Fabrication of Hierarchical Macroporous Biocompatible Scaffolds by Combining Pickering High Internal Phase Emulsion Templates with Three-Dimensional Printing
Biocompatible and biodegradable porous scaffolds with adjustable pore structure have aroused increasing interest in bone tissue engineering. Here, we report a facile method to fabricate hierarchical macroporous biocompatible (HmPB) scaffolds by combining Pickering high internal phase emulsion (HIPE) templates with three-dimensional (3D) printing. HmPB scaffolds composed of a polymer matrix of poly(l-lactic acid), PLLA, and poly(e-caprolactone), PCL, are readily fabricated by solvent evaporation of 3D printed Pickering HIPEs which are stabilized by hydrophobically modified silica nanoparticles (h-SiO2). The pore structure of HmPB scaffolds is easily tailored to be similar to natural extracellular matrix (ECM) by varying the fabrication conditions of the Pickering emulsion or adjusting the printing parameters. In addition, in vivo drug release studies which employ enrofloxacin (ENR) as a model drug indicate the potential of HmPB scaffolds as a drug carrier. Furthermore, in vivo cell culture assays prove tha...
Scaffold of gelatin-chondroitin sulfate-hyaluronan-polyvinyl alcohol (GCH-PVA) with orientated microtubule structure and good hydrophilicity was fabricated by unidirectional freeze-drying method mimicking the composition and structure of tracheal cartilage extracellular matrix. PVA was incorporated to improve flexibility and viscoelasticity of GCH scaffold. All wet scaffolds showed similar compressive elastic modulus with native cartilage. GCH-PVA scaffolds showed high relative remaining stress ...
3D printing via direct ink writing (DIW) is a versatile additive manufacturing approach applicable to a variety of materials ranging from ceramics over composites to hydrogels. Due to the mild processing conditions compared to other additive manufacturing methods, DIW enables the incorporation of sensitive compounds such as proteins or drugs into the printed structure. Although emulsified oil-in-water systems are commonly used vehicles for such compounds in biomedical, pharmaceutical, and cosmet...
Hierarchical cellular structures are ubiquitous in nature because of their low-density, high-specific properties, and multifunctionality. Inspired by these systems, we created lightweight ceramic architectures composed of closed-cell porous struts patterned in the form of hexagonal and triangular honeycombs by direct foam writing. The foam ink contains bubbles stabilized by attractive colloidal particles suspended in an aqueous solution. The printed and sintered ceramic foam honeycombs possess l...
#2Yong Lei(SHNU: Shanghai Normal University)H-Index: 7
Last. Changqing Zhang(SJTU: Shanghai Jiao Tong University)H-Index: 52
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The properties of bone scaffolds, including biocompatibility, osteoinductivity and antibacterial activity, are of great importance for reconstruction of large bone defects and prevention of implant-associated infections. Herein, we develop an Ag-loaded strontium hydroxyapatite (SrHAP)/chitosan (CS) porous scaffold (Ag–SrHAP/CS) according to the following steps: (i) freeze-drying fabrication of a SrHAP/CS porous scaffold; and (ii) deposition of Ag nanoparticles on the above scaffold. In addition,...
: Bulk hierarchical porous ceramics with unprecedented strength-to-weight ratio and tunable pore sizes across three different length scales are printed by direct ink writing. Such an extrusion-based process relies on the formulation of inks in the form of particle-stabilized emulsions and foams that are sufficiently stable to resist coalescence during printing.
Last. GeunHyung Kim(SKKU: Sungkyunkwan University)H-Index: 46
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Recently, a three-dimensional (3D) bioprinting process for obtaining a cell-laden structure has been widely applied because of its ability to fabricate biomimetic complex structures embedded with and without cells. To successfully obtain a cell-laden porous block, the cell-delivering vehicle, bioink, is one of the significant factors. Until now, various biocompatible hydrogels (synthetic and natural biopolymers) have been utilized in the cell-printing process, but a bioink satisfying both biocom...
Design and preparation of high-performance immobilized biocatalysts with exquisite structures and elucidation of their profound structure-performance relationship are highly desired for green and sustainable biotransformation processes. Learning from nature has been recognized as a shortcut to achieve such an impressive goal. Loose connective tissue, which is composed of hierarchically organized cells by extracellular matrix (ECM) and is recognized as an efficient catalytic system to ensure the ...
#1Guangzhao Zhang(SCUT: South China University of Technology)H-Index: 33
#2Chaoyang Wang(SCUT: South China University of Technology)H-Index: 113
Last. Chaoyang Wang(SCUT: South China University of Technology)H-Index: 41
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The biodegradable cellular capsule, being prepared from simple vaporization of liquid marbles, is an ideal vehicle for the potential application of drug encapsulation and release. This paper reports the fabrication of cellular capsules via facile vaporization of Pickering emulsion marbles in an ambient atmosphere. Stable Pickering emulsion (water in oil) was prepared while utilizing dichloromethane (containing poly(l-lactic acid)) and partially hydrophobic silica particles as oil phase and stabi...
Last. Craig L. Duvall(Vandy: Vanderbilt University)H-Index: 33
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Self-assembled polymer/porous silicon nanocomposites overcome intracellular and systemic barriers for in vivo application of peptide nucleic acid (PNA) anti-microRNA therapeutics. Porous silicon (PSi) is leveraged as a biodegradable scaffold with high drug-cargo-loading capacity. Functionalization with a diblock polymer improves PSi nanoparticle colloidal stability, in vivo pharmacokinetics, and intracellular bioavailability through endosomal escape, enabling PNA to inhibit miR-122 in vivo.
