Cajanolactone A, a stilbenoid from cajanus cajan, prevents ovariectomy-induced obesity and liver steatosis in mice fed a regular diet.

Published on Jul 24, 2020in Phytomedicine4.268
· DOI :10.1016/J.PHYMED.2020.153290
Zhuo-Hui Luo2
Estimated H-index: 2
(Guangzhou University of Chinese Medicine),
Zhi-Wen Liu (Guangzhou University of Chinese Medicine)+ 5 AuthorsXiao-Ling Shen7
Estimated H-index: 7
(Guangzhou University of Chinese Medicine)
ABSTRACT BACKGROUND : Visceral obesity and fatty liver are prevalent in postmenopausal women. The stilbene-rich extract of Cajanus cajan (L.) Millsp. has been reported to prevent ovariectomy-induced and diet-induced weight gain in animal models, and stilbenoids from C. cajan are thought to have the potential to prevent postmenopausal obesity and fatty liver. PURPOSE : Cajanolactone A (CLA) is the main stilbenoid from C. cajan with osteoblastogenic promoting activity. This study investigated the potential of CLA to prevent postmenopausal obesity and fatty liver. Underlying mechanisms were also investigated. METHOD : Ovariectomized C57BL/6 mice fed a regular diet were used as mimics of postmenopausal women and given 10, 20, or 40 mg/kg/d of CLA, 0.1 mg/kg/d of estradiol valerate (EV, positive control), or vehicle (OVX) orally for 16 weeks. Mice of the same age subjected to a sham operation were used as control (Sham). Body weights were recorded every 2 weeks for 16 weeks. Body compositions were analyzed via micro-CT. Serum levels of lipids, adipocytokines and aminotransferases were measured using the relevant kits. mRNA levels of genes of interest were detected by RT-qPCR. Proteomic study of perigonadal white adipose tissue (pWAT) was performed using tandem-mass-tags-based proteomic technology combined with Parallel-Reaction-Monitoring (PRM) validation. RESULTS : CLA showed potential equivalent to that of EV to prevent ovariectomy-induced overweight, obesity, dyslipidemia, liver steatosis and liver dysfunction, but did not prevent uterine atrophy. In the liver, CLA significantly inhibited ovariectomy-induced upregulation in expression of lipogenic genes SREBP-1c and ChREBP, and stimulated the mRNA expression of apolipoprotein B gene ApoB. In pWAT, CLA reversed, or partially reversed ovariectomy-induced downregulation in the expression of a number of metabolism- and mitochondrial-function-related proteins, including Ndufa3, Pcx, Pdhb, Acly, Acaca, Aldh2, Aacs and Echs1. In addition, ovariectomy-inhibited mRNA expression of Pdhb, Aacs, Acsm5, Echs1, and Aldh2 genes in pWAT was also reversed. CONCLUSION : CLA was demonstrated to be a potential non-estrogen-like drug candidate for prevention of postmenopausal obesity and fatty liver. The underlying mechanism might involve the inhibition of lipogenesis and promotion of triglycerides output in the liver, and the promotion of metabolism and mitochondrial functions of visceral white adipose tissue.
📖 Papers frequently viewed together
3 Authors (Yoon-Hee Lee, ..., Mi-Kyung Sung)
4 Citations
#1Zhuang Lu (NAU: Nanjing Agricultural University)H-Index: 7
#2Xiaofang He (NAU: Nanjing Agricultural University)H-Index: 7
Last. Fei Gao (NAU: Nanjing Agricultural University)H-Index: 15
view all 8 authors...
ABSTRACT Chronic heat stress can enhance fat synthesis in broilers, and excessive triglyceride (TG) synthesized by the liver needs to be transported to extrahepatic tissues by very low density lipoprotein (VLDL) otherwise will accumulate in the liver, which may even result in hepatic steatosis. To investigate the molecular mechanisms by which chronic heat stress enhances fat synthesis and results in lipid accumulation in the liver of chickens, 144 broilers (Arbor Acres, 28-day-old) were randomly...
9 CitationsSource
#1Hsin-Hsueh Shen (NDMC: National Defense Medical Center)H-Index: 6
#2Shieh-Yang HuangH-Index: 2
Last. Yen-Mei Lee (NDMC: National Defense Medical Center)H-Index: 21
view all 8 authors...
Abstract Estrogen deficiency in postmenopausal women is linked to the higher prevalence of obesity, type 2 diabetes and metabolic syndromes. Development of beige adipocytes (browning of WAT) increases energy expenditure and could be a promising strategy for obesity management. This study aimed to investigate the effects of phytoestrogen genistein (GEN) on white adipose tissue (WAT) inflammation, browning and hepatic lipogenesis in ovariectomized rats with high-fat diet (HFD) and further explore ...
17 CitationsSource
#1Shan Liu (Guangzhou University of Chinese Medicine)H-Index: 3
#2Zhuo-Hui Luo (Guangzhou University of Chinese Medicine)H-Index: 2
Last. Xiao-Ling Shen (Guangzhou University of Chinese Medicine)H-Index: 7
view all 9 authors...
