The effects of non-esterified fatty acids and β-hydroxybutyrate on the hepatic CYP2E1 in cows with clinical ketosis.

Published on Feb 1, 2019in Journal of Dairy Research1.628
· DOI :10.1017/S0022029919000025
Zhicheng Peng9
Estimated H-index: 9
,
Xiaobing Li22
Estimated H-index: 22
+ 2 AuthorsXinwei Li23
Estimated H-index: 23
Source
Abstract
: Dairy cows with ketosis display severe oxidative stress as well as high blood concentrations of non-esterified fatty acids (NEFA) and β-hydroxybutyrate (BHB). Cytochrome P4502E1 (CYP2E1) plays an important role in the induction of oxidative stress. The aim of this study was to investigate CYP2E1 expression and activity in the liver of clinically ketotic cows (in vivo) and the effects of NEFA and BHB on CYP2E1 expression and activity in hepatocytes (in vitro). Dairy cows with clinical ketosis exhibited a low blood concentration of glucose but high concentrations of NEFA and BHB. Hepatic mRNA, protein expression, and activity of CYP2E1 were significantly higher in cows with clinical ketosis than in control cows. In vitro, both NEFA and BHB treatment markedly up-regulated the mRNA and protein expressions as well as activity of CYP2E1 in cow hepatocytes. Taken together, these results indicate that high levels of NEFA and BHB significantly up-regulate the expression and activity of hepatic CYP2E1, and may be influential in the induction of oxidative stress in cows with clinical ketosis.
References18
Newest
#1Xiliang Du (JLU: Jilin University)H-Index: 12
#2Yiwei Zhu (JLU: Jilin University)H-Index: 9
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#1Xiliang Du (JLU: Jilin University)H-Index: 12
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