Abstract In traditional Chinese medicine practice, crude herbs are often subjected to traditional processing (Paozhi in Chinese) for a special medicinal purpose. Bran-frying is one of processing methods for Paeoniae Radix Alba (PRA). Previous studies found that paeoniflorin and paeoniflorin sulfonate, a principle bioactive compound and its sulfur-fumigation induced characteristic sulfur-containing derivative, could be used together with sulfur dioxide residue as chemical markers for the quality control of sulfur-fumigated PRA crude material. In this paper, the feasibility of these three markers used for the quality control of bran-fried sulfur-fumigated PRA was further investigated. First, homemade samples of sulfur-fumigated PRA with different sulfur-fumigation duration (0.5–6 h) were bran-fried, and stored for 12 months. Second, the contents of sulfur dioxide residue, paeoniflorin and paeoniflorin sulfonate were dynamically quantified respectively. Third, the variation of the marker contents and their correlation during bran-frying and storage was evaluated. A validation was conducted using commercial bran-fried PRA samples. The results showed that bran-frying caused an averaged reduction of 20% in the content of sulfur dioxide residue, and during the first two months of storage the content of sulfur dioxide residue was decreased by up to 27%, then the content was tending towards stability for the subsequent ten months of storage (RSD = 3.92%). Meanwhile, paeoniflorin and paeoniflorin sulfonate were relatively stable, the contents of which were not affected by bran-frying processing and 12 months of storage. The correlations between the contents of sulfur dioxide residue and paeoniflorin/paeoniflorin sulfonate were obviously influenced by storage duration. Since sulfur dioxide residue is a safety marker, while paeoniflorin and paeoniflorin sulfonate can reflect the inner quality and the impact extent of sulfur-fumigation on the quality of bran-fried PRA respectively, these three chemicals might be used together as markers for the quality control, and consequently to ensure the safety and efficacy of bran-fried PRA.
#1Ming Kong(Nanjing University of Chinese Medicine)H-Index: 11
#2Huan-huan Liu(Nanjing University of Chinese Medicine)H-Index: 2
Last. Song-Lin Li(Nanjing University of Chinese Medicine)H-Index: 26
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Abstract Ethnopharmacological relevance Radix Paeoniae Alba ( Baishao , BS), one of the most commonly used traditional Chinese medicinal herbs, has many pharmacological effects including analgesic activity. Previous studies found that sulfur-fumigation, a post-harvest handling process developed to prevent mold contamination of medicinal herbs, altered the quality of BS. However, whether sulfur-fumigation affects the pharmacokinetics, safety and efficacy of BS warrants further investigation. Aim ...
#1Li Zhou(Nanjing University of Chinese Medicine)H-Index: 4
#2Jin-Di Xu(Nanjing University of Chinese Medicine)H-Index: 11
Last. Song-Lin Li(Nanjing University of Chinese Medicine)H-Index: 26
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Abstract Exploring processing chemistry, in particular the chemical transformation mechanisms involved, is a key step to elucidate the scientific basis in traditional processing of herbal medicines. Previously, taking Rehmanniae Radix (RR) as a case study, the holistic chemome (secondary metabolome and glycome) difference between raw and processed RR was revealed by integrating hyphenated chromatographic techniques-based targeted glycomics and untargeted metabolomics. Nevertheless, the complex c...
#1Li Zhou(Nanjing University of Chinese Medicine)H-Index: 4
#2Jin-Di Xu(Nanjing University of Chinese Medicine)H-Index: 11
Last. Song-Lin Li(Nanjing University of Chinese Medicine)H-Index: 26
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Abstract It is well-recognized that multiple components, the majority of which are secondary metabolites and carbohydrates, collectively contribute to the therapeutic effects of herbal medicines. The chemical characterization of herbal medicines has focused extensively on secondary metabolites but has largely overlooked carbohydrates. Here, we proposed an integrated chromatographic technique based targeted glycomics and untargeted metabolomics strategy simultaneously determining carbohydrates an...
The content of SO2 in Paeoniae Radix Alba( RPA) was determined by the method documented in Chinese Pharmacopoeia( CP) 2010 edition to validate the repeatability of the method for evaluating RPA,and the contents of paeoniflorin sulfonate in both the residual material and distilled solution of RPA were determined by HPLC to study the transformation of paeoniflorin sulfonate to SO2 by HCl. It was found that the repeatability of the method in CP for evaluating RPA is unacceptable,and paeoniflorin su...
