A Suicide Switch Directly Eliminates Intracellular scFv Oligomers in the Cytoplasm of Mammalian Cells

As intracellular antibodies (intrabodies) are highly promising tools for drug discovery, an innovative antibody screening platform in mammalian cells was previously developed by a single‐chain Fv (scFv)‐c‐kit growth sensor, which successfully selected rabies nucleoprotein and phosphoprotein‐specific intrabodies from a synthetic scFv library. Since the scFv‐c‐kit growth sensor releases a growth signal after forming oligomers due to binding to an...