Transcription factor EB (TFEB)-mediated autophagy protects bovine mammary epithelial cells against H 2 O 2-induced oxidative damage in vitro

Published on Mar 9, 2021in Journal of animal science and biotechnology5.032
· DOI :10.1186/S40104-021-00561-7
Xudong Sun8
Estimated H-index: 8
Renxu Chang2
Estimated H-index: 2
(HAU: Hunan Agricultural University)
+ 8 AuthorsChuang Xu8
Estimated H-index: 8
BACKGROUND Bovine mammary epithelial cells after calving undergo serious metabolic challenges and oxidative stress both of which could compromise autophagy. Transcription factor EB (TFEB)-mediated autophagy is an important cytoprotective mechanism against oxidative stress. However, effects of TFEB-mediated autophagy on the oxidative stress of bovine mammary epithelial cells remain unknown. Therefore, the main aim of the study was to investigate the role of TFEB-mediated autophagy in bovine mammary epithelial cells experiencing oxidative stress. RESULTS H2O2 challenge of the bovine mammary epithelial cell MAC-T increased protein abundance of LC3-II, increased number of autophagosomes and autolysosomes while decreased protein abundance of p62. Inhibition of autophagy via bafilomycin A1 aggravated H2O2-induced reactive oxygen species (ROS) accumulation and apoptosis in MAC-T cells. Furthermore, H2O2 treatment triggered the translocation of TFEB into the nucleus. Knockdown of TFEB by siRNA reversed the effect of H2O2 on protein abundance of LC3-II and p62 as well as the number of autophagosomes and autolysosomes. Overexpression of TFEB activated autophagy and attenuated H2O2-induced ROS accumulation. Furthermore, TFEB overexpression attenuated H2O2-induced apoptosis by downregulating the caspase apoptotic pathway. CONCLUSIONS Our results indicate that activation of TFEB mediated autophagy alleviates H2O2-induced oxidative damage by reducing ROS accumulation and inhibiting caspase-dependent apoptosis.
#1Xinwei Li (JLU: Jilin University)H-Index: 34
#2Guojin Li (JLU: Jilin University)H-Index: 3
Last. Guowen Liu (JLU: Jilin University)H-Index: 27
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ABSTRACT Hyperketonemia is a metabolic disease in dairy cows, associated with negative nutrition balance (NNB) induced by low dry matter intake (DMI) and increased nutrient requirements. Hyperketonemia could induce metabolic stress, which might indirectly affect mammary tissue. Autophagy is a highly conserved physiological process that results in the turnover of intracellular material, and is involved in maintaining cellular homeostasis under the challenge of metabolic stress induced by NNB. The...
#1Xudong SunH-Index: 8
#2Hongdou JiaH-Index: 8
Last. Chuang XuH-Index: 8
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During the transition into lactation, bovine mammary epithelial cells (bMECs) are likely subjected to altered redox balance due to the high metabolic rate associated with the onset of lactation. In non-ruminants, lycopene (LYC), a naturally occurring hydrocarbon carotenoid, has attracted considerable attention as a potential natural agent against oxidative stress. The aim of this study was to investigate whether LYC alleviates oxidative injury in bMECs induced by H2O2 and the underlying molecula...
#1Josef Johann Gross (University of Bern)H-Index: 19
: The increasing lactational performance of dairy cows over the last few decades is closely related to higher nutritional requirements. The decrease in dry matter intake during the peripartal period results in a considerable mobilisation of body tissues (mainly fat reserves and muscle mass) to compensate for the prevailing lack of energy and nutrients. Despite the activation of adaptive mechanisms to mobilise nutrients from body tissues for maintenance and milk production, the increased metaboli...
#1Xudong Sun (JLU: Jilin University)H-Index: 8
#2Xiaobing Li (JLU: Jilin University)H-Index: 23
Last. Xinwei Li (JLU: Jilin University)H-Index: 34
view all 13 authors...
ABSTRACT Dairy cows with ketosis display excessive lipolysis in adipose tissue. Heat-shock protein B7 (HSPB7), a small heat-shock protein, plays important roles in mediating cytoprotective responses to oxidative stress in rodent adipose tissue. Accordingly, it is assumed that HSPB7 may also play important roles in the antioxidant response in adipose tissue of ketotic cows. Therefore, the aim of this study is to investigate (1) the redox state of adipose tissue in ketotic cows and (2) the role an...
#1Maria Ruart (University of Barcelona)H-Index: 4
#2Laia Chavarria (University of Barcelona)H-Index: 2
Last. Virginia Hernández-Gea (University of Barcelona)H-Index: 30
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Background & Aims Endothelial dysfunction plays an essential role in liver injury, yet the phenotypic regulation of liver sinusoidal endothelial cells (LSECs) remains unknown. Autophagy is an endogenous protective system whose loss could undermine LSEC integrity and phenotype. The aim of our study was to investigate the role of autophagy in the regulation of endothelial dysfunction and the impact of its manipulation during liver injury. Methods We analyzed primary isolated LSECs from Atg7control...
