Pterostilbene inhibits deoxynivalenol-induced oxidative stress and inflammatory response in bovine mammary epithelial cells.

Published on Jan 15, 2021in Toxicon2.201
· DOI :10.1016/J.TOXICON.2020.11.002
Jing Zhang5
Estimated H-index: 5
(JLU: Jilin University),
Junmei Wang4
Estimated H-index: 4
(Sichuan Agricultural University)
+ 5 AuthorsYongcheng Jin8
Estimated H-index: 8
(JLU: Jilin University)
Sources
Abstract
Abstract More and more studies have showed that tricothecene mycotoxin, deoxynivalenol (DON) caused cytotoxicity in mammary alveolar cells-large T antigen cells (MAC-T). Therefore, research on reducing the cytotoxicity of DON has gradually attracted attention. In this study, we aim to explore the potential of pterostilbene (PTE) to protect MAC-T cells from DON-induced oxidative stress and inflammatory response. MAC-T cells were treated with 0.25 μg/mL DON or 2.0504 μg/mL PTE or 0.25 μg/mL DON and 2.0504 μg/mL PTE together, incubated for 9 h. PTE effectively improved cell viability, cell proliferation and total antioxidant capacity (T-AOC), reduced reactive oxygen species (ROS) production and malondialdehyde (MDA), and improved glutathione (GSH) depletion. Moreover, PTE effectively regulated the mRNA levels of nuclear factor erythroid-2-related factor 2 (Nrf2), kelch-like ech-associated protein 1 (Keap1), superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2). PTE significantly inhibited nuclear factor kappa-B P65 (NF-κB P65), nuclear factor kappa-B P50 (NF-κB P50), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and monocyte chemotactic protein 1 (MCP-1) mRNA levels in DON-induced MAC-T cells. PTE also significantly reduced inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in DON-induced MAC-T cells. Additionally, ELISA revealed that PTE inhibited the expression of tumor necrosis factor-α (TNF-α) and IL-6 proteins produced in DON-induced MAC-T cells. These findings together provided strong evidence to support that PTE can effectively alleviate the damage to cells caused by DON, and it may be used as an effective anti-inflammatory and antioxidant to prevent the damage of mycotoxins to the animal body.
References67
Newest
#1Hao Zhang (NAU: Nanjing Agricultural University)H-Index: 18
#2Yanan Chen (NAU: Nanjing Agricultural University)H-Index: 5
Last. Tian Wang (NAU: Nanjing Agricultural University)H-Index: 25
view all 8 authors...
Abstract Objectives The aim of the present study was to investigate the potential of pterostilbene, a beneficial component primarily found in blueberries, to alleviate the intra-uterine growth retardation (IUGR)-induced early liver injury and oxidative stress in a porcine model. Research Methods & Procedures Thirty-six IUGR piglets and an equal number of normal birth weight (NBW) counterparts received a diet with or without pterostilbene (250 mg/kg diet) during the first week post-weaning. Param...
2 CitationsSource
#1Rym BarboucheH-Index: 14
#2Stéphanie GaigéH-Index: 9
Last. Anne AbysiqueH-Index: 11
view all 7 authors...
The ribotoxin deoxynivalenol (DON) is a trichothecene found on cereals responsible for mycotoxicosis in both humans and farm animals. DON toxicity is characterized by reduced food intake, diminished nutritional efficiency and immunologic effects. The present study was designed to further characterize the alterations in energy metabolism induced by DON intoxication. We demonstrated that acute DON intoxication triggered liver steatosis associated with an altered expression of genes related to lipi...
1 CitationsSource
#1Eva Vaňková (Institute of Chemical Technology in Prague)H-Index: 5
#2Martina Paldrychová (Institute of Chemical Technology in Prague)H-Index: 3
Last. Jan Masák (Institute of Chemical Technology in Prague)H-Index: 17
view all 8 authors...
Pterostilbene (PTE), a dimethylated analogue of resveratrol, mostly contained in Vitis vinifera leaves or in other plant sources is well-known for its antioxidant activity. Due to its bioavailability, low hydrophilicity and thus ability to penetrate hydrophobic biological membranes it was found to be an antimicrobial agent. These properties of PTE offer the possibility of its use in the treatment of microbial infections. The emergence of antibiotic resistance of microorganisms is often caused by...
2 CitationsSource
#1Huahong Yang (JLU: Jilin University)H-Index: 5
#2Cong Hua (JLU: Jilin University)H-Index: 1
Last. Xinxin Ci (JLU: Jilin University)H-Index: 22
view all 7 authors...
Early pulmonary fibrosis after acute lung injury leads to poor prognosis and high mortality. Pterostilbene (Pts), a bioactive component in blueberries, possesses anti-inflammatory, antioxidative and antifibrotic properties. However, the effects of Pts on lipopolysaccharide (LPS) -induced pulmonary fibrosis are still unknown. In our study, the therapeutic effect of Pts treatment in mice led to lower lung injury and fibrosis scores, and lower levels of hydroxyproline and protein (collagen I and TG...
7 CitationsSource
#1Pablo Peñalver (CSIC: Spanish National Research Council)H-Index: 11
#2Sonia Zodio (CSIC: Spanish National Research Council)H-Index: 1
Last. Juan Carlos Morales (CSIC: Spanish National Research Council)H-Index: 52
view all 5 authors...
