Structural basis of the redox switches in the NAD ⁺ -reducing soluble [NiFe]-hydrogenase

How a hydrogenase protects its active site Hydrogen-metabolizing organisms use an [NiFe]-hydrogenase to catalyze hydrogen oxidation. One type of [NiFe]-hydrogenase, the NAD + -reducing soluble [NiFe]-hydrogenase (SH), couples reduction of NAD + to the oxidation of hydrogen. Shomura et al. solved the structure of SH from an H 2 -oxidizing bacterium in both the air-oxidized and the active reduced state. In the reduced state, the NiFe catalytic...