Deformable molecularly imprinted nanogels permit sensitivity-gain in plasmonic sensing.

Published on May 15, 2020in Biosensors and Bioelectronics10.257
· DOI :10.1016/J.BIOS.2020.112126
Nunzio Cennamo18
Estimated H-index: 18
Devid Maniglio24
Estimated H-index: 24
(University of Trento)
+ 2 AuthorsAlessandra Bossi28
Estimated H-index: 28
(University of Verona)
Abstract Soft molecularly imprinted nanogels (nanoMIPs), selective for human transferrin (HTR), were prepared via a template assisted synthesis. Owing to their soft matter, the nanoMIPs were observed to deform at binding to HTR: while no relevant changes were observed in the hydrodynamic sizes of HTR-free compared to HTR-loaded nanoMIPs, the HTR binding resulted in a significant increment of the nanoMIP stiffness, with the mean Young's modulus measured by AFM passing from 17 ± 6 kPa to 56 ± 18 kPa. When coupled to a plastic optical fibre (POF) plasmonic platform, the analyte-induced nanoMIP-deformations amplified the resonance shift, enabling to attain ultra-low sensitivities (LOD = 1.2 fM; linear dynamic range of concentrations from 1.2 fM to 1.8 pM). Therefore, soft molecularly imprinted nanogels that obey to analyte-induced deformation stand as a novel class of sensitivity-gain structures for plasmonic sensing.
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