinese patent medicine prescriptions is being paid more attention. Therefore, in this study for the first time, a simple, rapid ultrahigh performance liquid chromatography–electrospray ionization tandem mass spectrometry (UHPLC-ESI-MS/MS) method was established for simultaneous determination of 18 active compounds in a Chinese patent medicine of Hu Gan tablets (HGT) from different pharmaceutical factories in China. This task has met great emergi...
1 CitationsSource