Evidence of potent humoral immune activity in COVID-19-infected kidney transplant recipients.

Published on Aug 12, 2020in American Journal of Transplantation7.338
· DOI :10.1111/AJT.16261
Susan Hartzell4
Estimated H-index: 4
(ISMMS: Icahn School of Medicine at Mount Sinai),
Sofia Bin2
Estimated H-index: 2
(ISMMS: Icahn School of Medicine at Mount Sinai)
+ 10 AuthorsPaolo Cravedi35
Estimated H-index: 35
(ISMMS: Icahn School of Medicine at Mount Sinai)
Whether kidney transplant recipients are capable of mounting an effective anti-SARS-CoV-2 adaptive immune response despite chronic immunosuppression is unknown and has important implications for therapy. Herein, we analyzed peripheral blood cell surface and intracellular cytokine phenotyping by flow cytometry along with serum antibody testing in 18 kidney transplant recipients with active COVID-19 infection and 36 matched, transplanted controls without COVID-19. We observed significantly fewer total lymphocytes, fewer circulating memory CD4+ and CD8+ T cells in the COVID-19 subjects. We also showed fewer anergic and senescent CD8+ T cells in COVID-19 individuals, but no differences in exhausted CD8+ T cells, nor in any of these CD4+ T cell subsets between groups. We also observed greater frequencies of activated B cells in the COVID-19 patients. Sixteen of 18 COVID-19 subjects tested for anti-SARS-CoV-2 serum antibodies showed positive IgM or IgG titers. Additional analyses showed no significant correlation s among immune phenotypes and degrees of COVID-19 disease severity. Our findings indicate that immunosuppressed kidney transplant recipients admitted to the hospital with acute COVID-19 infection can mount SARS-CoV-2-reactive adaptive immune responses. The findings raise the possibility that empiric reductions in immunosuppressive therapy for all kidney transplant recipients with active COVID-19 may not be required.
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