Lipid Laden Macrophages in Respiratory Disease.

Published on Jul 30, 2017in Journal of Neurogastroenterology and Motility3.008
· DOI :10.5056/JNM17036
Laura R. Sadofsky12
Estimated H-index: 12
(Hull York Medical School),
Yvette A. Hayman3
Estimated H-index: 3
(Hull York Medical School)
+ 1 AuthorsAlyn H. Morice2
Estimated H-index: 2
(Hull York Medical School)
#1Pelin Özdemir (Ege University)H-Index: 2
#2Münevver Erdinç (Ege University)H-Index: 7
Last. Serhat Bor (Ege University)H-Index: 21
view all 7 authors...
Gastroesophageal reflux disease (GERD) is one of the main causes of chronic cough. We evaluated the role of microaspiration in the pathogenesis of reflux-related cough by determining the amount of lipid-laden macrophages (LLMs) in bronchoalveolar lavage (BAL) specimens.A total of 161 cases of chronic cough were evaluated, and 36 patients (average age 48.2 years) were recruited for this single center prospective study. Patients with a history of smoking, angiotensin converting enzyme inhibitor us...
13 CitationsSource
#1Yvette A. Hayman (Hull York Medical School)H-Index: 3
#2Laura R. Sadofsky (Hull York Medical School)H-Index: 12
Last. Alyn H. Morice (Hull York Medical School)H-Index: 35
view all 5 authors...
Chronic inflammatory diseases of the airways are associated with gastro-oesophageal reflux (GOR) and aspiration events. The observation of lipid laden macrophages (LLM) within the airway may indicate aspiration secondary to GOR. The proposed mechanism, that lipid droplets from undigested or partially digested food are aspirated leading to accumulation in scavenging macrophages led us to hypothesise that an activated population of LLM could interact with other immune cells to induce bronchial inf...
6 CitationsSource
#1Kiichi Nakahira (Cornell University)H-Index: 39
#2Maria Angelica Pabon Porras (Cornell University)H-Index: 2
Last. Augustine M.K. Choi (Cornell University)H-Index: 129
view all 3 authors...
(Macro)autophagy provides a membrane-dependent mechanism for the sequestration, transport, and lysosomal turnover of subcellular components, including proteins and organelles. In this capacity, autophagy maintains basal cellular homeostasis and healthy organelle populations such as mitochondria. During starvation, autophagy prolongs cell survival by recycling metabolic precursors from intracellular macromolecules. Furthermore, autophagy represents an inducible response to chemical and physical c...
38 CitationsSource
#1Thomas Lahiri (Boston Children's Hospital)H-Index: 1
4 CitationsSource
#1Alyn H. Morice (University of Hull)H-Index: 35
#2Shoaib Faruqi (University of Hull)H-Index: 15
Last. J. M. Bland (Ebor: University of York)H-Index: 1
view all 5 authors...
We postulate that most patients with chronic cough have a single discrete clinical entity: Cough Hypersensitivity Syndrome. We constructed a questionnaire that elicits the major components of the syndrome. Here we describe the validation of this questionnaire. Following iterative development, the Hull Airway Reflux Questionnaire (HARQ) was administered to patients and normal volunteers. It is self-administered and comprises 14 items with a maximum score of 70. Unselected patients were recruited ...
124 CitationsSource
#1John L. Colombo (UNMC: University of Nebraska Medical Center)H-Index: 21
#2Timothy K. Hallberg (UNMC: University of Nebraska Medical Center)H-Index: 5
We prospectively evaluated bronchial washings from 45 patients aged 1 month to 25 years (mean 3.3 years) for the presence of lipid-laden macrophages. By grading the amount of intracellular oil-red-O-positive material, we determined a semiquantitative lipid-laden macrophage index for each patient. All patients observed to be definite aspirators (n = 22) had indexes ≥86 (mean ± SD, 139 ± 46). All patients with no clinical suspicion of aspiration (n = 23) had indexes ⩽72 (mean ± SD, 21 ± 20). Our r...
140 CitationsSource
Cited By1
#1Laura R. Sadofsky (Hull York Medical School)H-Index: 12
#2Yvette A. Hayman (Hull York Medical School)H-Index: 3
Last. Alyn H. Morice (Hull York Medical School)H-Index: 2
view all 9 authors...
Purpose There is currently no true macrophage cell line and in vitro experiments requiring these cells currently require mitogenic stimulation of a macrophage precursor cell line (THP-1) or ex vivo maturation of circulating primary monocytes. In this study, we characterise a human macrophage cell line, derived from THP-1 cells, and compare its phenotype to the THP-1 cells.
1 CitationsSource