Systems Glycoengineering: Integrated Analyses of Glycomics, Transcriptomics and Phenotypic Data Reveal Complex Cellular Response to Glycoengineering in CHO Cells

Glycosylation is fundamentally important to the activity, stability and pharmacokinetics of biopharmaceuticals. Move on to the future, glycoengineering is a promising way to improve protein drug efficacy. Recently, several major advances in engineering efforts have successfully led to desired protein glycoforms, but some desirable glycoengineering designs have proven toxic to the host cells (e.g., CHO cell lines). Up to date, the critical...