Cajanolactone A (CLA) is a stilbenoid discovered by us from Cajanus cajan (L.) Millsp. In our study, CLA was found to promote osteoblast differentiation in human bone marrow mesenchymal stem cells (hBMSCs), as judged by increased cellular alkaline phosphatase activity and extracellular calcium deposits, and elevated protein expression of Runx2, collagen-1, bone morphogenetic protein-2, and osteopontin. Mechanistic studies revealed that hBMSCs undergoing osteoblast differentiation expressed upreg...
3 CitationsSource
#1Radosław Słopień (Poznan University of Medical Sciences)H-Index: 8
#2Ewa Wender-Ozegowska (Poznan University of Medical Sciences)H-Index: 17
Last. Irene Lambrinoudaki (UoA: National and Kapodistrian University of Athens)H-Index: 32
view all 21 authors...
INTRODUCTION Whether menopause increases the risk of type 2 diabetes mellitus (T2DM) independently of ageing has been a matter of debate. Controversy also exists about the benefits and risks of menopausal hormone therapy (MHT) in women with T2DM. AIMS To summarise the evidence on 1) the effect of menopause on metabolic parameters and the risk of T2DM, 2) the effect of T2DM on age at menopause, 3) the effect of MHT on the risk of T2DM, and 4) the management of postmenopausal women with T2DM. MATE...
28 CitationsSource
#1Yang-Quan Hao (Xi'an Jiaotong University)H-Index: 2
#2Hao Guo (Xi'an Jiaotong University)H-Index: 3
Last. Puwei Yuan (Shaanxi University of Chinese Medicine)H-Index: 2
view all 7 authors...
The aim of this study was to evaluate the clinical effect of core decompression (CD), lesion clearance, and bone graft in combination with Tongluo Shenggu decoction for the treatment of osteonecrosis of the femoral head (ONFH). A total of 75 patients (92 hips), with ONFH at Association Research Circulation Osseous (ARCO) stages II to IIIA, were studied and divided into treatment group and control group. In control group, patients were treated with the CD in combination with autologous or artific...
2 CitationsSource
#1Alba Signes (MRC Mitochondrial Biology Unit)H-Index: 3
#2Erika Fernandez-Vizarra (MRC Mitochondrial Biology Unit)H-Index: 25
The assembly of the five oxidative phosphorylation system (OXPHOS) complexes in the inner mitochondrial membrane is an intricate process. The human enzymes comprise core proteins, performing the catalytic activities, and a large number of ‘supernumerary’ subunits that play essential roles in assembly, regulation and stability. The correct addition of prosthetic groups as well as chaperoning and incorporation of the structural components require a large number of factors, many of which have been ...
69 CitationsSource
#1Vandana GuptaH-Index: 4
#2Sameeksha Mishra (King George's Medical University)H-Index: 4
Last. Vani Gupta (King George's Medical University)H-Index: 7
view all 5 authors...
Abstract Leptin and adiponectin play an important role in the regulation of body weight and energy homeostasis. The purpose of the present study was to ascertain the relationship between leptin to adiponectin ratio (L:A) and metabolic risk factors in postmenopausal women.This is a cross sectional case-control study. A total of 523 postmenopausal women were recruited for the study 270 postmenopausal women with metabolic syndrome and 253 apparently healthy control postmenopausal women without meta...
7 CitationsSource
#1Clark M. HendersonH-Index: 14
#2Nicholas J. ShulmanH-Index: 11
Last. Andrew N. Hoofnagle (UW: University of Washington)H-Index: 56
view all 5 authors...
To the Editor: With the continuing expansion of mass spectrometry into the clinical laboratory for the quantification of proteins and small molecules, laboratories are being equipped with different instrument platforms from different manufacturers. This requires specific training for laboratory personnel on each data analysis software package that is unique to each instrument, which can increase method deployment time and potentially increase errors in data analysis. Skyline is a freely availabl...
17 CitationsSource
#1Ningjian Wang (SJTU: Shanghai Jiao Tong University)H-Index: 18
#2Hongfang Shao (SJTU: Shanghai Jiao Tong University)H-Index: 1
Last. Yingli Lu (SJTU: Shanghai Jiao Tong University)H-Index: 20
view all 9 authors...
Background Cardiovascular disease is the leading cause of mortality in postmenopausal women. Follicle‐stimulating hormone (FSH) shows negative associations with obesity and diabetes mellitus in postmenopausal women. We aimed to study the associations between FSH and 10‐year risk of atherosclerotic cardiovascular disease (ASCVD) in postmenopausal women. Methods and Results SPECT‐China (the Survey on Prevalence in East China for Metabolic Diseases and Risk Factors) is a 22‐site, population‐based s...
23 CitationsSource
#1Ashkan Afshin (UW: University of Washington)H-Index: 42
#2Mohammad H. Forouzanfar (UW: University of Washington)H-Index: 64
Last. Christopher J L MurrayH-Index: 182
view all 153 authors...
BACKGROUND Although the rising pandemic of obesity has received major attention in many countries, the effects of this attention on trends and the disease burden of obesity remain uncertain. METHOD ...
2,032 CitationsSource
Cited By0