Pterostilbene inhibits deoxynivalenol-induced oxidative stress and inflammatory response in bovine mammary epithelial cells.

Published on Jan 15, 2021in Toxicon2.201
· DOI :10.1016/J.TOXICON.2020.11.002
Jing Zhang5
Estimated H-index: 5
(JLU: Jilin University),
Junmei Wang4
Estimated H-index: 4
(Sichuan Agricultural University)
+ 5 AuthorsYongcheng Jin8
Estimated H-index: 8
(JLU: Jilin University)
Abstract More and more studies have showed that tricothecene mycotoxin, deoxynivalenol (DON) caused cytotoxicity in mammary alveolar cells-large T antigen cells (MAC-T). Therefore, research on reducing the cytotoxicity of DON has gradually attracted attention. In this study, we aim to explore the potential of pterostilbene (PTE) to protect MAC-T cells from DON-induced oxidative stress and inflammatory response. MAC-T cells were treated with 0.25 μg/mL DON or 2.0504 μg/mL PTE or 0.25 μg/mL DON and 2.0504 μg/mL PTE together, incubated for 9 h. PTE effectively improved cell viability, cell proliferation and total antioxidant capacity (T-AOC), reduced reactive oxygen species (ROS) production and malondialdehyde (MDA), and improved glutathione (GSH) depletion. Moreover, PTE effectively regulated the mRNA levels of nuclear factor erythroid-2-related factor 2 (Nrf2), kelch-like ech-associated protein 1 (Keap1), superoxide dismutase 1 (SOD1) and superoxide dismutase 2 (SOD2). PTE significantly inhibited nuclear factor kappa-B P65 (NF-κB P65), nuclear factor kappa-B P50 (NF-κB P50), cyclooxygenase-2 (COX-2), interleukin-1β (IL-1β), interleukin-6 (IL-6) and monocyte chemotactic protein 1 (MCP-1) mRNA levels in DON-induced MAC-T cells. PTE also significantly reduced inducible nitric oxide synthase (iNOS) and nitric oxide (NO) levels in DON-induced MAC-T cells. Additionally, ELISA revealed that PTE inhibited the expression of tumor necrosis factor-α (TNF-α) and IL-6 proteins produced in DON-induced MAC-T cells. These findings together provided strong evidence to support that PTE can effectively alleviate the damage to cells caused by DON, and it may be used as an effective anti-inflammatory and antioxidant to prevent the damage of mycotoxins to the animal body.
#1Hao Zhang (NAU: Nanjing Agricultural University)H-Index: 18
#2Yanan Chen (NAU: Nanjing Agricultural University)H-Index: 5
Last. Tian Wang (NAU: Nanjing Agricultural University)H-Index: 25
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Abstract Objectives The aim of the present study was to investigate the potential of pterostilbene, a beneficial component primarily found in blueberries, to alleviate the intra-uterine growth retardation (IUGR)-induced early liver injury and oxidative stress in a porcine model. Research Methods & Procedures Thirty-six IUGR piglets and an equal number of normal birth weight (NBW) counterparts received a diet with or without pterostilbene (250 mg/kg diet) during the first week post-weaning. Param...
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#1Rym BarboucheH-Index: 14
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With the purpose to explore the relationship between deoxynivalenol (DON)-induced apoptosis and autophagy and provide mechanistic explanations for the toxic effects of DON on IPEC-J2 cells, we determined the cell viability, cell morphology, apoptosis, and autophagy by using autophagy inhibitor 3-methyladenine (3-MA), PI3K pathway inhibitor LY294002, and activator 740Y-P. It turned out that 3-MA was able to attenuate the reduction of cell viability induced by DON. Moreover, 3-MA was capable of up...
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Deoxynivalenol (DON) is a toxic secondary metabolite produced by Fusarium graminearum. It is one of the most common feed contaminants that poses a serious threat to the health and performance of dairy cows. This study investigated the in vitro cytotoxicity of DON on bovine mammary epithelial cells (MAC-T). DON at different concentrations (0.25, 0.3, 0.5, 0.8, 1 or 2 μg/ml) inhibited the growth of MAC-T cells after 24 hr of exposure (p < .001). DON at 0.25 μg/ml increased lactate dehydrogenase (L...
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Abstract Deoxynivalenol (DON) frequently detected in a wide range of foods and feeds, inducing cytotoxicity to animals and humans. To investigate the underlying mechanism of DON-induced apoptosis and inflammation in porcine small intestinal epithelium, intestinal porcine epithelial cells (IPEC-J2 cells) were chosen as objects, and were treated by different concentrations (0 μg/mL, 0.2 μg/mL, 0.5 μg/mL, 1.0 μg/mL, 2.0 μg/mL, 4.0 μg/mL, 6.0 μg/mL) of DON. The results showed that DON induced cytoto...
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