Fusion peptide engineered "statically-versatile" titanium implant simultaneously enhancing anti-infection, vascularization and osseointegration.

Published on Jan 1, 2021in Biomaterials10.317
· DOI :10.1016/J.BIOMATERIALS.2020.120446
Junjian Chen11
Estimated H-index: 11
(SCUT: South China University of Technology),
Guansong Hu3
Estimated H-index: 3
(SCUT: South China University of Technology)
+ 7 AuthorsYingjun Wang42
Estimated H-index: 42
(SCUT: South China University of Technology)
Abstract Although antimicrobial titanium implants can prevent biomaterial-associated infection (BAI) in orthopedics, they display cytotoxicity and delayed osseointegration. Therefore, versatile implants are desirable for simultaneously inhibiting BAI and promoting osseointegration, especially “statically-versatile” ones with nonessential external stimulations for facilitating applications. Herein, we develop a “statically-versatile” titanium implant by immobilizing an innovative fusion peptide (FP) containing HHC36 antimicrobial sequence and QK angiogenic sequence via sodium borohydride reduction promoted Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC-SB), which shows higher immobilization efficiency than traditional CuAAC with sodium ascorbate reduction (CuAAC-SA). The FP-engineered implant exhibits over 96.8% antimicrobial activity against four types of clinical bacteria (S. aureus, E. coli, P. aeruginosa and methicillin-resistant S. aureus), being stronger than that modified with mixed peptides. This can be mechanistically attributed to the larger bacterial accessible surface area of HHC36 sequence. Notably, the implant can simultaneously enhance cellular proliferation, up-regulate expressions of angiogenesis-related genes/proteins (VEGF and VEGFR-2) of HUVECs and osteogenesis-related genes/proteins (ALP, COL-1, RUNX-2, OPN and OCN) of hBMSCs. In vivo assay with infection and non-infection bone-defect model reveals that the FP-engineered implant can kill 99.63% of S. aureus, and simultaneously promote vascularization and osseointegration. It is believed that this study presents an excellent strategy for developing “statically-versatile” orthopedic implants.
#1Nastaran Abbasizadeh (UT: University of Tehran)H-Index: 1
#2Ali Hossein Rezayan (UT: University of Tehran)H-Index: 24
Last. Mehdi Kazemzadeh-Narbat (Brigham and Women's Hospital)H-Index: 13
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AbstractImproving osseointegration together with preventing infections are the main challenges of using titanium-based implants in joint replacement surgeries. In this study, a hybrid composite of silk fibroin (SF)/hydroxyapatite (HA) nanofibrous mats was directly electrospun on titanium plates in order to mimic the bone extracellular structure and improve osteoconductivity. Afterwards, HHC-36 antimicrobial peptide (AMP) was immobilized on the fabricated mats using dopamine as a linker. The morp...
10 CitationsSource
#1Junjian Chen (SCUT: South China University of Technology)H-Index: 11
#2Xuetao Shi (SCUT: South China University of Technology)H-Index: 36
Last. Yingjun Wang (SCUT: South China University of Technology)H-Index: 42
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#1Lin Wang (SCUT: South China University of Technology)H-Index: 20
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Cited By2
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