Immunization with the receptor-binding domain of SARS-CoV-2 elicits antibodies cross-neutralizing SARS-CoV-2 and SARS-CoV without antibody-dependent enhancement

Published on Sep 3, 2020in Cell discovery6.255
· DOI :10.1038/S41421-020-00199-1
Jinkai Zang3
Estimated H-index: 3
(CAS: Chinese Academy of Sciences),
Chenjian Gu10
Estimated H-index: 10
(Fudan University)
+ 12 AuthorsZhong Huang29
Estimated H-index: 29
(CAS: Chinese Academy of Sciences)
Zhou, P. et al. A pneumonia outbreak associated with a new coronavirus of probable bat origin. Nature 579, 270–273 (2020). CAS Article Google Scholar Amanat, F. & Krammer, F. SARS-CoV-2 vaccines: status report. Immunity 52, 583–589 (2020). CAS Article Google Scholar Chen, W. H., Strych, U., Hotez, P. J. & Bottazzi, M. E. The SARS-CoV-2 vaccine pipeline: an overview. Curr. Trop. Med. Rep. 7, 61–64 (2020). Article Google Scholar Hotez, P. J., Corry, D. B. & Bottazzi, M. E. COVID-19 vaccine design: the Janus face of immune enhancement. Nat. Rev. Immunol. 20, 347–348 (2020). CAS Article Google Scholar Huisman, W., Martina, B. E. E., Rimmelzwaan, G. F., Gruters, R. A. & Osterhaus, A. D. M. E. Vaccine-induced enhancement of viral infections. Vaccine 27, 505–512 (2009). CAS Article Google Scholar Zhou, Y. S., Jiang, S. B. & Du, L. Y. Prospects for a MERS-CoV spike vaccine. Expert Rev. Vaccines 17, 677–686 (2018). CAS Article Google Scholar Jiang, S. B., Lu, L., Liu, Q., Xu, W. & Du, L. Y. Receptor-binding domains of spike proteins of emerging or re-emerging viruses as targets for development of antiviral vaccines. Emerg. Microbes Infect. 1, 1–8, e13 (2012) . Article Google Scholar Wang, C. et al. A human monoclonal antibody blocking SARS-CoV-2 infection. Nat. Commun. 11, 2251 (2020). CAS Article Google Scholar Block, O. K. et al. A tetravalent recombinant dengue domain III protein vaccine stimulates neutralizing and enhancing antibodies in mice. Vaccine 28, 8085–8094 (2010). CAS Article Google Scholar Sun, J., Li, M., Wang, Y., Hao, P. & Jin, X. Elaboration of tetravalent antibody responses against dengue viruses using a subunit vaccine comprised of a single consensus dengue envelope sequence. Vaccine 35, 6308–6320 (2017). CAS Article Google Scholar Download references We thank Dr. Xiaozhen Liang for providing A20 and K562 cell lines, Dr. Guangxun Meng for THP-1 cell line, Drs. Gary Wong and Jiaming Lan for codon-optimized S gene, Dr. Haikun Wang for assistance in flow cytometry analysis, and Xueyang Zhang, Yu Zhou, Yang Wu and Zhiping Sun for technical assistance. This study was supported by grants from the Chinese Academy of Sciences (XDB29040300) and from the Chinese Ministry of Science and Technology (2020YFC0845900). The BSL-3 lab of Fudan University was supported by Shanghai Science and Technology Committee and Project of Novel Coronavirus Research from Fudan University. These authors contributed equally: Jinkai Zang, Chenjian Gu, Bingjie Zhou, Chao Zhang, Yong Yang. CAS Key Laboratory of Molecular Virology & Immunology, Institut Pasteur of Shanghai, Center for Biosafety Mega-Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai 200031, China Jinkai Zang, Bingjie Zhou, Chao Zhang, Yong Yang, Shiqi Xu, Lulu Bai, Hong Tang, Dimitri Lavillette & Zhong Huang Key Laboratory of Medical Molecular Virology (MOE/NHC/CAMS), Department of Medical Microbiology and Parasitology, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200031, China Chenjian Gu, Rong Zhang, Qiang Deng, Zhenghong Yuan & Youhua Xie BSL-3 Laboratory of Fudan University, School of Basic Medical Sciences, Shanghai Medical College, Fudan University, Shanghai 200032, China Di Qu You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar You can also search for this author in PubMed Google Scholar Z.H., Y.X., and D.L. conceived and designed the experiments. J.Z., C.G., B.Z., C.Z., Y.Y., S.X., L.B., R.Z., Q.D., Z.Y., H.T., and D.Q. participated in multiple experiments; Z.H., Y.X., D.L., J.Z., C.G., B.Z., C.Z., and Y.Y. analyzed the data. Z.H., Y.X., D.L., and C.Z. wrote the manuscript. Z.H., Y.X., and D.L. provided the final approval of the paper. Correspondence to Dimitri Lavillette or Youhua Xie or Zhong Huang. The authors declare that they have no conflict of interest. Publisher’s note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit Reprints and Permissions Zang, J., Gu, C., Zhou, B. et al. Immunization with the receptor-binding domain of SARS-CoV-2 elicits antibodies cross-neutralizing SARS-CoV-2 and SARS-CoV without antibody-dependent enhancement. Cell Discov 6, 61 (2020)....
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