Characterization of a novel GH10 xylanase with a carbohydrate binding module from Aspergillus sulphureus and its synergistic hydrolysis activity with cellulase.
A study was carried out to investigate the characterization of a novel Aspergillus sulphureus JCM01963 xylanase (AS-xyn10A) with a carbohydrate binding module (CBM) and its application in degrading alkali pretreated corncob, rapeseed meal and corn stover alone and in combination with a commercial cellulase. In this study, the 3D structure of AS-xyn10A, which contained a CBM at C-terminal. AS-xyn10A and its CBM-truncated variant (AS-xyn10A-dC) was codon-optimized and over-expressed in Komagaella phaffii X-33 (syn. Pichia pastoris) and characterized with optimal condition at 70 °C and pH 5.0, respectively. AS-xyn10A displayed high activity to xylan extracted from corn stover, corncob, and rapeseed meal. The concentration of hydrolyzed xylo-oligosaccharides (XOSs) reached 1592.26 μg/mL, 1149.92 μg/mL, and 621.86 μg/mL, respectively. Xylobiose was the main product (~70%) in the hydrolysis mixture. AS-xyn10A significantly synergized with cellulase to improve the hydrolysis efficiency of corn stover, corncob, and rapeseed meal to glucose. The degree of synergy (DS) was 1.32, 1.31, and 1.30, respectively. Simultaneously, XOSs hydrolyzed with AS-xyn10A and cellulase was improved by 46.48%, 66.13% and 141.45%, respectively. In addition, CBM variant decreased the yields of xylo-oligosaccharide and glucose in rapeseed meal degradation. This study provided a novel GH10 endo-xylanase, which has potential applications in hydrolysis of biomass.
#1Anil Kumar Singh(IITR: Indian Institute of Toxicology Research)H-Index: 3
#1Anil Kumar Singh(AcSIR: Academy of Scientific and Innovative Research)H-Index: 3
Last. Abhay Raj(AcSIR: Academy of Scientific and Innovative Research)H-Index: 1
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Abstract Lignin peroxidase (LiP) seems to be a catalyst for cleaving high-redox potential non-phenolic compounds with an oxidative cleavage of C C and C O C bonds. LiP has been picked to seek a practical and cost-effective alternative to the sustainable mitigation of diverse environmental contaminants. LiP has been an outstanding tool for catalytic cleaning and efficient mitigation of environmental pollutants, including lignin, lignin derivatives, dyes, endocrine-disrupting compounds (EDCs), and...
Last. Bijender Singh(Central University, India)H-Index: 25
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Abstract Baking is a universal process for the preparation of baked products like, bread, biscuits, pastries, cookies, crackers, pies, and others. Although usage of enzymes in bakery is quite old but recent developments in basic biology, biochemistry, advancements in technological approaches, and discovery of new/novel enzymes, has revolutionized the application potential of enzymes in a variety of food industries including the bakery. World over people want their foods to be free of chemical pr...
#2Carolina Velasco-Ayala(Tec: Monterrey Institute of Technology and Higher Education)H-Index: 1
Last. Danay Carrillo-Nieves(Tec: Monterrey Institute of Technology and Higher Education)H-Index: 8
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Abstract Lignocellulosic material has drawn significant attention among the scientific community due to its year-round availability as a renewable resource for industrial consumption. Being an economic substrate alternative, various industries are reevaluating processes to incorporate derived compounds from these materials. Varieties of fungi and bacteria have the ability to depolymerize lignocellulosic biomass by synthesizing degrading enzymes. Owing to catalytic activity stability and high yie...
Pectin-rich agro-industrial residues are feedstocks with potential for sustainable biorefineries. They are generated in high amounts worldwide from the industrial processing of fruits and vegetables. The challenges posed to the industrial implementation of efficient bioprocesses are however manyfold and thoroughly discussed in this review paper, mainly at the biological level. The most important yeast cell factory platform for advanced biorefineries is currently Saccharomyces cerevisiae, but thi...
Last. Roberto Parra-Saldívar(Tec: Monterrey Institute of Technology and Higher Education)H-Index: 25
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The use of lignocellulosic wastes reduces dependence on fossil fuel resources, contributes to sustainable waste management, and reinforces the circular economy model of continual use of resources. Typically, the second generation of bioethanol production involves several steps to transform lignocellulosic material into bioethanol. The more complicated step of the overall process is to define a tailor-made to each lignocellulosic material available with a wide variety of complex structures of the...
Last. Junhua Zhang(NWAFU: Northwest A&F University)H-Index: 22
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Abstract The severe pretreatment of poplar makes xylan difficult to utilize efficiently. In this work, poplar was pretreated by hydrogen peroxide–acetic acid (HPAC) with H2SO4 as catalyst to remove lignin, and the solid residues were used to produce xylooligosaccharides (XOS) and monosaccharides by two-step xylanase and cellulase treatment. The results indicated that higher H2SO4 concentrations in the HPAC pretreatment of poplar afforded stronger lignin removal ability. An increased XOS yield of...
#1Qiuhua Wu(BTBU: Beijing Technology and Business University)H-Index: 5
#2Guangsen Fan(BTBU: Beijing Technology and Business University)H-Index: 9
Last. Xiuting Li(BTBU: Beijing Technology and Business University)H-Index: 15
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Abstract The thermostable recombinant xylanase, T-XynC(122)C(166), was investigated for its biochemical characteristics and capacity to produce xylooligosaccharides from corncobs pretreated through auto-hydrolysis. T-XynC(122)C(166) showed optimal activity at pH 6.5 and 75 °C, and is an acidophilic and thermostable xylanase with a Km of 6.08 mg/mL towards beechwood xylan. Hydrolysis of xylan into xylooligosaccharides was through an endohydrolytic mode of action. Although xylotriose and xylobiose...
Xylanase is one of the most extensively used biocatalysts for biomass degradation. However, its low catalytic efficiency and poor thermostability limit its applications. Therefore, improving the properties of xylanases to enable synergistic degradation of lignocellulosic biomass with cellulase is of considerable significance in the field of bioenergy. Using fragment replacement, we improved the catalytic performance and thermostability of a GH10 xylanase, XylE. Of the ten hybrid enzymes obtained...
Abstract A novel carbohydrate binding module (CBM) was identified in a β-1,3-xylanase from Flammeovirga pacifica, which showed only 25.0% sequence identity with the reported CBMs with the coverage of 36.4%. To verify its function, a truncated β-1,3-xylanase (Xy13088-T) and a carbohydrate binding module (CBM3088) were expressed and purified. The thermostability and catalytic efficiency of the Xy13088-T declined significantly when compared with the full-length one, with the decreasing of the half-...