Cellular benefits of single-use negative pressure wound therapy demonstrated in a novel ex vivo human skin wound model.

Published on Mar 1, 2021in Wound Repair and Regeneration2.471
· DOI :10.1111/WRR.12888
Holly N. Wilkinson8
Estimated H-index: 8
(Hull York Medical School),
Francesca L. Longhorne (Hull York Medical School)+ 2 AuthorsMatthew J. Hardman34
Estimated H-index: 34
(Hull York Medical School)
Negative pressure wound therapy is a widely used treatment for chronic, nonhealing wounds. Surprisingly, few studies have systematically evaluated the cellular and molecular effects of negative pressure treatment on human skin. In addition, no study to date has directly compared recently available single-use negative pressure modalities to traditional negative pressure devices in a controlled setting. Here we developed a novel large-scale ex vivo human skin culture system to effectively evaluate the efficacy of two different negative pressure wound therapy modalities. Single-use and traditional negative pressure devices were applied to human ex vivo wounded skin sheets cultured over a period of 48 hours. Cellular tissue response to therapy was evaluated via a combination of histological analysis and transcriptional profiling, in samples collected from the wound edge, skin adjacent to the wound, and an extended skin region. Single-use negative pressure wound therapy caused less damage to wound edge tissue than traditional application, demonstrated by improved skin barrier, reduced dermal-epidermal junction disruption and a dampened damage response. Transcriptional profiling confirmed significantly less activation of multiple pro-inflammatory markers in wound edge skin treated with single-use vs traditional negative pressure therapy. These findings may help to explain the greater efficacy of sNPWT in the clinic, while offering a noninvasive system to develop improved NPWT-based therapies.
#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Matthew J. Hardman (Hull York Medical School)H-Index: 34
Arguably, the two most important causes of pathological healing in the skin are diabetes and ageing. While these factors have historically been considered independent modifiers of the healing process, recent studies suggest that they may be mechanistically linked. The primary contributor to diabetic pathology is hyperglycaemia, which accelerates the production of advanced glycation end products, a characteristic of ageing tissue. Indeed, advanced age also leads to mild hyperglycaemia. Here, we d...
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#1Varuni R. Brownhill (Smith & Nephew)H-Index: 1
#2Elizabeth Mary Huddleston (Smith & Nephew)H-Index: 6
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#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Matthew J. Hardman (Hull York Medical School)H-Index: 34
Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular beha...
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#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Sophie E. Upson (Hull York Medical School)H-Index: 1
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#1Robert S. Kirsner (UM: University of Miami)H-Index: 77
#2Cyaandi DoveH-Index: 7
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#1Maurício José Lopes Pereima (UFSC: Universidade Federal de Santa Catarina)H-Index: 5
#2Rodrigo FeijóH-Index: 5
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#1Leland Jaffe (RFUMS: Rosalind Franklin University of Medicine and Science)H-Index: 2
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#1Ruth Knight (University of Oxford)H-Index: 5
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#1Lijuan Zhou (ZJU: Zhejiang University)H-Index: 1
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Abstract Human skin organ culture (hSOC) is a simple but highly instructive and clinically relevant skin research method. It has been used for decades to study the development, differentiation, and function as well as the response to wounding or test agents of intact human skin in the presence of its appendages and all resident cell populations. hSOC has also proven useful in toxicological and oncological studies and studies of skin aging (both chronological aging and photoaging), skin energy me...
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