Teruyuki Nagamune
University of Tokyo
Fusion proteinBiophysicsAntigenCellMolecular biologyAntibodyReceptorEnzymeChemistryPeptideImmunoassayChimera (genetics)Erythropoietin receptorBiochemistrySignal transductionStereochemistryIntracellularCell cultureBiologyCell biology
399Publications
39H-index
6,080Citations
Publications 356
Newest
#1Satoshi Yamaguchi (UTokyo: University of Tokyo)H-Index: 19
#2Yumi Takasaki (UTokyo: University of Tokyo)H-Index: 1
Last. Teruyuki Nagamune (UTokyo: University of Tokyo)H-Index: 39
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Source
#1Kosuke Minamihata (Kyushu University)H-Index: 10
#2K. Tsukamoto (UTokyo: University of Tokyo)
Last. Teruyuki Nagamune (UTokyo: University of Tokyo)H-Index: 39
view all 7 authors...
We utilized electrostatic interaction to induce rapid crystallization of streptavidin. Simply mixing streptavidins possessing either a positively or negatively charged peptide at their C-terminus generated diffraction-quality crystals in a few hours. We modified the streptavidin crystals with fluorescent molecules using biotin, demonstrating the concept of protein crystals as functional biomaterials.
1 CitationsSource
#1Tasuku Tsukamoto (Rits: Ritsumeikan University)H-Index: 2
#2Takahiro Sogo (Rits: Ritsumeikan University)H-Index: 7
Last. Teruhisa Kawamura (Rits: Ritsumeikan University)H-Index: 11
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: Producing a sufficient number of cardiomyocytes from pluripotent stem cells has been of great demand for cardiac regeneration therapy. However, it remains challenging to efficiently differentiate cardiomyocytes with low costs. Reportedly, granulocyte colony-stimulating factor (G-CSF) receptor (GCSFR) signaling activates signal transducers and activators of transcription (STAT) signaling and enhances cardiac differentiation from embryonic stem cells or induced pluripotent stem cells (iPSCs). To...
3 CitationsSource
#1Natalia Teresa Jarzębska (UTokyo: University of Tokyo)H-Index: 1
#2Satoshi Yamaguchi (UTokyo: University of Tokyo)H-Index: 19
Last. Akimitsu Okamoto (UTokyo: University of Tokyo)H-Index: 32
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We report a photo-cleavable material for tight trapping of nonadherent cells to substrate surfaces. Model immunocytes were selectively trapped in a non-irradiated area as single cells after the projection of a light pattern and withstood high-speed laminar flow, achieving light-guided cell release from the substrates.
1 CitationsSource
#1Ayumu Ishijima (UTokyo: University of Tokyo)H-Index: 3
#2Satoshi Yamaguchi (UTokyo: University of Tokyo)H-Index: 19
Last. Ichiro Sakuma (UTokyo: University of Tokyo)H-Index: 27
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#1Akihiro Eguchi (UTokyo: University of Tokyo)H-Index: 1
#2Makoto Nakakido (UTokyo: University of Tokyo)H-Index: 8
Last. Masahiro Kawahara (UTokyo: University of Tokyo)H-Index: 16
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: Upon developing therapeutically potent antibodies, there are significant requirements, such as increasing their affinity, regulating their epitope, and using native target antigens. Many antibody selection systems, such as a phage display method, have been developed, but it is still difficult to fulfill these requirements at the same time. Here, we propose a novel epitope-directed antibody affinity maturation system utilizing mammalian cell survival as readout. This system is based on the comp...
2 CitationsSource
#1Thuy Duong Nguyen (UTokyo: University of Tokyo)H-Index: 1
#2Teruyuki Nagamune (UTokyo: University of Tokyo)H-Index: 39
Last. Masahiro Kawahara (UTokyo: University of Tokyo)H-Index: 16
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: As intracellular antibodies (intrabodies) are highly promising tools for drug discovery, an innovative antibody screening platform in mammalian cells was previously developed by a single-chain Fv (scFv)-c-kit growth sensor, which successfully selected rabies nucleoprotein and phosphoprotein-specific intrabodies from a synthetic scFv library. Since the scFv-c-kit growth sensor releases a growth signal after forming oligomers due to binding to an oligomeric antigen, it is critical to use a libra...
1 CitationsSource
#1Tomoaki HagaH-Index: 3
#2Hidehiko HirakawaH-Index: 12
Last. Teruyuki NagamuneH-Index: 39
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: Most bacterial cytochrome P450 monooxygenases (P450s) do not work alone because their active species is generated by two electrons supplied through two separate auxiliary proteins. Artificial "self-sufficient" P450s, in which one molecule each of the two auxiliary proteins is arranged close to the P450s, have been developed but have not achieved the maximum catalytic turnover numbers of the P450s. In this study, the Pseudomonas putida P450 (P450cam) is assembled with multiple molecules of its ...
3 CitationsSource
#1Fumiya IwataH-Index: 2
#2Hidehiko HirakawaH-Index: 12
Last. Teruyuki NagamuneH-Index: 39
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: Bacterial cytochrome P450 monooxygenases (P450s) are promising biocatalysts for chemical syntheses because they catalyze a variety of oxidations on non-activated hydrocarbons using O2 . However, the requirement of two auxiliary proteins, an electron transfer protein and a reductase, for the catalysis is a major bottleneck for in vitro applications of these monooxygenases. The authors previous study showed that artificial assembly of a bacterial P450 with its auxiliary proteins using a heterotr...
2 CitationsSource
#1Modong Tan (UTokyo: University of Tokyo)H-Index: 3
#2Satoshi Yamaguchi (UTokyo: University of Tokyo)H-Index: 19
Last. Teruyuki Nagamune (UTokyo: University of Tokyo)H-Index: 39
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G-protein coupled receptors (GPCRs) are involved in many diseases and important biological phenomena; elucidating the mechanisms underlying regulation of their signal transduction potentially provides both novel targets for drug discovery and insight into living systems. A proton-sensing GPCR, ovarian cancer G protein-coupled receptor 1 (OGR1), has been reported to be related to acidosis and diseases that cause tissue acidification, but the mechanism of proton-induced activation of OGR1-mediated...
10 CitationsSource
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