#4Chen Wei (ZJU: Zhejiang University)H-Index: 28
Abstract Ethyl carbamate (EC) is thought to be a toxicant that widely exists in cigarette smoke and polluted air, as well as fermented food and alcoholic beverages. However, the mechanism and approach to treat hepatic damage after EC exposure remain unclear. Here, we first found that EC caused decreased cell viability, reactive oxygen species (ROS) overproduction and glutathione (GSH) depletion in normal human hepatocytes L02 cells. Excessive ROS generation was found to be one of the major reaso...
#1Dong Li (Nanjing Medical University)H-Index: 2
#2Su Ni (Nanjing Medical University)H-Index: 6
Last. Chao Zhuang (Nanjing Medical University)H-Index: 4
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Chondrocyte apoptosis is closely related to the development and progression of osteoarthritis (OA); however, the underlying mechanisms remain enigmatic. Previous studies have confirmed that cell apoptosis is one of the main pathological alterations during oxidative stress, and chondrocyte apoptosis induced by oxidative stress plays an important role in the development of OA. Rat chondrocytes exposed to hydrogen peroxide (H2O2) were used as the experimental oxidative stress model. We assessed cel...
#1Y.F. MaH-Index: 1
#2Z. H. Wu (Inner Mongolia Agricultural University)H-Index: 2
Last. Juan J. Loor (UIUC: University of Illinois at Urbana–Champaign)H-Index: 71
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ABSTRACT The experiment was conducted to determine the role of nuclear factor (erythroid-derived 2)-like factor 2 (NFE2L2, formerly Nrf2) antioxidant response element (ARE) pathway in protecting bovine mammary epithelial cells (BMEC) against H 2 O 2 -induced oxidative stress injury. An NFE2L2 small interfering RNA (siRNA) interference or a pCMV6-XL5-NFE2L2 plasmid fragment was transfected to independently downregulate or upregulate expression of NFE2L2. Isolated BMEC in triplicate were exposed t...
#1Bowen Wu (CSU: Central South University)H-Index: 3
#2Miduo Tan (CSU: Central South University)H-Index: 1
Last. Xiping Zhang (CSU: Central South University)H-Index: 3
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Abstract Osteosarcoma is a common primary malignant bone tumor, the cure rate of which has stagnated over the past 25–30 years. Autophagy modulation has been considered a potential therapeutic strategy for osteosarcoma, and previous study indicated that arsenic trioxide (ATO) exhibits significant anti-carcinogenic activity. However, the ability of ATO to induce autophagy and its role in osteosarcoma cell death remains unclear. In the present study, we showed that ATO increased autophagic flux in...
#1Daoud Ali (KSU: King Saud University)H-Index: 27
#2Abhilasha TripathiH-Index: 1
Last. Salem Manohardas (KSU: King Saud University)H-Index: 1
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: There are a large number of agricultural workers who are exposed to pesticides through skin and inhalation. The best approach to identify altered molecular pathways during dermal exposure to pesticides is relevant to risk-associated concern about skin safety. In this study, we investigated the cytotoxic effect of zineb, a fungicide, in human keratinocyte (HaCaT) cells. HaCaT cells were treated with zineb (1-40 µg/mL) for 24 hours. Cellular and molecular mechanisms of cell toxicity were investi...
Cited By2
#1Yuanyuan ChenH-Index: 2
#2Yan TangH-Index: 3
Last. Hao Dong
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ABSTRACT null null Bovine mammary epithelial cells undergo an increase in metabolic rate, mitochondrial dysfunction, and oxidative stress after calving. Nuclear factor erythroid 2-related factor 2 (NFE2L2), a master regulator of cellular redox homeostasis, plays crucial roles in the regulation of mitochondrial function. The objective of this study was to investigate the role of NFE2L2 on mitochondrial function in bovine mammary epithelial cells under hyperlipidemic conditions. Three experiments ...
#1John M. BarbaroH-Index: 2
#2Ana Maria CuervoH-Index: 123
Last. Joan W. BermanH-Index: 68
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HIV enters the CNS early after peripheral infection, establishing reservoirs in perivascular macrophages that contribute to development of HIV-associated neurocognitive disorders (HAND) in 15–40% of people with HIV (PWH) despite effective antiretroviral therapy (ART). Opioid use may contribute to dysregulated macrophage functions resulting in more severe neurocognitive symptoms in PWH taking opioids. Macroautophagy helps maintain quality control in long-lived cell types, such as macrophages, and...
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