Fast and Binary Assay for Predicting Radiosensitivity Based on the Theory of ATM Nucleo-Shuttling: Development, Validation, and Performance.

Published on Feb 1, 2018in International Journal of Radiation Oncology Biology Physics5.859
· DOI :10.1016/J.IJROBP.2017.10.029
Sandrine Pereira23
Estimated H-index: 23
(French Institute of Health and Medical Research),
Larry Bodgi8
Estimated H-index: 8
(French Institute of Health and Medical Research)
+ 7 AuthorsNicolas Foray30
Estimated H-index: 30
(French Institute of Health and Medical Research)
Purpose To examine the possibility of predicting clinical radiosensitivity by quantifying the nuclear forms of autophosphorylated ATM protein (pATM) via a specific enzyme-linked immunosorbent assay (ELISA). Methods and Materials This study was performed on 30 skin fibroblasts from 9 radioresistant patients and 21 patients with adverse tissue reaction events. Patients were divided into 2 groups: radioresistant (toxicity grade Results The pATM ELISA data were in quantitative agreement with the immunofluorescence data. A receiver operating characteristic analysis was applied first to 2 data sets (a training set [n=14] and a validating [n=16] set) and thereafter to all the data with a 2-fold cross-validation method. The assay showed an area under the curve value higher than 0.8, a sensitivity of 0.8, and a specificity ranging from 0.75 to 1, which strongly documents the predictive power of the pATM ELISA. Conclusion This study showed that the assessment of nuclear pATM quantity after 2 Gy via an ELISA technique can be the basis of a predictive assay with the highest statistical performance among the available predictive approaches.
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