Porous biodegradable scaffolds provide a physical substrate for cells allowing them to attach, proliferate and guide the formation of new tissues. A variety of techniques have been developed to fabricate tissue engineering (TE) scaffolds, among them the most relevant is the thermally-induced phase separation (TIPS). This technique has been widely used in recent years to fabricate three-dimensional (3D) TE scaffolds. Low production cost, simple experimental procedure and easy processability together with the capability to produce highly porous scaffolds with controllable architecture justify the popularity of TIPS. This paper provides a general overview of the TIPS methodology applied for the preparation of 3D porous TE scaffolds. The recent advances in the fabrication of porous scaffolds through this technique, in terms of technology and material selection, have been reviewed. In addition, how properties can be effectively modified to serve as ideal substrates for specific target cells has been specifically addressed. Additionally, examples are offered with respect to changes of TIPS procedure parameters, the combination of TIPS with other techniques and innovations in polymer or filler selection.
Last. Jinzhong Zhao(SJTU: Shanghai Jiao Tong University)H-Index: 21
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The postoperative retear rate of direct repair of massive rotator cuff tear has risen up to 40%, due to the dissatisfied tendon-to-bone healing and poor regenerative potential of remnant rotator cu...
#1Esperanza Díaz(UPV/EHU: University of the Basque Country)H-Index: 10
#2Jon Aresti(UPV/EHU: University of the Basque Country)H-Index: 1
Last. Joseba León(UPV/EHU: University of the Basque Country)H-Index: 2
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The fabrication of biodegradable magnetic poly(caprolactone)(PCL)/nanohydroxyapatite (nHA)/multiwalled carbon nanotube (MWCNT) composite scaffolds by thermally induced phase separation (TIPS) for b...
#1Reza Zeinali(IAU: Islamic Azad University)H-Index: 1
Last. Jordi Puiggalí(UPC: Polytechnic University of Catalonia)H-Index: 39
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Thermally induced phase separation followed by freeze drying has been used to prepare biodegradable and biocompatible scaffolds with interconnected 3D microporous structures from poly(hydroxybutyrate-co-hydroxyvalerate) (PHBV) copolymers containing 5 and 12 wt % of 3-hydroxyvalerate (HV). Solutions of PHBV in 1,4-dioxane, underwent phase separation by cooling under two different thermal gradients (at −25 °C and −5 °C). The cloud point and crystallization temperature of the polymer solutions were...
#1Hadi Samadian(Kermanshah University of Medical Sciences)H-Index: 18
#2Saeed Farzamfar(Tehran University of Medical Sciences)H-Index: 14
Last. Majid Salehi(Shahroud University of Medical Sciences)H-Index: 19
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The focus of the current study was to develop a functional and bioactive scaffold through the combination of 3D polylactic acid (PLA)/polycaprolactone (PCL) with gelatin nanofibers (GNFs) and Taurine (Tau) for bone defect regeneration. GNFs were fabricated via electrospinning dispersed in PLA/PCL polymer solution, Tau with different concentrations was added, and the polymer solution converted into a 3D and porous scaffold via the thermally-induced phase separation technique. The characterization...
Last. Hongli Sun(USD: University of South Dakota)H-Index: 22
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Three-dimensional (3D) scaffolds with interconnected, hierarchically structured pores, and biomimetic nanostructures are desirable for tissue engineering, where preparation with a facile and biocompatible strategy remains challenging. In the present work, an innovative porous microspheres-aggregated 3D PCL scaffold with macropores, micropores, and nanofibrous-like structures was fabricated through a one-pot thermally induced phase separation (TIPS) method without the use of any porogen or specif...
Last. Lorenzo Moroni(UM: Maastricht University)H-Index: 55
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Controlling angiogenesis within tissue engineered constructs remains a critical challenge, especially with regard to the guidance of pre-vascular network formation. Here, we aimed to regulate angiogenesis on a self-assembled honeycomb nanofibrous scaffold. Scaffolds with honeycombs patterns have several desirable properties for tissue engineering, including large surface area, high structural stability and good permeability. Furthermore, the honeycomb pattern resembles early vascular network for...
#1Esperanza Díaz(UPV/EHU: University of the Basque Country)H-Index: 10
#2Julen Martín(UPV/EHU: University of the Basque Country)H-Index: 1
Last. Joseba León(UPV/EHU: University of the Basque Country)H-Index: 2
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Poly(L-lactide (PLLA), nano-hydroxyapatite (nHA) and multi-walled carbon nanotube (MWCNTs) biodegradable scaffolds were produced through thermally induced phase separation (TIPS) and ultrasonically...
Last. Binghong Luo(JNU: Jinan University)H-Index: 17
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Abstract We present here a porous poly (L-lactide)/chitosan (PLLA/CS) composite scaffold with micro/nano-fiber hierarchical structure that was innovatively fabricated by combining 3D printing and thermally induced phase separation technology. Moreover, bioactive quercetin (Qu) was chosen to further functionalize the PLLA/CS scaffold with polydopamine (PDA) layer. The final results showed that the hydrophilicity and mechanical properties of the resulting PLLA/CS-D/Qu scaffold were obviously super...
#1Qingqing Yao(WMU: Wenzhou Medical College)H-Index: 8
#2Kirby Fuglsby(USD: University of South Dakota)H-Index: 3
Last. Hongli Sun(UI: University of Iowa)H-Index: 22
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Developing a biomaterial that can promote osteoblastic differentiation, thereby reducing the needs of exogenous osteogenic factors for large bone repair has been a significant and long-term technical hurdle. In this study, we developed an innovative nanoclay (Nanosilicate, NS)-functionalized 3D gelatin nanofibrous scaffold (GF/NS) through thermally induced phase separation method together with particle leaching technique (TIPSP particularly, the strong binding ability to pro-osteoblastic factors...
#1Chi Zhang(SYSU: Sun Yat-sen University)H-Index: 4
#2Peng Dong(SYSU: Sun Yat-sen University)H-Index: 3
Last. Daping Quan(SYSU: Sun Yat-sen University)H-Index: 17
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Abstract The nanofibrous ultrastructure of native extracellular matrix (ECM) provides topological cues for specific cellular behavior, therefore, serves as a physical template for bio-scaffolds design and fabrication. Poly(γ-benzyl-L-glutamate) (PBLG) is a commonly used polypeptide, which often self-assembles into nanofibers. However, the slow degradation of PBLG limits its application in tissue engineering. Herein, poly(D,L-lactide)-b-poly(γ-benzyl-L-glutamate) (PDLLA-b-PBLG) was successfully s...