Abstract Background Food-intestine interaction study has always been a hot topic in food science and nutrition due to diverse physiological functions of intestine. Compared to expensive animal models with limited screening capabilities, the simple, reliable and highly reproducible intestinal cell models are widely used in food-intestine interaction study. There are many functional cell models used to simulate the intestine in vitro, among which the Caco-2 cell model is one of the most widely used and classical models. Recently years, the differentiated Caco-2 cell model has been greatly developed due to the development of various technologies, which not only overcomes the limitations of the traditional model, but also further broadens its application. Scope and approach This review aims to overview the current applications of the differentiated Caco-2 cell model as a specialized model of intestinal cells in vitro, as well as new approaches solving the existing challenges of utilization, which can guide its future trends in interaction between food factors and the intestine. Key findings and conclusions: With high flexibility, high repeatability and low cost, the differentiated Caco-2 cell model has been applied to a variety of intestinal studies including intestinal absorption, intestinal transport, intestinal metabolism, intestinal barrier, intestinal immunity and intestinal adhesion. Furthermore, future study should break limitations of traditional models with the help of automation, biochemistry, molecular biology and cells co-culture, so as to make it more closer to the internal environment without sacrificing its simplicity and reliability, and more suitable for cost-effective large-scale analysis of food-intestine interaction.
#2Prabhanshu Tripathi(Translational Health Science and Technology Institute)H-Index: 8
Last. Aparna Dixit(JNU: Jawaharlal Nehru University)H-Index: 17
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Abstract Objectives Hyperglycemia mediated tight junction deformity is known to contribute to leaky gut in various metabolic disorders. The present study aimed to investigate the role of oxidative stress on intestinal epithelial tight junction (TJ) barrier functions in hyperglycemia. Since, many flavonoids are known to influence the cellular redox state, thus exploring these flavonoids may help to understand the role of TJ barrier in hyperglycemia-mediated oxidative stress which might unfolds th...
Abstract Malvidin-3-O-glucoside, malvidin-3,5-O-diglucoside, malvidin-3-O-(6-O-coumaroyl)-glucoside-5-O-glucoside from Chinese Vitis davidii red wine were used to investigate the role of glucoside, diglucoside and coumaroylated glucoside moieties on their transport efficiency through MKN-28 gastric and Caco-2 intestinal cells. Due to the already described conversion of 3-O-glucosilated anthocyanins into 3-O-glucuronilated, the 3-O-glucuronilated metabolite of malvidin-3-O-glucoside was also test...
#2Jessica Ison(University of Louisville)H-Index: 1
Last. Neetu Tyagi(University of Louisville)H-Index: 52
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Abstract The gut microbiota (GM) is referred to as the second gene pool of the human body and a commensal, symbiotic, and pathogenic microorganism living in our intestines. The knowledge of the complex interaction between intestinal microbiota and health outcomes is a novel and rapidly expanding the field. Earlier studies have reported that the microbial communities affect the cellular responses and shape many aspects of physiology and pathophysiology within the body, including muscle and bone m...
Last. Mingyong Xie(NCU: Nanchang University)H-Index: 71
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Abstract Lipopolysaccharide (LPS)-induced inflammatory macrophages model and intestinal-like Caco-2/macrophages co-culture inflammation model were established to evaluate the anti-inflammatory effect and underlying mechanism of Ganoderma atrum polysaccharides (PSG-1). It was found that PSG-1 reduced LPS-induced secretion of pro-inflammatory cytokine (TNF-α, IL-6 and IL-1β), ROS levels, and inhibited the expression of COX-2 in LPS-stimulated inflammatory macrophages model and intestinal-like Caco...
Abstract We have previously demonstrated that human vaginal Lactobacillus crispatus 2029 (LC2029) strain is highly adhesive to cervicovaginal epithelial cells, exhibits antagonistic activity against genitourinary pathogens and expresses surface-layer protein (Slp). The aims of the present study were elucidation of Slp structural and immunomodulatory characteristics and its roles in protective properties of the whole vaginal LC2029 bacteria against foodborne pathogens. Enteric Caco-2 and colon HT...
Last. Magali Genay(University of Lorraine)H-Index: 9
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Abstract The mucus, mainly composed of the glycoproteins mucins, is a rheological substance that covers the intestinal epithelium and acts as a protective barrier against a variety of harmful molecules, microbial infection and varying lumen environment conditions. Alterations in the composition or structure of the mucus could lead to various diseases such as inflammatory bowel disease or colorectal cancer. Recent studies revealed that an exogenous intake of probiotic bacteria or other dietary co...
