Original paper
Mapping axon initial segment structure and function by multiplexed proximity biotinylation
Abstract
Axon initial segments (AISs) generate action potentials and regulate the polarized distribution of proteins, lipids, and organelles in neurons. While the mechanisms of AIS Na+ and K+ channel clustering are understood, the molecular mechanisms that stabilize the AIS and control neuronal polarity remain obscure. Here, we use proximity biotinylation and mass spectrometry to identify the AIS proteome. We target the biotin-ligase BirA* to the AIS by...
Paper Details
Title
Mapping axon initial segment structure and function by multiplexed proximity biotinylation
Published Date
Jan 3, 2020
Journal
Volume
11
Issue
1