Abstract In this study, a improved high performance liquid chromatography–diode array detector (HPLC–DAD) method for simultaneous quantification of twelve major components belonging to three chemical types was developed and validated, and was applied to quantitatively compare the quality of Radix Paeoniae Alba (RPA) sulfur-fumigated with different durations and purchased from commercial herbal markets. The contents of paeoniflorin, benzoylpaeoniflorin, oxypaeoniflorin, benzoic acid and paeonol d...
#1Xue Jiang(Peking Union Medical College)H-Index: 6
#2Linfang Huang(Peking Union Medical College)H-Index: 2
Last. Shilin Chen(Peking Union Medical College)H-Index: 64
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Abstract Sulfur fumigation (SF) in Traditional Chinese Medicine (TCM) is a highly efficient and important traditional preservation method in China. This method has generated a great deal of concern and has been disputed in the last few years because of its uncertain safety. SF can alter the quality of TCMs by damaging the bioactive compounds, changing chemical profiles, and generating detrimental exogenous materials. However, SF is still widely used in the herbal medicinal industry because of it...
Detection of sulfur-fumigated Paeoniae Alba Radix (PAR) in different complex preparations is challenging due to the relatively lower content of PAR and interference from more complicated components in complex preparations with different multiple constituent herbs. In this study, a high performance liquid chromatography- triple-quadrupole tandem mass spectrometry method was developed for detecting sulfur-fumigated PAR in different complex preparations. Paeoniflorin, the major component of PAR, an...
#2Neil L. A. Misso(UWA: University of Western Australia)H-Index: 26
Sulphites are widely used as preservative and antioxidant additives in the food and pharmaceutical industries. Exposure to sulphites has been reported to induce a range of adverse clinical effects in sensitive individuals, ranging from dermatitis, urticaria, flushing, hypotension, abdominal pain and diarrhoea to life-threatening anaphylactic and asthmatic reactions. Exposure to the sulphites arises mainly from the consumption of foods and drinks that contain these additives; however exposure may...
Abstract Aim White peony root and sulfur fumigated white peony root are produced by different processing methods from the root of Paeonia lactiflora Pall, but the two traditional Chinese medicines are used under the same common name white peony root. In order to clarify the influence of sulfur fumigation on pharmacokinetics of the main monoterpene glucoside components in white peony root, an investigation was carried out to compare the pharmacokinetics of sodium paeoniflorin sulfonate ( 1 ) and ...
#1Liu Yan(CDUTCM: Chengdu University of Traditional Chinese Medicine)H-Index: 1
OBJECTIVE:To discuss the status quo and the development strategy of the study of the processing of Chinese herbal medicine.METHODS:The physical-chemical property,the technology transfer and the qualitation and quantitation reported in the literature about the processing of Chinese herbal medicine were analyzed and summarized.The status quo and the current methods for the study of processing of Chinese herbal medicine were expounded and its development strategy was put forward in view of the role...
Sulfur fumigation is adopted in the post-harvest processing of herbal drugs. In addition to the sulfur dioxide residual, sulfur fumigation could also lead to chemical transformations of native compounds in the herbs to generate sulfur-containing derivatives. In this study, influence of sulfur fumigation on the chemical composition of Farfarae Flos (FF) was investigated by the ultra-high-performance liquid chromatography-Q exactive hybrid quadrupole-orbitrap high-resolution accurate mass spectrom...
Last. Weidong Zhang(SHU: Shanghai University)H-Index: 58
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Abstract Ethnopharmacological relevance Aconiti Lateralis Radix Praeparata (the Chinese name is Fuzi, FZ), the lateral or daughter root of Aconitum carmichaelii Debx. (Ranunculaceae), is a controversial traditional Chinese medicine (TCM) that is universally distributed and applied in many countries, such as China, Japan, Korea, and India. FZ can be used to treat various diseases, including rheumatic fever, rheumatism, painful joints, syncope, collapse, bronchial asthma, some endocrinal disorders...
The traditional drying method, sun drying, for Chrysanthemum morifolium Ramat. cv. Hang-ju (Compositae) (HJ) is widely replaced by sulphur fumigation (SF), which has an unknown effect on its effica...
Last. Weidong Zhang(SHU: Shanghai University)H-Index: 58
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Chinese materia medica processing is a distinguished and unique pharmaceutical technique in traditional Chinese medicine (TCM), which has played an important role in reducing side effects, increasing medical potencies, altering the properties and even changing the curative effects of raw herbs. The efficacy improvement in medicinal plants is mainly caused by changes in the key substances through an optimized processing procedure. Thus, the use of a rapid method for determining suitable chemical ...