Pre-Clinical Assessment of Single-Use Negative Pressure Wound Therapy During In Vivo Porcine Wound Healing.

Published on Nov 13, 2020in Advances in wound care2.813
· DOI :10.1089/WOUND.2020.1218
Varuni R. Brownhill1
Estimated H-index: 1
(Smith & Nephew),
Elizabeth Mary Huddleston6
Estimated H-index: 6
(Smith & Nephew)
+ 4 AuthorsHolly N. Wilkinson8
Estimated H-index: 8
(Hull York Medical School)
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Abstract
Objective: Traditional negative pressure wound therapy systems can be large and cumbersome, limiting patient mobility and adversely affecting quality of life. PICO™, a no canister single-use system offers a lightweight, portable alternative to traditional negative pressure wound therapy, with improved clinical performance. The aim of this study was to determine the potential mechanism(s) of action of single-use negative pressure wound therapy versus traditional negative pressure wound therapy. Approach: Single-use negative pressure wound therapy and traditional negative pressure wound therapy were applied to an in vivo porcine excisional wound model, following product use guidelines. Macroscopic, histological and biochemical analyses were performed at defined healing time-points to assess multiple aspects of the healing response. Results: Wounds treated with single-use negative pressure displayed greater wound closure and increased re-epithelialisation versus those treated with traditional negative pressure. The resulting granulation tissue was more advanced with fewer neutrophils, reduced inflammatory markers, more mature collagen and no wound filler-associated foreign body reactions. Of note, single-use negative pressure therapy failed to induce wound edge epithelial hyperproliferation, while traditional negative pressure therapy compromised peri-wound skin, which remained inflamed with high transepidermal water loss; features not observed following single-use treatment. Innovation: Single-use negative pressure was identified to improve multiple aspects of healing versus traditional negative pressure treatment. Conclusion: This study provides important new insight into the differing mode of action of single-use versus traditional negative pressure and may go some way to explaining the improved clinical outcomes observed with single use negative pressure therapy.
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#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Francesca L. Longhorne (Hull York Medical School)
Last. Matthew J. Hardman (Hull York Medical School)H-Index: 34
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Negative pressure wound therapy is a widely used treatment for chronic, nonhealing wounds. Surprisingly, few studies have systematically evaluated the cellular and molecular effects of negative pressure treatment on human skin. In addition, no study to date has directly compared recently available single-use negative pressure modalities to traditional negative pressure devices in a controlled setting. Here we developed a novel large-scale ex vivo human skin culture system to effectively evaluate...
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#1Holly N. Wilkinson (Hull York Medical School)H-Index: 8
#2Matthew J. Hardman (Hull York Medical School)H-Index: 34
Wound healing is a complex, dynamic process supported by a myriad of cellular events that must be tightly coordinated to efficiently repair damaged tissue. Derangement in wound-linked cellular beha...
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