Last. Mingyong Xie(Jiangxi Normal University)H-Index: 71
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Abstract Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) activated the immune response of dendritic cells (DCs) by direct contacting with surface receptors. However, as a kind of macromolecule compound, PSG-1 cannot directly stimulate DCs via passing through the intestine epithelial cells. To investigate the role of PSG-1 in the interaction between the intestinal epithelium and its internal immune cells, the intestinal-like Caco-2/DCs co-culture model was establis...
#1Ume Roobab(H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 6
#2Zahra Batool(SCUT: South China University of Technology)H-Index: 1
Last. Rana Muhammad Aadil(H.I., S.I.: University of Agriculture, Faisalabad)H-Index: 19
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Probiotics are living organisms and pose diverse applications to modify food constituents for health benefits of humans. There is an increasing trend to get insights into developments of new technologies to improve their formulations for better use as a food product. Moreover, the new and beneficial aspects of probiotics have made them as a best replacement of antibiotics to combat even more complex disorders, such as gastrointestinal (GI), nervous system and digestive system. The aim of present...
#1Mohd Iqbal Bhat(NDRI: National Dairy Research Institute)H-Index: 6
#2Suman Kapila(NDRI: National Dairy Research Institute)H-Index: 22
Last. Rajeev Kapila(NDRI: National Dairy Research Institute)H-Index: 21
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Abstract Dietary intervention using probiotic bacteria has emerged a promising therapeutic strategy to curb gastrointestinal diseases. To test this hypothesis, present investigation was aimed to assess in vitro prophylactic capability of probiotic Lactobacillus fermentum (LF: MTCC-5898) against Escherichia coli (ATCC 14948) impaired barrier function using human intestinal Caco-2 cells. L. fermentum adhered strongly with Caco-2 cells (1850 ± 104.1 CFU/100 cells) and maintained host barrier integr...
Abstract The presence of commensal fungal species in the human gut indicates that organisms from this kingdom have the potential to benefit the host as well. Saccharomyces boulardii, a yeast strain isolated about a hundred years ago, is the most well-characterized probiotic yeast. Though for the most part it genetically resembles Saccharomyces cerevisiae, specific phenotypic differences make it better suited for the gut microenvironment such as better acid and heat tolerance. Several studies usi...
Abstract null null Background null Foods are natural sources of antioxidant compounds, that are known for their role in preventing many human diseases. Although several methods to assess the antioxidant activity/capacity of foods and their bioactive components have been developed, a simple universal method has not been proposed. Since both in vitro and in vivo assays have limitations, cell-based assays are gaining plenty of attention as a more suitable middle ground between in vitro chemical ass...
The Caco-2 human intestinal cell line has been widely adopted to mimic the in vivo small intestinal epithelium for screening the characteristics and mechanisms of the uptake, metabolism and absorption of dietary compounds and drugs. Marked advances in stem cell biology and tissue engineering have led to the development of novel organoid and microfluidic models that better replicate key aspects of the physiology of the human gut and are expected to generate more biologically relevant information ...
#1Feng Xue(Nanjing University of Chinese Medicine)H-Index: 9
#2Xuan Li(Nanjing University of Chinese Medicine)H-Index: 1
Last. Benu Adhikari(RMIT: RMIT University)H-Index: 63
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Aging is spontaneous and inevitable process in all living beings. It is a complex natural phenomenon that manifests as a gradual decline of physiological functions and homeostasis. Aging inevitably leads to age-associated injuries, diseases, and eventually death. The research on aging-associated diseases aimed at delaying, preventing or even reversing the aging process are of great significance for healthy aging and also for scientific progress. Numerous plant-derived compounds have anti-aging e...
#1Ruihong Yu(Fujian Agriculture and Forestry University)H-Index: 2
#2Mengke Sun(Fujian Agriculture and Forestry University)
Last. Zhe Ren(Fujian Agriculture and Forestry University)H-Index: 6
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The aim of this study was to explore the indirect immunomodulatory activities and its mechanism of enzymatic hydrolysis of Hericium erinaceus polysaccharides (EHEP) in the MODE-K/DCs co-culture model. According to the TEER value, transmission of phenol red and AKP activity of MODE-K cells, single model was established in order to evaluate the eligibility of MODE-K cells monolayer. Then the MODE-K/DCs co-culture model was set up and HEP and EHEP were added into the apical chamber, DCs were obtain...
Last. Qiang Yu(NCU: Nanchang University)H-Index: 21
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Abstract The previous research has indicated that Ganoderma atrum polysaccharide (PSG-1) indirectly affects the immune function of dendritic cells (DCs) in intestinal-like Caco-2/DCs co-culture model, in which NF-κB and MAPK pathway play an essential role. To explore the interaction of Caco-2 in the interaction between the intestinal epithelium and its internal immune cells, the intestinal-like Caco-2/DCs co-culture model was developed. All transcripts of Caco-2 treated with or without PSG-1 wer...