Oxidative stress is known to be a key factor in many neurodegenerative diseases. Inflammation also plays a relevant role in a myriad of pathologies such as diabetes and atherosclerosis. Polyphenols coming from dietary sources, such as pterostilbene, may be beneficial in this type of diseases. However, most of them are rapidly metabolized and excreted yielding very low phenolic bioavailability what makes difficult to find out which are the mechanisms responsible for the observed bioactivity. Here...
9 CitationsSource
#1Sakshi Mishra (Central Drug Research Institute)H-Index: 9
#2Sonal Srivastava (Central Drug Research Institute)H-Index: 7
Last. Srikanta Kumar Rath (Central Drug Research Institute)H-Index: 13
view all 5 authors...
AbstractPreventing food from fungal infestation has become a cause of great concern as food safety is of particular importance to public health globally. Recently International Agency for Research ...
30 CitationsSource
#1Mari Eskola (BOKU: University of Natural Resources and Life Sciences, Vienna)H-Index: 11
#2Gregor Kos (Concordia University)H-Index: 16
Last. Rudolf Krska (BOKU: University of Natural Resources and Life Sciences, Vienna)H-Index: 84
view all 6 authors...
AbstractPrior to 1985 the Food and Agriculture Organization (FAO) estimated global food crop contamination with mycotoxins to be 25%. The origin of this statement is largely unknown. To assess the ...
118 CitationsSource
#1Xiaolian Gu (Wuhan Polytechnic University)H-Index: 2
#2Wenyan Guo (Wuhan Polytechnic University)H-Index: 2
Last. Chao Chang (Wuhan Polytechnic University)H-Index: 2
view all 6 authors...
With the purpose to explore the relationship between deoxynivalenol (DON)-induced apoptosis and autophagy and provide mechanistic explanations for the toxic effects of DON on IPEC-J2 cells, we determined the cell viability, cell morphology, apoptosis, and autophagy by using autophagy inhibitor 3-methyladenine (3-MA), PI3K pathway inhibitor LY294002, and activator 740Y-P. It turned out that 3-MA was able to attenuate the reduction of cell viability induced by DON. Moreover, 3-MA was capable of up...
1 CitationsSource
#1Junmei Wang (JLU: Jilin University)H-Index: 4
#2Yongcheng Jin (JLU: Jilin University)H-Index: 8
Last. Jing Zhang (JLU: Jilin University)H-Index: 5
view all 16 authors...
Deoxynivalenol (DON) is a toxic secondary metabolite produced by Fusarium graminearum. It is one of the most common feed contaminants that poses a serious threat to the health and performance of dairy cows. This study investigated the in vitro cytotoxicity of DON on bovine mammary epithelial cells (MAC-T). DON at different concentrations (0.25, 0.3, 0.5, 0.8, 1 or 2 μg/ml) inhibited the growth of MAC-T cells after 24 hr of exposure (p < .001). DON at 0.25 μg/ml increased lactate dehydrogenase (L...
7 CitationsSource
#1Ruifen Kang (NAU: Nanjing Agricultural University)H-Index: 1
#2Ruonan Li (NAU: Nanjing Agricultural University)H-Index: 3
Last. Chunmei Li (NAU: Nanjing Agricultural University)H-Index: 23
view all 6 authors...
Abstract Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. To investigate the underlying mechanism of DON-induced apoptosis and inflammation in porcine small intestinal epithelium, intestinal porcine epithelial cells (IPEC-J2 cells) were chosen as objects, and were treated by different concentrations (0 μg/mL, 0.2 μg/mL, 0.5 μg/mL, 1.0 μg/mL, 2.0 μg/mL, 4.0 μg/mL, 6.0 μg/mL) of DON. The results showed that DON induced cytoto...
34 CitationsSource
Cited By1
Newest
Source
#1Ramanathan K. Kasimanickam (WSU: Washington State University)H-Index: 23
#2Vanmathy R. Kasimanickam (WSU: Washington State University)H-Index: 14
Abstract The objective was to elucidate the relationships among gastrointestinal (GI) parasite load, serum cytokines (Th 1 – Interleukin (IL) 2, Interferon (IFN) γ and Tumor necrosis factor (TNF) α; Th 2- IL4, IL6, and IL10) levels, and hormones (progesterone, cortisol, 8-epi-prostaglandin F2 alpha (isoprostane), prolactin, substance-p, and prostaglandin F metabolites) concentrations, and pregnancy in beef cattle. Angus-cross beef cows (n = 700; age, 3 to 8 y) were blocked by age and body condit...
Source
#1A. Mavrommatis (AUA: Agricultural University of Athens)H-Index: 7
#2Elisavet Giamouri (AUA: Agricultural University of Athens)H-Index: 1
Last. K Feggeros (AUA: Agricultural University of Athens)H-Index: 3
view all 11 authors...
Mycotoxins appear to be the “Achilles’ heel” of the agriculture sector inducing enormous economic losses and representing a severe risk to the health of humans and animals. Although novel determination protocols have been developed and legislation has been implemented within Europe, the side effects of mycotoxins on the homeostatic mechanisms of the animals have not been extensively considered. Feed mycotoxin contamination and the effects on the antioxidant status of livestock (poultry, swine, a...
Source