Three-dimensional (3D) printing has been a very active area of research and development due to its capability to produce 3D objects by design. Miniaturization and improvement of spatial resolution are major challenges in current 3D printing technology development. This work reports advances in miniaturizing 3D printing to the nanometer scale using scanning probe microscopy in conjunction with local material delivery. Using polyelectrolyte polymers and complexes, we have demonstrated the concept ...
Abstract null null In this study, high internal phase Pickering emulsions (HIPPEs) stabilized by protein-polysaccharide complexes were used as inks for food-grade three-dimensional printing (3DP). The complexes (RCs) structured by synergistic interactions between rice proteins (RPs) and carboxymethyl cellulose (CMC) displayed outstanding biphasic wettability with excellent ability to reduce the oil/water interfacial tension. The interfacial structures formed by RCs provided a steric barrier and ...
#1Guo Yongtai(ECUST: East China University of Science and Technology)
#2Shengmiao Zhang(ECUST: East China University of Science and Technology)H-Index: 13
Last. Yun Zhu(ECUST: East China University of Science and Technology)H-Index: 6
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Abstract null null Developing renewable porous materials with low thermal conductivity and high flame retardancy is of great significance in energy saving and fire safety. Herein, a series of bio-based anisotropic composite foams were fabricated from oil-in-water (o/w) high internal phase Pickering emulsions (Pickering HIPEs) stabilized by both bio-based poly (urethane-acrylate) (PUA) and poly (cyclotriphosphazene-co-4,4ʹ-sulfonyldiphenol) (PZS) particles. All Pickering HIPEs have good stability...
Bone tissue engineering commonly encompasses the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the propagation of cells to regenerate damaged tissues or organs. 3D printing technology has been extensively applied to allow direct 3D scaffolds manufacturing. Polycaprolactone (PCL) has been widely used in the fabrication of 3D scaffolds in the field of bone tissue engineering due to its advantages such as good biocompatibility, slow degradation rate, the less ac...
The shaping of metal-organic frameworks (MOFs), referring to the integration of small sub-millimeter MOF crystals into bulk samples of desired size and shape, is an important step in the practical use of this class of porous material in many applications. Herein, we demonstrate for the first time the fabrication of hierarchical 3D MOF monoliths in situ within an MOF particle-stabilized high internal phase emulsion (HIPE). In this approach, a subfamily MOF (ZIF-8) is selected as the sole Pickerin...
#1Shengmiao Zhang(ECUST: East China University of Science and Technology)
#2Pan Zhou(ECUST: East China University of Science and Technology)
Last. Zhang Ka(ECUST: East China University of Science and Technology)
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Abstract PolyHIPEs prepared by polymerizing high internal phase emulsions (HIPEs) normally have a cross-linked structure, with the cross-linking providing the mechanical stability to maintain the porous structure and prevent collapse during cure and uses. The most widely investigated polyHIPE (i.e., polystyrene (PS) polyHIPE) is cross-linked with divinylbenzene (DVB), making them rigid, brittle, and environmentally unfriendly. To address this issue, tough and recyclable PS based polyHIPEs are pr...
Last. Peter Krajnc(University of Maribor)H-Index: 30
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High internal phase emulsions (HIPEs), with densely packed droplets of internal phase and monomers dispersed in the continuous phase, are now an established medium for porous polymer preparation (polyHIPEs). The ability to influence the pore size and interconnectivity, together with the process scalability and a wide spectrum of possible chemistries are important advantages of polyHIPEs. In this review, the focus on the biomedical applications of polyHIPEs is emphasised, in particular the applic...
#1Ning Zhang(Anhui University of Technology)H-Index: 1
#2Yunfei Qi(Anhui University of Technology)H-Index: 1
Last. Wei Jiang(Nanjing University of Science and Technology)H-Index: 30
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Abstract Bottom-up and up-down strategy were adopted simultaneously to fabricate the interconnected porous polystyrene monolith based on the high internal phase emulsions (HIPEs) technique. Interface-initiated polymerization strategy of the HIPEs combined with porogen toluene molecule offered an attractive route to fabricate the ultra-light polystyrene foam. High porosity and extra-light density endowed the polystyrene foam with outstanding oil adsorption performance towards a wide range of oil/...
Last. Yuhao Zhang(SWU: Southwest University)H-Index: 18
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Abstract In this study, the gelatin crosslinked by Transglutaminase (TG) was applied to stabilize food-grade high internal phase emulsions (HIPEs) by a facile one-step method. The influence of TG crosslinking time on the molecular weight distribution of gelatin and the microstructure, thermal stability and rheological properties of the HIPEs were investigated. The results showed that TG crosslinking effectively promoted the production of high weight molecular. The obtained HIPEs exhibited a unif...
Last. Zhuohong Yang(SCAU: South China Agricultural University)H-Index: 15
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In this work, a 3D printed double-network (DN) hydrogel scaffold is designed with chitosan (CS) and polyvinyl alcohol (PVA) as the framework matrix. The addition of PVA into the CS-based hydrogel clearly enhances the mechanical properties and lowers the swelling behaviors of the hydrogels. The crosslinking of CS with genipin can perform the pre-crosslinking to improve the viscosity and 3D printability of the hydrogel precursor, while increasing the PVA content results in lowering the viscosity a...