Osteoarthritis (OA) is a disease that leads to the damage of subchondral bone. To treat OA, patients can have surgery to implant biomaterials into the damaged area. In this research, biomaterials of 3D porous scaffolds were fabricated by the use of air microbubbles for subchondral bone formation proposed for OA surgery. Microbubbles were generated in a polyvinyl alcohol solution at various air flow rates of 20 (F20), 100 (F100), 200 (F200), and 300 (F300) cc min-1. Molecular organization, structure, and morphology of the scaffolds were characterized and observed by Fourier transform infrared spectroscopy, a differential scanning calorimeter, and a scanning electron microscope, respectively. Physical and mechanical properties based on swelling behavior and compressive strength of the scaffolds were also evaluated. Biological performance by means of osteoblast proliferation, protein synthesis, and alkaline phosphatase activity of the scaffolds were studied. The scaffolds showed molecular organization via interaction of -OH and C = O. They had residual water in their structures. The scaffolds exhibited a morphology of a spherical-like cell shape with small pores and a rough surface produced on each cell. Each cell was well connected with the others. The cell size and porous structure of the scaffolds depended significantly on the flow rate used. The molecular organization, structure, and morphology of the scaffolds had an effect on their physical and mechanical properties and biological performance. F100 was found to be an optimum scaffold offering a molecular organization, structure, morphology, physical and mechanical properties, and biological performance which was suitable for subchondral bone formation. This research deduced that the F100 scaffold is promising for OA surgery.
Abstract Natural rubber latex foams (NRLFs) are manufactured by Dunlop and Talalay processes. Although, Talalay process offers the foams with better flexibility and physical properties than the Dunlop does, Dunlop process is mainly used in Thailand because of low production cost. In this study, NRLFs with spherical cell-shape and exceptional interconnective foam network were simply fabricated using an alternative process called Bubbling technique, instead of the complicated and expensive Talalay...
Last. Jirut Meesane(PSU: Prince of Songkla University)H-Index: 8
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Abstract Osteoarthritis (OA) is a degenerative joint disease of the articular cartilage and extends to the subchondral bone. The interface between these soft and hard tissues has a significant role in OA. In this work, gelatin/PVA hydrogels in different ratios of 100:0, 70:30, 50:50, 30:70, and 0:100 were prepared by chemical and freeze-thaw physical crosslinking. The molecular organization and morphology were characterized and observed by FTIR and SEM, respectively. The physical properties of s...
: Tissue engineering embraces the potential of recreating and replacing defective body parts by advancements in the medical field. Being a biocompatible nanomaterial with outstanding physical, chemical, optical, and biological properties, graphene-based materials were successfully employed in creating the perfect scaffold for a range of organs, starting from the skin through to the brain. Investigations on 2D and 3D tissue culture scaffolds incorporated with graphene or its derivatives have reve...
Abstract This study presents production and biomimetic characterization of macroporous composite scaffolds based on gellan gum and nanoparticulate bioactive glass (GG-BAG) under conditions relevant for bone tissue engineering. Formation of hydroxyapatite (HAp) within the scaffolds was investigated in the simulated body fluid (SBF) during 14 days in two biomimetic bioreactors: perfusion bioreactor (1.1 ml/min SBF flowrate) and a bioreactor with coupled dynamic compression and SBF perfusion (5% st...
#1Bhasha Sharma(NSIT: Netaji Subhas Institute of Technology)H-Index: 7
#2Sanjeev Gautam(NSIT: Netaji Subhas Institute of Technology)H-Index: 27
Last. Purnima Jain(NSIT: Netaji Subhas Institute of Technology)H-Index: 8
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Abstract The integration of chemically functionalized RGO into PVA matrix via conventional biomimetic technique to enhance its electrochemical performance. Chemical reduction of exfoliated GO by employing hydrazine hydrate has been conducted which showed uniformity in interfacial dispersion. The modulation readily enhances electrical conductivity of the nanocomposite & can be effectively used in energy storage devices. It is elucidated that transformation of RGO sheets by acquisition of oxygen &...
The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular ...
Background: Here, electrospun fibers based on a blend of polycaprolactone (PCL), poly(ethylene glycol) (PEG), and gelatin methacryloyl (GelMA) were developed. The careful choice of this polymer combination allowed for the preparation of a biomaterial that preserved the mechanical strength of PCL, while at the same time improving the hydrophilicity of the blended material and human osteoblast maturation. Methods: The morphology, chemical structure, wettability, and mechanical properties before an...
Objectives The ability to determine human bone stiffness is of clinical relevance in many fields, including bone quality assessment and orthopaedic prosthesis design. Stiffness can be measured usin...
Subchondral bone plays a critical role in the pathogenesis of osteochondral disease across veterinary species. The subchondral bone is highly adaptable, with the ability to model and remodel in response to loading stresses experienced by the joint. Repetitive stress injuries within the joint can result in primary or secondary pathologic lesions within the subchondral bone, which have been recognized to contribute to the development and progression of osteoarthritis. Recent advances in diagnostic...
In recent years, bone tissue engineering has emerged as a promising solution to the limitations of current gold standard treatment options for bone related-disorders such as bone grafts. Bone tissue engineering provides a scaffold design that mimics the extracellular matrix, providing an architecture that guides the natural bone regeneration process. During this period, a new generation of bone tissue engineering scaffolds has been designed and characterized that explores the incorporation of si...